The influence of genetically modified glyphosate-tolerant maize CC-2 on rhizosphere bacterial communities revealed by MiSeq sequencing

https://doi.org/10.17221/216/2020-PSECitation:

Zhou X.L., Liang J.G., Luan Y., Song X.Y., Zhang Z.G. (2020): The influence of genetically modified glyphosate-tolerant maize CC-2 on rhizosphere bacterial communities revealed by MiSeq sequencing. Plant Soil Environ., 66: 387–394.

 

download PDF

Genetically modified (GM) crops have brought huge economic benefits to mankind, however, at the same time, their safety issues are drawing growing attention. This investigation was conducted to assess whether the long-term cultivation of GM glyphosate resistant maize CC-2 effects bacterial communities in the rhizosphere soil. A 2-year follow-up trial was conducted, and soils were sampled at various plant developmental stages. The bacterial community structure of the rhizosphere soil was analysed by the high-throughput sequencing and compared with the near-isogenic non-GM maize Zheng 58. We showed here that long-term cultivation of CC-2 has no significant effect on the structure and diversity of bacterial communities, while different growth stages had significant effect. These results provided a reliable theoretical basis for the future cultivation and increased commercialisation of CC-2.

 

References:
Aguirre-von-Wobeser E., Rocha-Estrada J., Shapiro L.R., de la Torre M. (2018): Enrichment of Verrucomicrobia, Actinobacteria and Burkholderiales drives selection of bacterial community from soil by maize roots in a traditional milpa agroecosystem. PLoS One, 13: e0208852. https://doi.org/10.1371/journal.pone.0208852
 
Bai X., Zeng X., Huang S.Q., Liang J.S., Dong L.Y., Wei Y.N., Li Y., Qu J.J., Wang Z.H. (2019): Marginal impact of cropping BADH transgenic maize BZ-136 on chemical property, enzyme activity, and bacterial community diversity of rhizosphere soil. Plant and Soil, 436: 527–541. https://doi.org/10.1007/s11104-019-03941-1
 
Bhatti A.A., Haq S., Bhat R.A. (2017): Actinomycetes benefaction role in soil and plant health. Microbial Pathogenesis, 111: 458–467. https://doi.org/10.1016/j.micpath.2017.09.036
 
Bhattacharyya P.N., Jha D.K. (2012): Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology, 28: 1327–1350. https://doi.org/10.1007/s11274-011-0979-9
 
Carvalhais L.C., Dennis P.G., Badri D.V., Tyson G.W., Vivanco J.M., Schenk P.M. (2013): Activation of the jasmonic acid plant defence pathway alters the composition of rhizosphere bacterial communities. PLoS One, 8: e56457. https://doi.org/10.1371/journal.pone.0056457
 
Chaparro J.M., Badri D.V., Vivanco J.M. (2014): Rhizosphere microbiome assemblage is affected by plant development. The ISME Journal, 8: 790–803. https://doi.org/10.1038/ismej.2013.196
 
Dunfield K.E., Germida J.J. (2004): Impact of genetically modified crops on soil- and plant-associated microbial communities. Journal of Environmental Quality, 33: 806–815. https://doi.org/10.2134/jeq2004.0806
 
Edgar R.C. (2013): UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods, 10: 996–998. https://doi.org/10.1038/nmeth.2604
 
Fan C.M., Wang B.F., Zhou L., Yin J.Q., Song X.Y. (2018): Effects of transgenic herbicide-resistant corn CC-2 with EPSPS gene cultivation on soil Collembola. Journal of Agro-Environment Science, 37: 1203–1210.
 
Garbeva P., van Veen J.A., van Elsas J.D. (2004): Microbial diversity in soil: selection of microbial populations by plant and soil type and implications for disease suppressiveness. Annual Review of Phytopathology, 42: 243–270. https://doi.org/10.1146/annurev.phyto.42.012604.135455
 
García-Salamanca A., Molina-Henares M.A., van Dillewijn P., Solano J., Pizarro-Tobías P., Roca A., Duque E., Ramos J.L. (2013): Bacterial diversity in the rhizosphere of maize and the surrounding carbonate-rich bulk soil. Microbial Biotechnology, 6: 36–44. https://doi.org/10.1111/j.1751-7915.2012.00358.x
 
Haldar S., Sengupta S. (2015): Plant-microbe cross-talk in the rhizosphere: insight and biotechnological potential. The Open Microbiology Journal, 9: 1–7. https://doi.org/10.2174/1874285801509010001
 
ISAAA (2018): Global Status of Commercialised Biotech/GM Crops in 2018: Biotech Crops Continue to Help Meet the Challenges of Increased Population and Climate Change. ISAAA Brief No. 54. Ithaca, International Service for the Acquisition of Agri-biotech Applications.
 
IUSS Working Group WRB (2015): World Reference Base for Soil Resources 2014. Update 2015. International Soil Classification System for Naming Soils and Creating Legends for Soil Maps. World Soil Resources Reports No. 106. Rome, Food and Agriculture Organisation, 168.
 
Kolton M., Harel Y.M., Pasternak Z., Graber E.R., Elad Y., Cytryn E. (2011): Impact of biochar application to soil on the root-associated bacterial community structure of fully developed greenhouse pepper plants. Applied and Environmental Microbiology, 77: 4924–4930. https://doi.org/10.1128/AEM.00148-11
 
Kozich J.J., Westcott S.L., Baxter N.T., Highlander S.K., Schloss P.D. (2013): Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Applied and Environmental Microbiology, 79: 5112–5120. https://doi.org/10.1128/AEM.01043-13
 
Li X.Z., Rui J.P., Xiong J.B., Li J.B., He Z.L., Zhou J.Z., Yannarell A.C., Mackie R.I. (2014a): Functional potential of soil microbial communities in the maize rhizosphere. PLoS One, 9: e112609. https://doi.org/10.1371/journal.pone.0112609
 
Li Y.Y., Wen H.Y., Chen L.Q., Yin T.T. (2014b): Succession of bacterial community structure and diversity in soil along a chronosequence of reclamation and re-vegetation on coal mine spoils in China. PLoS One, 9: e115024. https://doi.org/10.1371/journal.pone.0115024
 
Liang J.G., Jiao Y., Luan Y., Sun S., Wu C.X., Wu H.Y., Zhang M.R., Zhang H.F., Zheng X.B., Zhang Z.G. (2018): A 2-year field trial reveals no significant effects of GM high-methionine soybean on the rhizosphere bacterial communities. World Journal of Microbiology and Biotechnology, 34: 113. https://doi.org/10.1007/s11274-018-2495-7
 
Lu G.H., Tang C.Y., Hua X.M., Cheng J., Wang G.H., Zhu Y.L., Zhang L.Y., Shou H.X., Qi J.L., Yang Y.H. (2018): Effects of an EPSPS-transgenic soybean line ZUTS31 on root-associated bacterial communities during field growth. PLoS One, 13: e0192008.
 
Mandal A., Sarkar B., Owens G., Thakur J.K., Manna M.C., Niazi N.K., Jayaraman S., Patra A.K. (2020): Impact of genetically modified crops on rhizosphere microorganisms and processes: a review focusing on Bt cotton. Applied Soil Ecology, 148: 103492. https://doi.org/10.1016/j.apsoil.2019.103492
 
R Development Core Team (2011): R: A language and Environment for Statistical Computing. Vienna, R Foundation for Statistical Computing.
 
Szoboszlay M., Näther A., Mullins E., Tebbe C.C. (2019): Annual replication is essential in evaluating the response of the soil microbiome to the genetic modification of maize in different biogeographical regions. PLoS One, 14: e0222737. https://doi.org/10.1371/journal.pone.0222737
 
Shtark O.Y., Shaposhnikov A.I., Kravchenko L.V. (2003): The production of antifungal metabolites by Pseudomonas chlororaphis grown on different nutrient sources. Microbiology, 72: 574–578. https://doi.org/10.1023/A:1026047301457
 
Turrini A., Sbrana C., Giovannetti M. (2015): Belowground environmental effects of transgenic crops: a soil microbial perspective. Research in Microbiology, 166: 121–131. https://doi.org/10.1016/j.resmic.2015.02.006
 
Wang H.B., Zhang Z.X., Li H., He H.B., Fang C.X., Zhang A.J., Li Q.S., Chen R.S., Guo X.K., Lin H.F., Wu L.K., Lin S., Chen T., Lin R.Y., Peng X.X., Lin W.X. (2011): Characterization of metaproteomics in crop rhizospheric soil. Journal of Proteome Research, 10: 932–940. https://doi.org/10.1021/pr100981r
 
Yang Y., Wang N., Guo X.Y., Zhang Y., Ye B.P. (2017): Comparative analysis of bacterial community structure in the rhizosphere of maize by high-throughput pyrosequencing. PLoS One, 12: e0178425. https://doi.org/10.1371/journal.pone.0178425
 
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti