Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeri A., Tenuta M., Gulden R.H. (2017): Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeri. Plant Protect. Sci., 53: 254-264.
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Ditylenchus weischeri was recently reported in the provinces of Manitoba and Saskatchewan, Canada. Populations of D. weischeri from creeping thistle (Cirsium arvense L.) in Manitoba and D. dipsaci from garlic (Allium cepa L.) in Ontario were examined for their potential to grow on callused carrot (Daucus carota subsp. sativus) disks, alfalfa (Medi-
cago sativa
L.) and creeping thistle callus tissues, and pure cultures of eight fungal species, Botrytis cinerea, Fusarium solani, Rhizoctonia solani, Verticillium dahliae, Sclerotinia sclerotiorum, Cladosporium cucumerinum, Colletotrichum gloeosporioides, and Chaetomium spp. Ditylenchus weischeri and D. dipsaci could not be reared on any of the fungal isolates nor in the callus tissues of creeping thistle. In contrast to D. weischeri, D. dipsaci was successfully reared on the alfalfa callus tissue. On the callused carrot disks, with no media, an increase of 54 and 244 times the initial density of 80 nematodes was obtained for D. weischeri and D. dipsaci, respectively. Monoxenic rearing was performed using callused carrot disks to provide sufficient D. weischeri inoculum for the microplot study. The effect of D. weischeri on yellow pea varieties Agassiz and Bronco was determined in a microplot trial using initial densities of 0, 100, 200, 400, 800, 1600, and 3200 nematodes/plant. While it had no impact on pea grain yield, D. weischeri slightly reduced plant height, aboveground biomass, and pod length at the population densities of 1600 and 3200 nematodes/plant. The final population densities at harvest were not significantly different from the initial densities indicating the pea varieties were poor hosts to D. weischeri. The results of the present study indicate that D. weischeri is unlikely to be a pest of yellow pea for weather conditions of the Canadian Prairies.

CABI (2015): Ditylenchus dipsaci. Invasive Species Compendium Datasheets, maps, images, abstracts and full text on invasive species of the world. Available at (accessed Apr 23, 2016).
Chizhov V.N., Borisov B.A., Subbotin S.A. (2010): A new stem nematode, Ditylenchus weischeri sp.n. (Nematoda: Tylenchida), a parasite of Cirsium arvense (L.) Scop. in the central region of the non-chernozem zone of Russia. Russian Journal of Nematology, 18: 95–102.
Eisenback J.D. (2000): Techniques for measuring nematode development and egg production. In: Wheeler T., Forge T., Caswell-Chen E. (eds): Techniques in Nematode Ecology. Hyatsville, Society of Nematologists: 16–19.
Faulkner L.R., Darling H.M. (1961): Pathological histology, hosts, and culture of the potato rot nematode. Phytopathology, 51: 778–786.
Griffin G.D. (1975): Parasitism of nonhost cultivars by Ditylenchus dipsaci. Journal of Nematology, 7: 236–238.
Hajihassani Abolfazl, Smiley Richard W., Afshar Farahnaz Jahanshahi (2013): Effects of Co-inoculation with Pratylenchus thornei and Fusarium culmorum on Growth and Yield of Winter Wheat. Plant Disease, 97, 1470-1477
Hajihassani Abolfazl, Tenuta Mario, Gulden Robert H. (2016): Host Preference and Seedborne Transmission of Ditylenchus weischeri and D. dipsaci on Select Pulse and Non-Pulse Crops Grown in the Canadian Prairies. Plant Disease, 100, 1087-1092
Hajihassani Abolfazl, Tenuta Mario, Gulden Robert H. (2017): Influence of Temperature on Development and Reproduction of Ditylenchus weischeri and D. dipsaci on Yellow Pea. Plant Disease, 101, 297-305
Hooper D.J., Southey J.F. (1978): Ditylenchus, Anguina and related genera. In: Southey J.F. (ed.): Plant Nematology. 3rd Ed. London, Ministry of Agriculture, Fisheries and Food: 78–79.
Hussey R.S., Krusberg L.R. (1971): Disc-electrophoretic patterns of enzymes and soluble proteins of Ditylenchus dipsaci and D. triformis. Journal of Nematology, 3: 79–84.
Kaplan D.T., Davis E.L. (1990): Improved nematode extraction from carrot disk culture. Journal of Nematology, 22: 399–406.
Krusberg L.R. (1961): Studies On the Culturing and Parasitism of Plant-Parasitic Nematodes, in Particular Ditylenchus Dipsaci and Aphelenchoides Ritzeiwabosi On Alfalfa Tissues. Nematologica, 6, 181-200
Blickenstaff Maxine L., Krusberg L.R. (1964): Influence of Plant Growth Regulating Substances On Reproduction of Ditylenchus Dipsaci, Pratylenchus Penetrans and Pratylenchus Zeae On Alfalfa Tissue Cultures 1). Nematologica, 10, 145-150
Kühnhold Volker, Kiewnick Sebastian, Sikora Richard A. (2006): Development of an <I>in vivo</I> bioassay to identify sugar beet resistance to the stem nematode <I>Ditylenchus dipsaci</I>. Nematology, 8, 641-645
LATIF M. A., MIAN I. H. (1995): Fungal Hosts and Gnotobiotic Culture of Ditylenchus angustus. Nematological Research (Japanese Journal of Nematology), 25, 11-15
Madani Mehrdad, Tenuta Mario, Chizhov Vladimir N., Subbotin Sergei A. (2015): Diagnostics of stem and bulb nematodes, Ditylenchus weischeri and D. dipsaci (Nematoda: Anguinidae), using PCR with species-specific primers. Canadian Journal of Plant Pathology, 37, 212-220
Manitoba Agriculture, Food and Rural Development (2010): The description of soil series in Manitoba. Available at (accessed Dec 10, 2015).
Moody E.H., Lownsbery B.E., Ahmed J.M. (1973): Culture of the root-lesion nematode Pratylenchus vulnus on carrot discs. Journal of Nematology, 5: 225–226.
Plowright R., Caubel G., Mizen K.A. (2002): Ditylenchus species. In: Starr J.L., Cook R., Bridge J. (eds): Plant Resistance to Parasitic Nematodes. Wallingford, CAB International: 107–139
Riedel R.M., Foster J.G. (1970): Monoxenic culture of Ditylenchus dipsaci and Pratylenchus penetrans with modified Krusberg’s and White’s media. Plant Disease Reporter, 54: 251–254.
Riedel R.M., Foster J.G., Mai W.F. (1973): A simplified medium for monoxenic culture of Pratylenchus penetrans and Ditylenchus dipsaci. Journal of Nematology, 5: 71.
Saskatchewan Pulse Growers. (2015): Growing peas. Available at (accessed Oct 4, 2016).
Saxton A.M. (1998): A macro for converting mean separation output to letter groupings in Proc Mixed. In: Book of Abstracts. 23rd SAS User Group International Conference, Cary. New York, SAS Institute: 1243–1246.
Sturhan D., Brzeski M.W. (1991): Stem and bulb nematodes, Ditylenchus spp. In: Nickle W.R. (ed.): Manual of Agricultural Nematology. New York, Marcel Dekker: 423–464.
Subbotin Sergei A., Madani Mehrdad, Krall Eino, Sturhan Dieter, Moens Maurice (2005): Molecular Diagnostics, Taxonomy, and Phylogeny of the Stem Nematode Ditylenchus dipsaci Species Complex Based on the Sequences of the Internal Transcribed Spacer-rDNA. Phytopathology, 95, 1308-1315
Mamiya Yasuharu, Tamura Hirotada (1975): Reproduction of Bursaphelenchus Lignicolus On Alfalfa Callus Tissues. Nematologica, 21, 449-454
Tenente R.V., Mendes M.A.S., Neto E.G. (1995): No reproduction of two populations of Ditylenchus dipsaci on three species of fungi. Nematologia Brasileira, 19: 67–71.
Tenuta M., Madani M., Briar S.H., Molina O., Gulden R.H., Subbotin S.A. (2014): Occurrence of Ditylenchus weischeri and not D. dipsaci in field pea harvest samples and Cirsium arvense in the Canadian Prairies. Journal of Nematology, 46: 376–384.
Verdejo-Lucas S., Pinochet J. (1992): Population densities of five migratory endoparasitic nematodes in carrot disk cultures. Journal of Nematology, 24: 96–98.
Viglierchio D.R. (1971): Race Genesis in Ditylenchus Dipsaci 1). Nematologica, 17, 386-392
Webster J. M., Lowe D. (1966): The effect of the synthetic plant-growth substance, 2, 4-dichlorophenoxyacetic acid, on the host–parasite relationships of some plant-parasitic nematodes in monoxenic callus culture. Parasitology, 56, 313-
WHITEHEAD A. G., HEMMING J. R. (1965): A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology, 55, 25-38
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