Soil microorganisms play a main role in the nutrient cycle and they also play an important role in soil health. This article studies the influence of three rates of biochar (0.5, 1 and 3%) in comparison with control (0 biochar) in two different soils (Valečov and Čistá) on soil microbiota activities. The biochar was prepared from 80% of digestate from Zea mays L. and 20% of cellulose fibres by pyrolysis (470°C, 17 min). The biochar ability to influence microbial processes in soil was determined by respiration and nitrification tests. There were no significant differences between basal respiration of control samples and biochar-amended samples. Basal respiration in the Valečov soil reached average amounts from 1.32 to 1.52 mg CO2/h/100 g. In the Čistá soil, basal respiration reached average amounts from 1.40 to 1.49 mg CO2/h/100 g. No significant differences were proved also in nitrification tests of both soils. Nitrifying potential was the highest in 3% rate of biochar amendment. There were no negative changes in the measured soil parameters. CO2 efflux was not higher in biochar-amended soil.
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