Natural prevalence of entomopathogenic fungi in hibernating pupae of Cameraria ohridella (Lepidoptera: Gracillariidae) and virulence of selected isolates R., Chládeková P., Medo J., Barta M. (2016): Natural prevalence of entomopathogenic fungi in hibernating pupae of Cameraria ohridella (Lepidoptera: Gracillariidae) and virulence of selected isolates. Plant Protect. Sci., 52: 199-208.
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Spontaneous infection of hibernating Cameraria ohridella pupae by entomopathogenic fungi was monitored at two localities of south-western Slovakia and efficacy of selected fungal isolates against the pupae was evaluated in laboratory. Natural prevalence of fungal infection in pupal populations was low (< 7%) and varied depending upon collecting date, locality, year, and pathogen. Ten isolates of three fungal species, Beauveria pseudobassiana, Isaria fumosorosea, and I. farinosa, isolated from the naturally infected pupae and three isolates of non-insect origin of two fungi, B. bassiana and Metarhizium anisopliae, were screened for colony growth, conidial production in vitro, and virulence to C. ohridella pupae. A significant variability in the evaluated traits was detected among the isolates. Pupae demonstrated vulnerability to all the isolates irrespective of their origin, however virulence of isolates varied significantly. I. fumosorosea was the most virulent fungus with median lethal concentration of 0.09 × 104 conidia/ml (isolate CO10-IFu) and mean survival time of pupae of 7.14 days (isolate CO8-IFu). No correlation between conidial yield of isolates and fungal virulence was observed, but a moderately strong relationship was detected between virulence and mycelial growth rate of isolates.
Altschul S. (): Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389-3402
Augustin S. (2009): Cameraria ohridella Deschka & Dimić, horse chestnut leaf-miner (Gracillariidae, Lepidoptera). Species accounts of 100 of the most invasive alien species in Europe. In: Drake J.A. (ed.): Handbook of Alien Species in Europe. Invading Nature Springer Series in Invasion Ecology, Vol. 3. Knoxville, Springer: 331.
Augustin S., Guichard S., Heitland W., Freise J., Svatoš A., Gilbert M. (2009): Monitoring and dispersal of the invading Gracillariidae Cameraria ohridella. Journal of Applied Entomology, 133, 58-66
Backhaus G.F., Wulf A., Kehr R., Schröder T. (2002): Die Rosskastanien-Miniermotte (Cameraria ohridella) – Biologie Verbreitung und Gegenmassnahmen. Nachrichtenblatt für den Deutschen Pflanzenschutzdienst, 54: 56–62.
Barta M. (2010): Pathogenicity assessment of entomopathogenic fungi infecting Leptoglossus occidentalis (Heteroptera: Coreidae). Czech Mycology, 62: 67–78.
Barta M. (2011): Využitie metód digitálnej obrazovej analýzy na vyhodnotenie poškodenia listovej plochy duba japonského, Quercus aliena Blume, fyloxérou, Phylloxera quercina (Ferrari, 1872). In: Dreviny vo verejnej zeleni, May 17–18, 2011, Nitra, Slovakia: 16–22.
Bidochka Michael J, Kasperski June E, Wild Geoffrey AM (1998): Occurrence of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana in soils from temperate and near-northern habitats. Canadian Journal of Botany, 76, 1198-1204
D'Costa Lilla, Simmonds Monique S.J., Straw Nigel, Castagneyrol Bastien, Koricheva Julia (2014): Leaf traits influencing oviposition preference and larval performance of Cameraria ohridella on native and novel host plants. Entomologia Experimentalis et Applicata, 152, 157-164
Deschka G., Dimić N. (1986): Cameraria ohridella sp. n. aus Mazedonien, Jugoslawien (Lepidoptera; Lithocolletidae). Acta Entomologica Jugoslaviae, 22 (1–2): 11–23.
Finney D.J. (1971): Probit Analysis. 3rd Ed. London, Cambridge University Press.
Gilbert M., Svatos A., Lehmann M., Bacher S. (2003): Spatial patterns and infestation processes in the horse chestnut leafminer Cameraria ohridella: a tale of two cities. Entomologia Experimentalis et Applicata, 107, 25-37
GILBERT M., GUICHARD S., FREISE J., GRÉGOIRE J.-C., HEITLAND W., STRAW N., TILBURY C., AUGUSTIN S. (2005): Forecasting Cameraria ohridella invasion dynamics in recently invaded countries: from validation to prediction. Journal of Applied Ecology, 42, 805-813
Goettel M.S., Poprawski T.J., Vandenberg J.D., Li Z., Roberts D.W. (1990): Safety to nontarget invertebrates of fungal biocontrol agents. In: Laird M., Lacey L.A., Davidson E.W. (eds): Safety of Microbial Insecticides. Boca Raton, CRC Press: 209–231.
Grabenweger G. (2003): Parasitism of different larval stages of Cameraria ohridella. Biocontrol, 48: 671–684.
Grabenweger G., Avtzis N., Girardoz S., Hrasovec B., Tomov R., Kenis M. (2005a): Parasitism of Cameraria ohridella (Lepidoptera, Gracillariidae) in natural and artificial horse-chestnut stands in the Balkans. Agricultural and Forest Entomology, 7: 291–296.
GRABENWEGER Giselher, HOPP Hildegard, JACKEL Barbara, BALDER Hartmut, KOCH Tanja, SCHMOLLING Silke (2007): Impact of poor host-parasitoid synchronisation on the parasitism of Cameraria ohridella (Lepidoptera: Gracillariidae). European Journal of Entomology, 104, 153-158
Grabenweger G., Kehrli P., Schlick-Steiner B., Steiner F., Stolz M., Bacher S. (2005b): Predator complex of the horse chestnut leafminer Cameraria ohridella: identification and impact assessment. Journal of Applied Entomology, 129: 353–362.
Humber R.A. (2012): Identification of entomopathogenic fungi. In: Lacey L.A. (ed.): Manual of Techniques in Invertebrate Pathology,. 2nd Ed. Amsterdam, Academic Press: 151–187.
Jones W. E., Grace J. K., Tamashiro M. (1996): Virulence of Seven Isolates of Beauveria bassiana and Metarhizium anisopliae to Coptotermes formosanus (Isoptera: Rhinotermitidae). Environmental Entomology, 25, 481-487
Kalmus M., Sermann H., Jäckel B., Büttner C. (2008): Effect of the entomopathogenic fungi Lecanicillium muscarium and Paecilomyces fumosoroseus on the mortality of eggs and larvae of Cameraria ohridella Deschka & Dimic, 1986. In: Inglese P., Bedlan G. (eds): Proceedings of papers. 1st Symposium on Horticulture in Europe, Febr 17–20, 2008, University of Vienna – Biozentrum, Austria: 51–52.
Kaplan E. L., Meier Paul (1958): Nonparametric Estimation from Incomplete Observations. Journal of the American Statistical Association, 53, 457-481
Kehrli P., Bacher S. (2003): Date of leaf litter removal to prevent emergence of Cameraria ohridella in the following spring. Entomologia Experimentalis et Applicata, 107, 159-162
Kenis M., Tomov R., Svatos A., Schlinsog P., Lopez Vaamonde C., Heitland W., Grabenweger G., Girardoz S., Freise J., Avtzis N. (2005): The horse-chestnut leaf miner in Europe – Prospects and constraints for biological control. In: Hoddle M.S. (ed.): Proceedings of papers. 2nd International Symposium on Biological Control of Arthropods, Sept 12–16, 2005, USDA Forest Service, Davos, USA: 77–90.
Kindl J., Kalinová B., Freise J., Heitland W., Augustin S., Guichard S., Avtzis N., Svatoš A. (2002): Monitoring the population dynamics of the horse chestnut leafminer Cameraria ohridella with a synthetic pheromone in Europe. Plant Protection Science, 38: 131–138.
Kuldová J., Hrdý I., Janšta P. (2007): The horse chestnut leafminer Cameraria ohridella: chemical control and notes on parasitisation. Plant Protection Science, 43: 47–56.
Medo J. (2009): Druhové spektrum entomopatogénnych húb z radu Hypocreales izolovaných z pôdnych vzoriek pochádzajúcich z rôznych lokalít Slovenska a genetická štruktúra populácií Beauveria bassiana. [PhD Dissertation.] Nitra, Slovak University of Agriculture in Nitra.
Medo Juraj, Cagáň Ľudovít (2011): Factors affecting the occurrence of entomopathogenic fungi in soils of Slovakia as revealed using two methods. Biological Control, 59, 200-208
Metla Z., Voitkāne S., Sešķēna R., Petrova V., Jankevica L. (2013): Presence of entomopathogenic fungi and bacteria in Latvian population of horse-chestnut leaf miner Cameraria ohridella. Acta Biologica Universitatis Daugavpiliensis, 13: 69–76.
Pavan F., Barro P., Bernardinelli I., Gambon N., Zandigiacomo P. (2003): Cultural control of Cameraria ohridella on horse chestnut in urban areas by removing fallen leaves in autumn. Journal of Arboriculture, 29: 253–258.
Pérez-González Víctor H., Guzmán-Franco Ariel W., Alatorre-Rosas Raquel, Hernández-López Jorge, Hernández-López Antonio, Carrillo-Benítez María G., Baverstock Jason (2014): Specific diversity of the entomopathogenic fungi Beauveria and Metarhizium in Mexican agricultural soils. Journal of Invertebrate Pathology, 119, 54-61
Prenerová E., Zemek R., Weyda F. (2008): The first record of Beauveria bassiana (Deuteromycetes) on the hibernating pupae of Cameraria ohridella (Lepidoptera: Gracillariidae). In: 41st Annual Meeting of SIP and 9th International Conference on Bacillus thuringiensis, 3–7 August 2008, University of Warwick, UK. Program and Abstracts: 63.
Prenerová E., Zemek R., Weyda F. (2009a): Pathogenicity of blastospores and conidia of Isaria fumosorosea against pupae of the horse chestnut leaf-miner, Cameraria ohridella. Online Journal of Scientific Posters. Available at (accessed July 5, 2015).
Prenerová E., Zemek R., Weyda F., Volter L., původce; Biologické Centrum AV ČR, v. v. i., majitel patentu. 22. červenec 2009b. Kmen entomopatogenní houby Isaria fumosorosea CCM 8367 (CCEF0.011.PFR) a způsob likvidace hmyzích škůdců a roztočů. CR patent 300 701.
Prenerová E., Zemek R., Volter L., Weyda F., inventors; Biology Centre AS CR, v.v.i., asignee. 2013 Nov 5. Strain of entomopathogenic fungus Isaria fumosorosea CCM 8367 (CCEF0.011.PFR) and the method for controlling insect and mite pests. U.S. patent 8,574,566 B2.
Quesada-Moraga Enrique, Navas-Cortés Juan A., Maranhao Elizabeth A.A., Ortiz-Urquiza Almudena, Santiago-Álvarez Cándido (2007): Factors affecting the occurrence and distribution of entomopathogenic fungi in natural and cultivated soils. Mycological Research, 111, 947-966
Rämert B., Kenis M., Kärnestam E., Nyström M., Rännbäck L.M. (2011): Host plant suitability, population dynamics and parasitoids of the horse chestnut leafminer Cameraria ohridella (Lepidoptera: Gracillariidae) in southern Sweden. Acta Agriculturae Scandinavica, Section B – Soil and Plant Science, 61: 480–486.
Rehner S. A., Minnis A. M., Sung G.-H., Luangsa-ard J. J., Devotto L., Humber R. A. (): Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria. Mycologia, 103, 1055-1073
Richter D., Sermann H., Jäckel B., Büttner C. (2007a): Pathogenicity of entomopathogenic fungi on hibernating pupae of Cameraria ohridella Deschka & Dimic 1986 (Lepidoptera, Gracillariidae). Part 1: Pathogenicity against the naked pupa. Communications in Agricultural and Applied Biological Sciences, 72: 399–410.
Richter D., Sermann H., Jäckel B., Büttner C. (2007b): Pathogenicity of entomopathogenic fungi on hibernating pupae of Cameraria ohridella Deschka & Dimic 1986 (Lepidoptera, Gracillariidae). Part 2: Efficacy of entomopathogenic fungi against pupa in her pupal cell. Communications in Agricultural and Applied Biological Sciences, 72: 411–421.
Richter D., Sermann H., Jäckel B., Büttner C. (2007c): Efficiency of entomopathogenic fungi for pupae of the horse chestnut leafminer Cameraria ohridella in the winter. In: Proceedings of abstracts. 11th European Meeting of the IOBC/wprs Working Group Insect Pathogens and Insect Parasitic Nematodes, Aug 29, 2007, Alès, Schwitzerland: 105.
Richter D., Sermann H., Jäckel B., Büttner C. (2008): Efficiency of the entomopathogenic fungus Lecanicillium muscarium on hibernating pupae of Cameraria ohridella Deschka & Dimic, 1986 (Lepid., Gracillariidae). IOBC/WPRS Bulletin, 31: 223–227.
Salleo S., Nardini A., Raimondo F., Lo Gullo M.A., Pace F., Giacomich P. (2003): Effects of defoliation caused by the leaf miner Cameraria ohridella on wood reduction and efficiency in Aesculus hippocastanum growing in north-eastern Italy. Trees, 17: 367–375.
Sambrook J., Fritsch E.F., Maniatis T. (1989): Molecular Cloning: A Laboratory Manual. Vol. 2. 2nd Ed. New York, Cold Spring Harbor Laboratory Press.
Samek T., Novotný D., Jankovský L. (2006): Infection of wintering pupae of horse-chestnut leafminer Cameraria ohridella Deschka et Dimić. by Verticillium lecanii (Zimmerman) Viégas. Journal of Forest Science, 52: 136–140.
Shapiro-Ilan David I., Cottrell Ted E., Jackson Mark A., Wood Bruce W. (2008): Virulence of Hypocreales fungi to pecan aphids (Hemiptera: Aphididae) in the laboratory. Journal of Invertebrate Pathology, 99, 312-317
Sierpinska A., Kubiak K. (2011): Entomopathogenic fungal infections of hibernating pupae of horse chestnut moth Cameraria ohridella Deschka & Dimic. IOBC/WPRS Bulletin, 66: 186.
Šefrová H., Laštůvka Z. (2001): Dispersal of the horse-chestnut leafminer, Cameraria ohridella Deschka & Dimić, 1986, in Europe: its course, ways and causes (Lepidoptera: Gracillariidae). Entomologische Zeitschrift, Stuttgart, 111: 194–198.
Talaei -Hassanloui Reza, Kharazi-Pakdel Aziz, Goettel Mark, Mozaffari Javad (2006): Variation in virulence of Beauveria bassiana isolates and its relatedness to some morphological characteristics. Biocontrol Science and Technology, 16, 525-534
Valero-Jiménez Claudio A., Wiegers Harm, Zwaan Bas J., Koenraadt Constantianus J.M., van Kan Jan A.L. (2016): Genes involved in virulence of the entomopathogenic fungus Beauveria bassiana. Journal of Invertebrate Pathology, 133, 41-49
Vänninen Irene (1996): Distribution and occurrence of four entomopathogenic fungi in Finland: effect of geographical location, habitat type and soil type. Mycological Research, 100, 93-101
Varela Amanda, Morales Esperanza (1996): Characterization of SomeBeauveria bassianaIsolates and Their Virulence toward the Coffee Berry BorerHypothenemus hampei. Journal of Invertebrate Pathology, 67, 147-152
Vega F.E., Meyling N.V., Luangsa-ard J.J., Blackwell M. (2012): Chapter 6 – Fungal Entomopathogens. In: Vega F.E., Kaya H.K. (eds): Insect Pathology. 2nd Ed. San Diego, Academic Press: 171–220.
Zemek R., Prenerová E., Volter L., Weyda F., Skuhravý V. (2007): Perspectives for the biological control of Cameraria ohridella. Communications in Agricultural and Applied Biological Sciences, 72: 521–527.
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