Soil enzyme activities after application of fungicide QuadrisR at increasing concentration rates

Boteva S.B., Kenarova A.E., Petkova M.R., Georgieva S.St., Chanev Ch.D., Radeva G.S. (2022): Soil enzyme activities after application of fungicide QuadrisR at increasing concentration rates. Plant Soil Environ., 68: 382–392.

download PDF

The study aimed to assess the effects of fungicide QuadrisR on activities of soil enzymes contributed to soil nutrient turnover. A batch laboratory experiment with QuadrisR-amended (0 mg/kg ds (dry soil) – 35.00 mg/kg ds) loamy sand soil was conducted, and shifts in soil physical environments and enzyme activities (beta-glucosidase, urease, acid and alkaline phosphatases, arylsulfatase and dehydrogenase) were evaluated on experimental days 1, 30, 60, 90 and 120. The results indicated that QuadrisR changed both soil properties and enzyme activities. The most sensitive environmental parameter to fungicide input was soil pH. The most suscaptable to QuadrisR enzymes were dehydrogenase and arylsulfatase, and the most resistant – urease. The mean overall dehydrogenase activity decreased by 33%, whereas the profile of arylsulfatase activity tended to permanent decrease over time. The general pattern of enzyme responses to QuadrisR was an immediate-early (days 1 – 30) decline of enzyme activities after fungicide application, except that of arylsulfatase. Beta-glucosidase manifested a temporal profile of steady-state stimulation under the lowest (2.90 mg/kg ds) and low sensitivity to the higher (14.65 mg/kg ds and 35.00 mg/kg ds) fungicide concentrations.

Aleksova M., Kenarova A., Boteva S., Georgieva S., Chanev Ch., Radeva G. (2021): Effects of increasing concentrations of fungicide QuadrisR on bacterial functional profiling in loamy sand soil. Archives of Microbiology, 203: 4385–4396.
Álvarez-Martín A., Hilton S.L., Bending G.D., Rodríguez-Cruz M.S., Sánchez-Martín M.J. (2016): Changes in activity and structure of the soil microbial community after application of azoxystrobin or pirimicarb and an organic amendment to an agricultural soil. Applied Soil Ecology, 106: 47–57.
Anonymous (2016): China Strobilurin Fungicides Market Report 2016 Edition – Research and Markets. Available at: (accessed 31. 1. 2022)
Baćmaga M., Kucharski J., Wyszkowska J. (2015): Microbial and enzymatic activity of soil contaminated with azoxystrobin. Environmental Monitoring and Assessment, 187: 615.
Baćmaga M., Wyszkowska J., Kucharski J. (2020): Response of soil microorganisms and enzymes to the foliar application of Helicur 250 EW fungicide on Horderum vulgare L. Chemosphere, 242: 125163.
Bartlett D.W., Clough J.M., Godwin J.R., Hall A.A., Hamer M., Parr-Dobrzanski B. (2002): The strobilurin fungicides. Pest Management Science, 58: 649–662.
Bending G.D., Rodríguez-Cruz M.S., Lincoln S.D. (2007): Fungicide impacts on microbial communities in soils with contrasting management histories. Chemosphere, 69: 82–88.
Burrows L.A., Edwards C.A. (2004): The use of integrated soil microcosms to assess the impact of carbendazim on soil ecosystems. Ecotoxicology, 13: 143–161.
Chen S.K., Edwards C.A., Subler S. (2001): Effects of the fungicides benomyl, captan and chlorothalonil on soil microbial activity and nitrogen dynamics in laboratory incubations. Soil Biology and Biochemistry, 33: 1971–1980.
Cycoń M., Piotrowska-Seget Z., Kozdrój J. (2010): Responses of indigenous microorganisms to a fungicidal mixture of mancozeb and dimethomorph added to sandy soils. International Biodeterioration and Biodegradation, 64: 316–323.
Eivazi F., Tabatabai M.A. (1988): Glucosidases and galactosidases in soils. Soil Biology and Biochemistry, 20: 601–606.
Floch C., Chevremont A., Joanico K., Capowiez Y., Criquet S. (2011): Indicators of pesticide contamination: soil enzyme compared to functional diversity of bacterial communities via Biolog® Ecoplates. European Journal of Soil Biology, 47: 256–263.
Friedel J.K., Mölter K., Fischer W.R. (1994): Comparison and improvement of methods for determining soil dehydrogenase activity by using triphenyltetrazolium chloride and iodonitrotetrazolium chloride. Biology and Fertility of Soils, 18: 291–296.
Ghosh R.K., Singh N. (2009): Effect of organic manure on sorption and degradation of azoxystrobin in soil. Journal of Agricultural and Food Chemistry, 57: 632–636.
Guo P., Zhu L., Wang J., Wang J., Xie H., Lv D. (2015): Enzymatic activities and microbial biomass in black soil as affected by azoxystrobin. Environmental Earth Sciences, 72: 1353–1361.
Hammer Ø., Harper D.A.T., Ryan P.D. (2001): PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4: 1–9.
Howell C.C., Semple K.T., Bending G.D. (2014): Isolation and characterization of azoxystrobin degrading bacteria from soil. Chemosphere, 95: 370–378.
Kandeler E., Gerber H. (1988): Short-term assay of soil urease activity using colorimetric determination of ammonium. Biology and Fertility of Soils, 6: 68–72.
Keeney D.R., Nelson D.W. (1982): Nitrogen-inorganic forms. In: Page A.L., Miller R.H., Keeney D. (eds.): Methods of Soil Analysis, Part 2, Agronomy Monograph 9. Madison, American Society of Agronomy and Soil Science Society of America, 643–698.
Köhler H.R., Triebskorn R. (2013): Wildlife ecotoxicology of pesticides: can we track effects to the population level and beyond? Science, 341: 759–765.
Lavelle P., Spain A.V. (2005): Chapter 3: Soil Organisms. In: Lavelle P., Spain A.V. (eds.): Soil Ecology. Dordrecht, Springer, 201–356. ISBN: 9780306481628
Mijangos I., Becerril J.M., Albizu I., Epelde L., Garbisu C. (2009): Effects of glyphosate on rhizosphere soil microbial communities under two different plant composition by cultivation-dependent and -independent methodologies. Soil Biology and Biochemistry, 41: 505–513.
Monkiedje A., Ilori M.O., Spiteller M. (2002): Soil quality changes resulting from the application of the fungicides mefenoxam and metalaxyl to a sandy loam soil. Soil Biology and Biochemistry, 34: 1939–1948.
Muñoz-Leoz B., Ruiz-Romera E., Antigüedad I., Garbisu C. (2011): Tebuconazole application decreases soil microbial biomass and activity. Soil Biology and Biochemistry, 43: 2176–2183.
Muñoz-Leoz B., Garbisu C., Charcosse J.Y., Sánchez-Pérez J.M., Antigüedad I., Ruiz-Romera E. (2013): Non-target effects of three formulated pesticides on microbially-mediated processes in a clay-loam soil. Science of the Total Environment, 449: 345–354.
Olsen S.R. (1982): Phosphorus. In: Page A.L., Miller R.H., Keeney D. (eds.): Methods of Soil Analysis, Part 2, Agronomy Monograph 9. Madison, American Society of Agronomy and Soil Science Society of America, 1040–1042.
Puglisi E., Vasileiadis S., Demiris K., Bassi D., Karpouzas D.G., Capri E., Cocconcelli P.S., Trevisan M. (2012): Impact of fungicides on the diversity and function of non-target ammonia-oxidizing microorganisms residing in a litter soil cover. Microbial Ecology, 64: 692–701.
Riah W., Laval K., Laroche-Ajzenberg E., Mougin Ch., Latour X., Trinsoutrot-Gattin I. (2014): Effects of pesticides on soil enzymes: a review. Environmental Chemistry Letters, 12: 257–273.
Ruske R.E., Gooding M.J., Dobraszczyk B.J. (2004): Effects of Triazole and Strobilurin fungicide programmes, with and without late-sea son nitrogen fertilizer, on the baking quality of Malacca winter wheat. Journal of Cereal Science, 40: 1–8.
Siwik-Ziomek A., Lemanowicz J., Koper J. (2016): Arylsulphatase activity and sulphate content in relation to crop rotation and fertilization of soil. International Agrophysics, 30: 359–367.
Sopeña F., Bending G.D. (2013): Impacts of biochar on bioavailability of the fungicide azoxystrobin: a comparison of the effect on biodegradation rate and toxicity to the fungal community. Chemosphere, 91: 1525–1533.
Sukul P. (2006): Enzymatic activities and microbial biomass in soil as influenced by metalaxyl residues. Soil Biology and Biochemistry, 38: 320–326.
Tabatabai M.A., Bremner J.M. (1969): Use of p-nitrophenylphosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry, 1: 301–307.
Tabatabai M.A., Bremner J.M. (1970): Arylsulfatase activity of soils. Soil Science Society of America Journal, 34: 225–229.
Utobo E.B., Tewari L. (2015): Soil enzymes as bioindicators of soil ecosystem status. Applied Ecology and Environmental Research, 13: 147–169.
Waldrop M.P., Balser T.C., Firestone M.K. (2000): Linking microbial community composition to function in a tropical soil. Soil Biology and Biochemistry, 32: 1837–1846.
Wang Y.S., Wen C.Y., Chiu T.C., Yen J.H. (2004): Effect of fungicide iprodione on soil bacterial community. Ecotoxicology and Environmental Safety, 59: 127–132.
Wang C., Wang F., Zhang Q., Liang W. (2016): Individual and combined effects of tebuconazole and carbendazim on soil microbial activity. European Journal of Soil Biology, 72: 6–13.
Wang F., Li X., Zhu L., Du Z., Zhang C., Wang J., Wang J., Lv D. (2018): Responses of soil microorganisms and enzymatic activities to azoxystrobin in Cambisol. Polish Journal of Environmental Studies, 27: 2775–2783.
Wang X., Lu Z., Miller H., Liu J., Hou Z., Liang S., Zhao X., Zhang H., Borch T. (2020): Fungicide azoxystrobin induced changes on the soil microbiome. Applied Soil Ecology, 145: 103343.
Xiong D., Li Y., Xiong Y., Li X., Xiao Y., Qin Z., Xiao Y. (2014): Influence of boscalid on the activities of soil enzymes and soil respiration. European Journal of Soil Biology, 61: 1–5.
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti