The influence of genetically modified glyphosate-tolerant maize CC-2 on rhizosphere bacterial communities revealed by MiSeq sequencing

Zhou X.L., Liang J.G., Luan Y., Song X.Y., Zhang Z.G. (2020): The influence of genetically modified glyphosate-tolerant maize CC-2 on rhizosphere bacterial communities revealed by MiSeq sequencing. Plant Soil Environ., 66: 387–394.


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Genetically modified (GM) crops have brought huge economic benefits to mankind, however, at the same time, their safety issues are drawing growing attention. This investigation was conducted to assess whether the long-term cultivation of GM glyphosate resistant maize CC-2 effects bacterial communities in the rhizosphere soil. A 2-year follow-up trial was conducted, and soils were sampled at various plant developmental stages. The bacterial community structure of the rhizosphere soil was analysed by the high-throughput sequencing and compared with the near-isogenic non-GM maize Zheng 58. We showed here that long-term cultivation of CC-2 has no significant effect on the structure and diversity of bacterial communities, while different growth stages had significant effect. These results provided a reliable theoretical basis for the future cultivation and increased commercialisation of CC-2.


Aguirre-von-Wobeser E., Rocha-Estrada J., Shapiro L.R., de la Torre M. (2018): Enrichment of Verrucomicrobia, Actinobacteria and Burkholderiales drives selection of bacterial community from soil by maize roots in a traditional milpa agroecosystem. PLoS One, 13: e0208852.
Bai X., Zeng X., Huang S.Q., Liang J.S., Dong L.Y., Wei Y.N., Li Y., Qu J.J., Wang Z.H. (2019): Marginal impact of cropping BADH transgenic maize BZ-136 on chemical property, enzyme activity, and bacterial community diversity of rhizosphere soil. Plant and Soil, 436: 527–541.
Bhatti A.A., Haq S., Bhat R.A. (2017): Actinomycetes benefaction role in soil and plant health. Microbial Pathogenesis, 111: 458–467.
Bhattacharyya P.N., Jha D.K. (2012): Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology, 28: 1327–1350.
Carvalhais L.C., Dennis P.G., Badri D.V., Tyson G.W., Vivanco J.M., Schenk P.M. (2013): Activation of the jasmonic acid plant defence pathway alters the composition of rhizosphere bacterial communities. PLoS One, 8: e56457.
Chaparro J.M., Badri D.V., Vivanco J.M. (2014): Rhizosphere microbiome assemblage is affected by plant development. The ISME Journal, 8: 790–803.
Dunfield K.E., Germida J.J. (2004): Impact of genetically modified crops on soil- and plant-associated microbial communities. Journal of Environmental Quality, 33: 806–815.
Edgar R.C. (2013): UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods, 10: 996–998.
Fan C.M., Wang B.F., Zhou L., Yin J.Q., Song X.Y. (2018): Effects of transgenic herbicide-resistant corn CC-2 with EPSPS gene cultivation on soil Collembola. Journal of Agro-Environment Science, 37: 1203–1210.
Garbeva P., van Veen J.A., van Elsas J.D. (2004): Microbial diversity in soil: selection of microbial populations by plant and soil type and implications for disease suppressiveness. Annual Review of Phytopathology, 42: 243–270.
García-Salamanca A., Molina-Henares M.A., van Dillewijn P., Solano J., Pizarro-Tobías P., Roca A., Duque E., Ramos J.L. (2013): Bacterial diversity in the rhizosphere of maize and the surrounding carbonate-rich bulk soil. Microbial Biotechnology, 6: 36–44.
Haldar S., Sengupta S. (2015): Plant-microbe cross-talk in the rhizosphere: insight and biotechnological potential. The Open Microbiology Journal, 9: 1–7.
ISAAA (2018): Global Status of Commercialised Biotech/GM Crops in 2018: Biotech Crops Continue to Help Meet the Challenges of Increased Population and Climate Change. ISAAA Brief No. 54. Ithaca, International Service for the Acquisition of Agri-biotech Applications.
IUSS Working Group WRB (2015): World Reference Base for Soil Resources 2014. Update 2015. International Soil Classification System for Naming Soils and Creating Legends for Soil Maps. World Soil Resources Reports No. 106. Rome, Food and Agriculture Organisation, 168.
Kolton M., Harel Y.M., Pasternak Z., Graber E.R., Elad Y., Cytryn E. (2011): Impact of biochar application to soil on the root-associated bacterial community structure of fully developed greenhouse pepper plants. Applied and Environmental Microbiology, 77: 4924–4930.
Kozich J.J., Westcott S.L., Baxter N.T., Highlander S.K., Schloss P.D. (2013): Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Applied and Environmental Microbiology, 79: 5112–5120.
Li X.Z., Rui J.P., Xiong J.B., Li J.B., He Z.L., Zhou J.Z., Yannarell A.C., Mackie R.I. (2014a): Functional potential of soil microbial communities in the maize rhizosphere. PLoS One, 9: e112609.
Li Y.Y., Wen H.Y., Chen L.Q., Yin T.T. (2014b): Succession of bacterial community structure and diversity in soil along a chronosequence of reclamation and re-vegetation on coal mine spoils in China. PLoS One, 9: e115024.
Liang J.G., Jiao Y., Luan Y., Sun S., Wu C.X., Wu H.Y., Zhang M.R., Zhang H.F., Zheng X.B., Zhang Z.G. (2018): A 2-year field trial reveals no significant effects of GM high-methionine soybean on the rhizosphere bacterial communities. World Journal of Microbiology and Biotechnology, 34: 113.
Lu G.H., Tang C.Y., Hua X.M., Cheng J., Wang G.H., Zhu Y.L., Zhang L.Y., Shou H.X., Qi J.L., Yang Y.H. (2018): Effects of an EPSPS-transgenic soybean line ZUTS31 on root-associated bacterial communities during field growth. PLoS One, 13: e0192008.
Mandal A., Sarkar B., Owens G., Thakur J.K., Manna M.C., Niazi N.K., Jayaraman S., Patra A.K. (2020): Impact of genetically modified crops on rhizosphere microorganisms and processes: a review focusing on Bt cotton. Applied Soil Ecology, 148: 103492.
R Development Core Team (2011): R: A language and Environment for Statistical Computing. Vienna, R Foundation for Statistical Computing.
Szoboszlay M., Näther A., Mullins E., Tebbe C.C. (2019): Annual replication is essential in evaluating the response of the soil microbiome to the genetic modification of maize in different biogeographical regions. PLoS One, 14: e0222737.
Shtark O.Y., Shaposhnikov A.I., Kravchenko L.V. (2003): The production of antifungal metabolites by Pseudomonas chlororaphis grown on different nutrient sources. Microbiology, 72: 574–578.
Turrini A., Sbrana C., Giovannetti M. (2015): Belowground environmental effects of transgenic crops: a soil microbial perspective. Research in Microbiology, 166: 121–131.
Wang H.B., Zhang Z.X., Li H., He H.B., Fang C.X., Zhang A.J., Li Q.S., Chen R.S., Guo X.K., Lin H.F., Wu L.K., Lin S., Chen T., Lin R.Y., Peng X.X., Lin W.X. (2011): Characterization of metaproteomics in crop rhizospheric soil. Journal of Proteome Research, 10: 932–940.
Yang Y., Wang N., Guo X.Y., Zhang Y., Ye B.P. (2017): Comparative analysis of bacterial community structure in the rhizosphere of maize by high-throughput pyrosequencing. PLoS One, 12: e0178425.
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