Antioxidant response to cold stress in two oil plants of the genus Jatropha
Carmelina Spanò, Stefania Bottega, Monica Ruffini Castiglione, Hilda Elizabeth Pedranzanihttps://doi.org/10.17221/182/2017-PSECitation:Spanò C., Bottega S., Ruffini Castiglione M., Pedranzani H.E. (2017): Antioxidant response to cold stress in two oil plants of the genus Jatropha. Plant Soil Environ., 63: 271-276.
Jatropha curcas and J. macrocarpa, suitable for production of biodiesel oil from their seeds, are able to live in arid and semi-arid regions, where most crops cannot survive. J. curcas is characterized by higher oil quality and seed yield, but it is not a good candidate for oil production in arid areas with freezing temperatures, due to its sensitivity to chilling in comparison to J. macrocarpa. In this work, for the first time, the effects of cold stress and different mechanisms activated in these conditions have been studied in the two species. Seedlings were treated with low non-freezing temperatures with or without a previous acclimation period. Water status, pigment content, oxidative stress and antioxidant response were studied in acclimated and non-acclimated plants. The key features that differentiate J. macrocarpa from J. curcas were the ability to accumulate, at low temperatures, high concentrations of pigments and glutathione and significantly higher activities of ascorbate peroxidase. These data could explain the greater resistance to low temperatures of J. macrocarpa. A period of acclimation was not able to improve cold tolerance of J. curcas and this confirms its limited adaptability to arid areas with freezing temperatures.Keywords:
cold acclimation; damage; enzymes; hydrogen peroxide; liquid bio-fuel; reactive oxygen speciesReferences:
Achten W.M.J., Verchot L., Franken Y.J., Mathijs E., Singh V.P., Aerts R., Muys B. (2008): Jatropha bio-diesel production and use. Biomass and Bioenergy, 32, 1063-1084 https://doi.org/10.1016/j.biombioe.2008.03.003Aebi H. (1984): Catalase in vitro. Methods in Enzymology, 105: 121–125.Ao Ping-Xing, Li Zhong-Guang, Gong Ming (2013): Involvement of compatible solutes in chill hardening-induced chilling tolerance in Jatropha curcas seedlings. Acta Physiologiae Plantarum, 35, 3457-3464 https://doi.org/10.1007/s11738-013-1381-zArezki O., Boxus P., Kevers C., Gaspar T. (2001): Changes in peroxidase activity, and level of phenolic compounds during light-induced plantlet regeneration from Eucalyptus camaldulensis Dhen. nodes in vitro. Plant Growth Regulation, 33: 215–219. https://doi.org/10.1023/A:1017579623170Balestri Mirko, Bottega Stefania, Spanò Carmelina (2014): Response of Pteris vittata to different cadmium treatments. Acta Physiologiae Plantarum, 36, 767-775 https://doi.org/10.1007/s11738-013-1454-zBascuñán-Godoy Luisa, Sanhueza Carolina, Cuba Marely, Zuñiga Gustavo E, Corcuera Luis J, Bravo León A (2012): Cold-acclimation limits low temperature induced photoinhibition by promoting a higher photochemical quantum yield and a more effective PSII restoration in darkness in the Antarctic rather than the Andean ecotype of Colobanthus quitensis Kunt Bartl (Cariophyllaceae). BMC Plant Biology, 12, 114- https://doi.org/10.1186/1471-2229-12-114Bradford Marion M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254 https://doi.org/10.1016/0003-2697(76)90527-3Burchett S., Niven S., Fuller M.P. (2006): The effect of cold-acclimation on the water relations and freezing tolerance of Hordeum vulgare L. CryoLetters, 27: 295–303.Francis George, Edinger Raphael, Becker Klaus (2005): A concept for simultaneous wasteland reclamation, fuel production, and socio-economic development in degraded areas in India: Need, potential and perspectives of Jatropha plantations. Natural Resources Forum, 29, 12-24 https://doi.org/10.1111/j.1477-8947.2005.00109.xGossett Dalton R., Millhollon Eddie P., Lucas M. Cran (1994): Antioxidant Response to NaCl Stress in Salt-Tolerant and Salt-Sensitive Cultivars of Cotton. Crop Science, 34, 706- https://doi.org/10.2135/cropsci1994.0011183X003400030020xGusta L.V. (2004): The Effect of Water, Sugars, and Proteins on the Pattern of Ice Nucleation and Propagation in Acclimated and Nonacclimated Canola Leaves. PLANT PHYSIOLOGY, 135, 1642-1653 https://doi.org/10.1104/pp.103.028308Hao W., Arora R., Yadav A.K., Joshee N. (2009): Freezing tolerance and cold acclimation in Guava (Psidium guajva L.). HortScience, 44: 1258–1266.Hassanzadeh M., Ebadi A., Panahyan-e-Kivi M., Eshghi A.G., Jamaati-e-Somarin Sh., Saeidi M., Zabihi-e-Mahmoodabad R. (2009): Evaluation of Drought Stress on Relative Water Content and Chlorophyll Content of Sesame (Sesamum indicum L.) Genotypes at Early Flowering Stage. Research Journal of Environmental Sciences, 3, 345-350 https://doi.org/10.3923/rjes.2009.345.350Hu W. H., Song X. S., Shi K., Xia X. J., Zhou Y. H., Yu J. Q. (2008): Changes in electron transport, superoxide dismutase and ascorbate peroxidase isoenzymes in chloroplasts and mitochondria of cucumber leaves as influenced by chilling. Photosynthetica, 46, 581-588 https://doi.org/10.1007/s11099-008-0098-5Jana Sasadhar, Choudhuri Monojit A. (1982): Glycolate metabolism of three submersed aquatic angiosperms during ageing. Aquatic Botany, 12, 345-354 https://doi.org/10.1016/0304-3770(82)90026-2Jouyban Z., Hasanzade R., Sharafi S. (2013): Chilling stress in plants. International Journal of Agriculture and Crop Sciences, 5: 2961–2968.Kampfenkel K., Vanmontagu M., Inze D. (1995): Extraction and Determination of Ascorbate and Dehydroascorbate from Plant Tissue. Analytical Biochemistry, 225, 165-167 https://doi.org/10.1006/abio.1995.1127Kocsy G., von Ballmoos P., Ruegsegger A., Szalai G., Galiba G., Brunold C. (2001): Increasing the Glutathione Content in a Chilling-Sensitive Maize Genotype Using Safeners Increased Protection against Chilling-Induced Injury. PLANT PHYSIOLOGY, 127, 1147-1156 https://doi.org/10.1104/pp.010107Kocsy Gábor, Szalai Gabriella, Gáliba Gabor (2002): Induction of Glutathione Synthesis and Glutathione Reductase Activity by Abiotic Stresses in Maize and Wheat. The Scientific World JOURNAL, 2, 1699-1705 https://doi.org/10.1100/tsw.2002.812Lichtenthaler H.K. (1987): Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology, 148: 350–382.Lukatkin A.S. (2002): Contribution of oxidative stress to the development of cold-induced damage to leaves of chilling-sensitive plants: 1. Reactive oxygen species formation during plant chilling. Russian Journal of Plant Physiology, 49: 622–627. https://doi.org/10.1023/A:1020232700648Lukatkin Alexander S., Anjum Naser A. (2014): Control of cucumber (Cucumis sativus L.) tolerance to chilling stressâ€”evaluating the role of ascorbic acid and glutathione. Frontiers in Environmental Science, 2, - https://doi.org/10.3389/fenvs.2014.00062Mittler Ron (2002): Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7, 405-410 https://doi.org/10.1016/S1360-1385(02)02312-9Nakano Y., Asada K. (1981): Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22: 867–880.Navari-Izzo F., Meneguzzo S., Loggini B., Vazzana C., Sgherri C. L. M. (1997): The role of the glutathione system during dehydration of Boea hygroscopica. Physiologia Plantarum, 99, 23-30 https://doi.org/10.1111/j.1399-3054.1997.tb03426.xPrasad T. K. (): Evidence for Chilling-Induced Oxidative Stress in Maize Seedlings and a Regulatory Role for Hydrogen Peroxide. THE PLANT CELL ONLINE, 6, 65-74 https://doi.org/10.1105/tpc.6.1.65S. Sanghera Gulzar, H. Wani Shabir, Hussain Wasim, B. Singh N. (2011): Engineering Cold Stress Tolerance in Crop Plants. Current Genomics, 12, 30-43 https://doi.org/10.2174/138920211794520178Spanò Carmelina, Bruno Maria, Bottega Stefania (2013): Calystegia soldanella: dune versus laboratory plants to highlight key adaptive physiological traits. Acta Physiologiae Plantarum, 35, 1329-1336 https://doi.org/10.1007/s11738-012-1173-xTavecchio N., Reinoso H., Castiglione M. Ruffini, Spanò C., Pedranzani H. E. (2016): Anatomical Studies of Two Jatropha Species with Importance for Biodiesel Production. Journal of Agricultural Science, 8, 84- https://doi.org/10.5539/jas.v8n9p84Wang Y.J., Wisniewski M., Meilan R., Cui M.G., Webb R., Fuchigami L. (2005): Overexpression of cytosolic ascorbate peroxidase in tomato confers tolerance to chilling and salt stress. Journal of the American Society for Horticultural Science, 130: 167–173.Wang (2013): RETRACTED: ANALYSIS OF INTERFERING SUBSTANCES IN THE MEASUREMENT OF MALONDIALDEHYDE CONTENT IN PLANT LEAVES. American Journal of Biochemistry and Biotechnology, 9, 235-242 https://doi.org/10.3844/ajbbsp.2013.235.242Wassner D., Larran A., Rondanini D. (2012): Evaluation of Jatropha macrocarpa as an oil crop for biodiesel production in arid lands of the Dry Chaco, Argentina. Journal of Arid Environments, 77, 153-156 https://doi.org/10.1016/j.jaridenv.2011.08.011Zhou Jie, Wang Jian, Shi Kai, Xia Xiao Jian, Zhou Yan Hong, Yu Jing Quan (2012): Hydrogen peroxide is involved in the cold acclimation-induced chilling tolerance of tomato plants. Plant Physiology and Biochemistry, 60, 141-149 https://doi.org/10.1016/j.plaphy.2012.07.010