Effects of nitrogen addition on root traits and soil nitrogen in the long-term restored grasslands


Jing G.H., Chen Z.K., Lu Q.Q., He L.Y., Zhao N., Zhang Z., Li W. (2021): Effects of nitrogen addition on root traits and soil nitrogen in the long-term restored grasslands. Plant Soil Environ., 67: 541–547.


download PDF

Fine root traits are plastic and responsive to increased nitrogen (N) deposition. However, with the restoring of the ecosystem after grain for green, little research has been reported about the response of root traits in a long-term restored ecosystem to increased N deposition. Therefore, a successive N addition experiment was conducted in a long-term restored grassland on the Loess Plateau to analyse the effects of different N addition levels (0, 2.5, 5, 10, 20 g N/m2/year) on root morphological traits, soil carbon (C) and N. Our results showed that root morphological traits (except for root diameter) firstly increased and then declined, with the maximum in the N level of 5 g/m2/year. N addition significantly increased soil organic carbon, total nitrogen, ammonium nitrogen (NH4+-N) and nitrate-nitrogen (NO3-N) with the increasing N addition level, especially in the soil surface layer. Specific root length and specific root area had remarkable negative correlations with NO3-N, while root diameter and root length density had positive correlations with soil availability N and soil microbial biomass carbon. This study indicated that plants could have the threshold response to adapt to the N addition and prefer to slowly grow rather than quickly invest and return in order to adapt to the environmental stress.


Bai Y.F., Wu J.G., Clark C.M., Naeem S., Pan Q.M., Huang J.H., Zhang L.X., Han X.G. (2010): Tradeoffs and thresholds in the effects of nitrogen addition on biodiversity and ecosystem functioning: evidence from inner Mongolia Grasslands. Global Change Biology, 16: 358–372. https://doi.org/10.1111/j.1365-2486.2009.01950.x
Bejarano M., Etchevers J.D., Ruíz-Suárez G., Campo J. (2014): The effects of increased N input on soil C and N dynamics in seasonally dry tropical forests: an experimental approach. Applied Soil Ecology, 73: 105–115. https://doi.org/10.1016/j.apsoil.2013.08.015
Chen H., Li D.J., Gurmesa G.A., Yu G.R., Li L.H., Zhang W., Fang H.J., Mo J.M. (2015a): Effects of nitrogen deposition on carbon cycle in terrestrial ecosystems of China: a meta-analysis. Environmental Pollution, 206: 352–360. https://doi.org/10.1016/j.envpol.2015.07.033
Chen D.M., Lan Z.C., Hu S.J., Bai Y.F. (2015b): Effects of nitrogen addition on belowground communities in grassland: relative role of soil nitrogen availability vs. soil acidification. Soil Biology and Biochemistry, 89: 99–108. https://doi.org/10.1016/j.soilbio.2015.06.028
Chen G.T., Tu L.H., Peng Y., Hu H.L., Hu T.X., Xu Z.F., Liu L., Tang Y. (2016): Effect of nitrogen additions on root morphology and chemistry in a subtropical bamboo forest. Plant and Soil, 412: 441–451. https://doi.org/10.1007/s11104-016-3074-z
Craig H., Antwis R.E., Cordero I., Ashworth D., Robinson C.H., Osborne T.Z., Bardgett R.D., Rowntree J.K., Simpson L.T. (2021): Nitrogen addition alters composition, diversity, and functioning of microbial communities in mangrove soils: an incubation experiment. Soil Biology and Biochemistry, 153: 108076. https://doi.org/10.1016/j.soilbio.2020.108076
Dijkstra F.A., Hobbie S.E., Reich P.B., Knops J.M.H. (2005): Divergent effects of elevated CO2, N fertilization, and plant diversity on soil C and N dynamics in a grassland field experiment. Plant and Soil, 272: 41–52. https://doi.org/10.1007/s11104-004-3848-6
Fornara D.A., Banin L., Crawley M.J. (2013): Multi-nutrient vs. nitrogen-only effects on carbon sequestration in grassland soils. Global Change Biology, 19: 3848–3857. https://doi.org/10.1111/gcb.12323
Galloway J.N., Dentener F.J., Capone D.G., Boyer E.W., Howarth R.W., Seitzinger S.P., Asner G.P., Cleveland C.C., Green P.A., Holland E.A., Karl D.M., Michaels A.F., Porter J.H., Townsend A.R., Vöosmarty C.J. (2004): Nitrogen cycles: past, present, and future. Biogeochemistry, 70: 153–226. https://doi.org/10.1007/s10533-004-0370-0
Gao W.L., Yang H., Kou L., Li S.G. (2015): Effects of nitrogen deposition and fertilization on N transformations in forest soils: a review. Journal of Soils Sediments, 15: 863–879. https://doi.org/10.1007/s11368-015-1064-z
IPCC Climate Change (2013): The physical science basis. Contribution of working group I to the fifth assessment report of the intergovernmental panel on climate change. In: Stocker T.F., Qin D., Plattner G.-K., Tignor M., Allen S.K., Boschung J., Nauels A., Xia Y., Bex V., Midgley P.M. (eds.): Summary for Policymakers. Intergovernmental Panel on Climate Change. Cambridge, New York, Cambridge University Press, 1535.
Leuschner C., Gebel S., Rose L. (2013): Root trait responses of six temperate grassland species to intensive mowing and NPK fertilisation: a field study in a temperate grassland. Plant and Soil, 373: 687–698. https://doi.org/10.1007/s11104-013-1836-4
Liu H.F., Xue S., Wang G.L., Xin Q., Liu G.B. (2016): Effects of N addition on different organic carbon fractions of soil growing Bothriochloa ischaemum. Acta Agrestia Sinica, 24: 939–946.
Liu W.X., Xu W.H., Han Y., Wang C.H., Wan S.Q. (2007): Responses of microbial biomass and respiration of soil to topography, burning, and nitrogen fertilization in a temperate steppe. Biology and Fertility of Soils, 44: 259–268. https://doi.org/10.1007/s00374-007-0198-6
López-Angulo J., de la Cruz M., Chacón-Labella J., Illuminati A., Matesanz S., Pescador D.S., Pías B., Sánchez A.M., Escudero A. (2020): The role of root community attributes in predicting soil fungal and bacterial community patterns. New Phytologist, 228: 1070–1082. https://doi.org/10.1111/nph.16754
Ma J.Y., Kang F.F., Cheng X.Q., Han H.R. (2018): Response of soil organic carbon and nitrogen to nitrogen deposition in a Larix principis-rupprechtii plantation. Scientific Reports, 8: 8638. https://doi.org/10.1038/s41598-018-26966-5
Meinen C., Hertel D., Leuschner C. (2009): Biomass and morphology of fine roots in temperate broad-leaved forests differing in tree species diversity: is there evidence of below-ground over- https://doi.org/10.1007/s00442-009-1352-7
yielding? Oecologia, 161: 99–111.
Qin J., Bao Y.J., Li Z.H., Hu Z.C., Gao W. (2014): The response of root characteristics of Stipa grandis to nitrogen addition in degraded grassland. Acta Prataculturae Sinica, 23: 40–48.
Shen R.C., Xu M., Chi Y.G., Yu S., Wan S.Q. (2014): Soil microbial responses to experimental warming and nitrogen addition in
a temperate steppe of Northern China. Pedosphere, 24: 427–436.
Su J.S., Jing G.H., Jin J.W., Wei L., Liu J., Cheng J.M. (2017): Identifying drivers of root community compositional changes in semiarid grassland on the Loess plateau after long-term grazing exclusion. Ecological Engineering, 99: 13–21. https://doi.org/10.1016/j.ecoleng.2016.11.050
Su Y.Q., Liu H.M., Zheng Z.M., Lin L., Wang X.H. (2016): Effects of N and P addition on soil available nitrogen and pH in a subtropical forest. Chinese Journal of Ecology, 35: 2279–2285.
Ter Braak C.J.F., Smilauer P. (2012): Canoco Reference Manual and User’s Guide: Software for Ordination, version 5.0. Ithaca, Microcomputer Power, 496.
Tu L.H., Peng Y., Chen G., Hu H.L., Xiao Y.L., Hu T.X., Liu L., Tang Y. (2015): Direct and indirect effects of nitrogen additions on fine root decomposition in a subtropical bamboo forest. Plant and Soil, 389: 273–288. https://doi.org/10.1007/s11104-014-2353-9
Vanguelova E.I., Nortcliff S., Moffat A.J., Kennedy F. (2007): Short-term effects of manipulated increase in acid deposition on soil, soil solution chemistry and fine roots in Scots pine (Pinus sylvestris) stand on a podzol. Plant and Soil, 294: 41–54. https://doi.org/10.1007/s11104-007-9225-5
Wang G.L., Fahey T.J., Xue S., Liu F. (2013): Root morphology and architecture respond to N addition in Pinus tabuliformis, west China. Oecologia, 171: 583–590. https://doi.org/10.1007/s00442-012-2441-6
Wang W.N., Wang Y., Hoch G., Wang Z.Q., Gu J.C. (2018): Linkage of root morphology to anatomy with increasing nitrogen availability in six temperate tree species. Plant and Soil, 425: 189–200. https://doi.org/10.1007/s11104-018-3563-3
Wu Y.T., Kwak J.H., Karst J., Ni M.Y., Yan Y.F., Lv X.F., Xu J.M., Chang S.X. (2021): Long-term nitrogen and sulfur deposition increased root-associated pathogen diversity and changed mutualistic fungal diversity in a boreal forest. Soil Biology and Biochemistry, 155: 108163. https://doi.org/10.1016/j.soilbio.2021.108163
Yan G.Y., Chen F., Zhang X., Wang J.Y., Han S.J., Xing Y.J., Wang Q.G. (2017): Spatial and temporal effects of nitrogen addition on root morphology and growth in a boreal forest. Geoderma, 303: 178–187. https://doi.org/10.1016/j.geoderma.2017.05.030
Yue K., Peng Y., Peng C.H., Yang W.Q., Peng X., Wu F.Z. (2016): Stimulation of terrestrial ecosystem carbon storage by nitrogen addition: a meta-analysis. Scientific Reports, 6: 19895. https://doi.org/10.1038/srep19895
Zhang X.L., Wang F.C., Fang X.M., He P., Zhang Y.F., Chen F.S., Wang H.M. (2017): Responses of soil organic carbon and its labile fractions to nitrogen and phosphorus additions in Cunninghamia lanceolata plantations in subtropical China. Chinese Journal of Applied Ecology, 28: 449–455. (In Chinese)
Zheng Z., Bai W.M., Zhang W.H. (2019): Root trait-mediated belowground competition and community composition of a temperate steppe under nitrogen enrichment. Plant and Soil, 437: 341–354. https://doi.org/10.1007/s11104-019-03989-z
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti