Detection of Apple stem grooving virus in different tissues of apple trees throughout the year

https://doi.org/10.17221/3863-PPSCitation:Kundu J.K., Svoboda J., Polák J. (2003): Detection of Apple stem grooving virus in different tissues of apple trees throughout the year. Plant Protect. Sci., 39: 93-96.
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The suitability of different apple tissues for Apple stem grooving virus (ASGV) detection throughout the year was checked by RT-PCR and ELISA. Detectable amounts of ASGV were generally found in all tissues (bark, dormant buds, petals and leaves) tested by RT-PCR from January to mid-June. Leaves during flowering (in May) were the most suitable tissues for the virus detection by both methods (RT-PCR and ELISA). The leaves collected in summer (June, July and August) or other tissues such as bark, dormant buds and petals were not reliable for ASGV detection by ELISA.  

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