Comparison of heat output and CO2 respiration to assess soil microbial activity: a case of ultisol soil X., Cao H., Jiang L., Yuan J., Zheng S. (2018): Comparison of heat output and CO2 respiration to assess soil microbial activity: a case of ultisol soil. Plant Soil Environ., 64: 470-478.
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Glucose-induced microcalorimetry and carbon dioxide (CO2) production are two widely applied methods to assess microbial activity in soil. However, the links among them, microbial communities and soil chemical properties based on large number of soil samples are still not fully understood. Seventy-two soil samples of different land uses were collected from an ultisol soil area in south China. The best correlation between the rate of heat output and the rate of CO2 respiration occurred in 8–16 h reaction (R2 = 0.64), followed by 0–8 h (R2 = 0.50) (P < 0.001). However, the correlations decreased sharply after 16 h. The heat output per biomass unit (QT/MBC) was well correlated with the total phospholipid fatty acids (PLFAs) (R2 = 0.56) and bacterial PLFAs (R2 = 0.53) (P < 0.001). In contrast, these links were not apparent between soil respiratory quotient (qCO2) and the total PLFAs and microbial communities. Redundancy analysis further confirmed that QT/MBC was a more comprehensive indicator to assess soil microbial activity and soil quality than qCO2, showing a good negative correlation to soil organic carbon, total nitrogen (N) and mineral N, and pH. This work is very helpful to better guide the application of calorimetry and CO2 respiration in assessing microbial activity in soils.

Barros N, Feijóo S, Fernández S (2003): Microcalorimetric determination of the cell specific heat rate in soils: relationship with the soil microbial population and biophysic significance. Thermochimica Acta, 406, 161-170
Barros Nieves, Feijóo Sergio, Hansen Lee D. (2011): Calorimetric determination of metabolic heat, CO2 rates and the calorespirometric ratio of soil basal metabolism. Geoderma, 160, 542-547
Barros N., Hansen L.D., Piñeiro V., Pérez-Cruzado C., Villanueva M., Proupín J., Rodríguez-Añón J.A. (2016): Factors influencing the calorespirometric ratios of soil microbial metabolism. Soil Biology and Biochemistry, 92, 221-229
Barros N., Salgado J., Feijóo S. (2007): Calorimetry and soil. Thermochimica Acta, 458, 11-17
Bobille Hélène, Limami Anis M., Robins Richard J., Cukier Caroline, Le Floch Gaëtan, Fustec Joëlle (2016): Evolution of the amino acid fingerprint in the unsterilized rhizosphere of a legume in relation to plant maturity. Soil Biology and Biochemistry, 101, 226-236
Bremner J.M. (1965): Total nitrogen. In: Black C.A., Evans D.D., Ensminger L.E., White J.L., Clark F.E., Dinauer R.C. (eds.): Methods of Soil Analysis. Madison, American Society of Agronomy, Inc., 1149–1178.
Cao Haichuan, Chen Ruirui, Wang Libing, Jiang Lanlan, Yang Fen, Zheng Shixue, Wang Gejiao, Lin Xiangui (2016): Soil pH, total phosphorus, climate and distance are the major factors influencing microbial activity at a regional spatial scale. Scientific Reports, 6, -
Carson P.L. (1980): Recommended potassium test. In: Dahnke W.C. (ed.): Recommended Chemical Soil Test Procedures for the North Central Region. Bulletin 499. Fargo, North Dakota Agricultural Experiment Station.
Chu Haiyan, Lin Xiangui, Fujii Takeshi, Morimoto Sho, Yagi Kazuyuki, Hu Junli, Zhang Jiabao (2007): Soil microbial biomass, dehydrogenase activity, bacterial community structure in response to long-term fertilizer management. Soil Biology and Biochemistry, 39, 2971-2976
Critter Silvana A.M., Freitas Sueli S., Airoldi Claudio (2004): Comparison of microbial activity in some Brazilian soils by microcalorimetric and respirometric methods. Thermochimica Acta, 410, 35-46
Dyckmans Jens, Flessa Heiner, Lipski André, Potthoff Martin, Beese Friedrich (2006): Microbial biomass and activity under oxic and anoxic conditions as affected by nitrate additions. Journal of Plant Nutrition and Soil Science, 169, 108-115
Falkowski P. G., Fenchel T., Delong E. F. (2008): The Microbial Engines That Drive Earth's Biogeochemical Cycles. Science, 320, 1034-1039
Herrmann Anke M., Coucheney Elsa, Nunan Naoise (2014): Isothermal Microcalorimetry Provides New Insight into Terrestrial Carbon Cycling. Environmental Science & Technology, 48, 4344-4352
Herrmann Anke M., Bölscher Tobias (2015): Simultaneous screening of microbial energetics and CO2 respiration in soil samples from different ecosystems. Soil Biology and Biochemistry, 83, 88-92
Jackson M.L. (1958): Soil Chemical Analysis. Englewood Cliffs, Prentice-Hall, Inc., 111–133.
Jenkinson D.S., Ladd J.N. (1981): Microbial biomass in soil: Measurement and turnover. In: Paul E.A., Ladd J.N. (eds.): Soil Biochemistry. Volume 5. New York, Marcel Dekker Inc, 451–471.
Madigan M.T., Martinko J.M., Bender K.S., Buckley D.H., Stahl D.A. (2015): Brock Biology of Microorganisms. 14th Edition. New York, Pearson Education, Inc.
Mebius L.J. (1960): A rapid method for the determination of organic carbon in soil. Analytica Chimica Acta, 22, 120-124
Olsen S.R., Cole C.V., Watanabe F.S., Dean L.A. (1954): Estimation of available phosphorus in soils by extraction with sodium bicarbonate. Washington, USDA Circular No. 939. U.S. Department of Agriculture, 19.
SPARLING G. P. (1983): Estimation of microbial biomass and activity in soil using microcalorimetry. Journal of Soil Science, 34, 381-390
Talbot J. M., Bruns T. D., Taylor J. W., Smith D. P., Branco S., Glassman S. I., Erlandson S., Vilgalys R., Liao H.-L., Smith M. E., Peay K. G. (2014): Endemism and functional convergence across the North American soil mycobiome. Proceedings of the National Academy of Sciences, 111, 6341-6346
Vor Torsten, Dyckmans Jens, Flessa Heiner, Beese Friedrich (2002): Use of microcalorimetry to study microbial activity during the transition from oxic to anoxic conditions. Biology and Fertility of Soils, 36, 66-71
Wadsö I. (2009): Characterization of microbial activity in soil by use of isothermal microcalorimetry. Journal of Thermal Analysis and Calorimetry, 95, 843-850
Wadsö Lars, Hansen Lee D. (2015): Calorespirometry of terrestrial organisms and ecosystems. Methods, 76, 11-19
Wu Yuping, Ding Na, Wang Gang, Xu Jianming, Wu Jianjun, Brookes Philip C. (2009): Effects of different soil weights, storage times and extraction methods on soil phospholipid fatty acid analyses. Geoderma, 150, 171-178
Xu J.B., Feng Y.Z., Barros N., Zhong L.H., Chen R.R., Lin X.G. (2016): Exploring the potential of microcalorimetry to study soil microbial metabolic diversity. Journal of Thermal Analysis and Calorimetry, 127: 1457–1465.
Zheng Shixue, Hu Junli, Chen Ke, Yao Jun, Yu Ziniu, Lin Xiangui (2009): Soil microbial activity measured by microcalorimetry in response to long-term fertilization regimes and available phosphorous on heat evolution. Soil Biology and Biochemistry, 41, 2094-2099
Zheng Shixue, Cao Haichuan, Huang Qiaoyun, Liu Ming, Lin Xiangui, Li Zhongpei (2016): Long-term fertilization of P coupled with N greatly improved microbial activities in a paddy soil ecosystem derived from infertile land. European Journal of Soil Biology, 72, 14-20
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