Identification of Xiphinema vuittenezi by polymerase chain reaction
Shesh Kumari, Jiban K. Kundu, Jaroslav Polákhttps://doi.org/10.17221/3120-PPSCitation:Kumari S., Kundu J.K., Polák J. (2004): Identification of Xiphinema vuittenezi by polymerase chain reaction. Plant Protect. Sci., 40: 1-4.
So far, the identification of the nematode species Xiphinema vuittenezi relied mainly on time-consuming morphological and morphometrical studies. Therefore, a polymerase chain reaction (PCR) protocol was optimised that both reliably and rapidly identifies X. vuittenezi. The internal transcribed spacer (ITS) species-specific primer of ribosomal DNA gene of X. vuittenezi was used. Nine populations of this species from Central Bohemia were investigated by means of PCR.
nematodes; Xiphinema vuittenezi; PCR; ITS primer