Detecting herbicide-resistant Apera spica-venti with a chlorophyll fluorescence agar test

https://doi.org/10.17221/110/2018-PSECitation:Linn A., Košnarová P., Soukup J., Gerhards R. (2018): Detecting herbicide-resistant Apera spica-venti with a chlorophyll fluorescence agar test. Plant Soil Environ., 64: 386-392.
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Reliable tests on herbicide resistance are important for resistance management. Despite well-established greenhouse bioassays, faster and in-season screening methods would aid in more efficient resistance detection. The feasibility of a chlorophyll fluorescence agar-based test on herbicide resistance in Apera spica-venti L. was investigated. Herbicide resistant and sensitive A. spica-venti seedlings were transplanted into agar containing pinoxaden and pyroxsulam herbicides. Chlorophyll fluorescence was measured and the maximum quantum efficiency of photosystem II (Fv/Fm) was determined 48 h and 72 h after the transplantation to agar, respectively. The Fv/Fm values decreased with increasing herbicide concentration. Dose-response curves and respective ED50 values (herbicide concentration leading to 50% decrease of the Fv/Fm value) were calculated. However, each experiment repetition exhibited different sensitivities of the populations for both herbicides. In certain cases, resistant populations demonstrated similar Fv/Fm values as sensitive populations. Contrary to the findings in Alopecurus myosuroides Huds., discrimination of sensitive and resistant A. spica-venti populations was not feasible. An increased importance of the assessment time due to the herbicide concentrations calibrated for fast responses was assumed in this study.

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