Effect of salinity on seed germination, seedling growth, and inorganic and organic solutes accumulation in sunflower (Helianthus annuus L.)

https://doi.org/10.17221/22/2015-PSECitation:Wu G.-., Jiao Q., Shui Q.-. (2015): Effect of salinity on seed germination, seedling growth, and inorganic and organic solutes accumulation in sunflower (Helianthus annuus L.). Plant Soil Environ., 61: 220-226.
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To investigate the effects of saline stress on seed germination, ion distribution, and organic solutes changes of sunflower (Helianthus annuus L.), in the present study, seeds and 3-week-old seedlings were subjected to a series of external NaCl concentrations (5–200 mmol). The results showed that high salinity (200 mmol) remarkably inhibited the germination of seed and delayed germination time in sunflower. It was found that 25–200 mmol NaCl significantly reduced both stem and leaf dry weight. Concentrations of 100 and 200 mmol also caused a clear reduction in tissues water content. With the increase of NaCl concentration, Na+ concentrations both in root and stem showed the increasing trend, whereas to a lesser degree in root than in stem. In leaf, Na+ concentration remained unchanged when the external concentrations of NaCl were below 100 mmol, while significantly increased by 41-fold when plants were exposed to 200 mmol. By contrast, K+ concentration in root displayed the decreasing trend with the increase of NaCl concentrations. Neither lower (5 and 10 mmol) nor higher (100 and 200 mmol) salinity significantly affected K+ concentration both in stem and leaf, while moderate levels (25 and 50 mmol) significantly enhanced K+ accumulation. High salinity significantly enhanced soluble sugar concentration in stem by 28% and proline in leaf by 166%. It was proposed that sunflower plants adapt to saline stress to some extent through regulating distribution of Na+ and K+, maintaining higher selective absorption capacity for K+ over Na+, and accumulating more osmoprotectants, such as soluble sugar and proline.

References:
Akbarimoghaddam H., Galavi M., Ghanbari A., Panjehkeh N. (2011): Salinity effects on seed germination and seedling growth of bread wheat cultivars. Trakia Journal of Sciences, 9: 43–50.
 
Anuradha C. (2014): Effect of salt stress on seedling growth of sunflower (Helianthus annuus L.). Biotechnology, 3: 15–22.
 
Ashraf M., Harris P.J.C. (2004): Potential biochemical indicators of salinity tolerance in plants. Plant Science, 166, 3-16  https://doi.org/10.1016/j.plantsci.2003.10.024
 
Bates L. S., Waldren R. P., Teare I. D. (1973): Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205-207  https://doi.org/10.1007/BF00018060
 
Farkhondeh R., Nabizadeh E., Jalilnezhad N. (2012): Effect of salinity stress on proline content, membrane stability and water relations in two sugar beet cultivars. International Journal of Agricultural Science, 2: 385–392.
 
Gupta Bhaskar, Huang Bingru (2014): Mechanism of Salinity Tolerance in Plants: Physiological, Biochemical, and Molecular Characterization. International Journal of Genomics, 2014, 1-18  https://doi.org/10.1155/2014/701596
 
JUAN M, RIVERO R, ROMERO L, RUIZ J (2005): Evaluation of some nutritional and biochemical indicators in selecting salt-resistant tomato cultivars. Environmental and Experimental Botany, 54, 193-201  https://doi.org/10.1016/j.envexpbot.2004.07.004
 
Khodarahmpour Z., Ifar M., Motamedi M. (2012): Effects of NaCl salinity on maize (Zea mays L.) at germination and early seedling stage. African Journal of Biotechnology, 11: 298–304.
 
Kronzucker Herbert J., Britto Dev T. (2011): Sodium transport in plants: a critical review. New Phytologist, 189, 54-81  https://doi.org/10.1111/j.1469-8137.2010.03540.x
 
Kronzucker Herbert J., Coskun Devrim, Schulze Lasse M., Wong Jessie R., Britto Dev T. (2013): Sodium as nutrient and toxicant. Plant and Soil, 369, 1-23  https://doi.org/10.1007/s11104-013-1801-2
 
Maathuis Frans J. M., Ahmad Izhar, Patishtan Juan (2014): Regulation of Na+ fluxes in plants. Frontiers in Plant Science, 5, -  https://doi.org/10.3389/fpls.2014.00467
 
Munns Rana, Tester Mark (2008): Mechanisms of Salinity Tolerance. Annual Review of Plant Biology, 59, 651-681  https://doi.org/10.1146/annurev.arplant.59.032607.092911
 
Panuccio M. R., Jacobsen S. E., Akhtar S. S., Muscolo A. (): Effect of saline water on seed germination and early seedling growth of the halophyte quinoa. AoB PLANTS, 6, plu047-plu047  https://doi.org/10.1093/aobpla/plu047
 
Radić Sandra, Peharec Štefanić Petra, Lepeduš Hrvoje, Roje Vibor, Pevalek-Kozlina Branka (2013): Salt tolerance of Centaurea ragusina L. is associated with efficient osmotic adjustment and increased antioxidative capacity. Environmental and Experimental Botany, 87, 39-48  https://doi.org/10.1016/j.envexpbot.2012.11.002
 
Rauf Saeed, Shahzad Muhammad, Teixeira da Silva Jaime A., Noorka Ijaz Rasool (2012): Biomass partitioning and genetic analyses of salinity tolerance in sunflower (Helianthus annuus L.). Journal of Crop Science and Biotechnology, 15, 205-217  https://doi.org/10.1007/s12892-011-0089-0
 
Roy Stuart J, Negrão Sónia, Tester Mark (2014): Salt resistant crop plants. Current Opinion in Biotechnology, 26, 115-124  https://doi.org/10.1016/j.copbio.2013.12.004
 
Rozema J., Flowers T. (2008): ECOLOGY: Crops for a Salinized World. Science, 322, 1478-1480  https://doi.org/10.1126/science.1168572
 
Shabala Sergey, Cuin Tracey A. (2008): Potassium transport and plant salt tolerance. Physiologia Plantarum, 133, 651-669  https://doi.org/10.1111/j.1399-3054.2007.01008.x
 
Talia P., Greizerstein E.J., Hopp H.E., Paniego N., Poggio L., Heinz R.A. (2011): Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes. Biocell, 35: 19–28.
 
Wakeel Abdul, Farooq Muhammad, Qadir Manzoor, Schubert Sven (2011): Potassium Substitution by Sodium in Plants. Critical Reviews in Plant Sciences, 30, 401-413  https://doi.org/10.1080/07352689.2011.587728
 
WANG CHUN-MEI, ZHANG JIN-LIN, LIU XUE-SONG, LI ZHAN, WU GUO-QIANG, CAI JIAN-YI, FLOWERS TIMOTHY J., WANG SUO-MIN (2009): Puccinellia tenuiflora maintains a low Na + level under salinity by limiting unidirectional Na + influx resulting in a high selectivity for K + over Na +. Plant, Cell & Environment, 32, 486-496  https://doi.org/10.1111/j.1365-3040.2009.01942.x
 
Wang S.-M., Zhang J.-L., Flowers T. J. (2007): Low-Affinity Na+ Uptake in the Halophyte Suaeda maritima. PLANT PHYSIOLOGY, 145, 559-571  https://doi.org/10.1104/pp.107.104315
 
Wu Guo-Qiang, Liang Na, Feng Rui-Jun, Zhang Jing-Jing (2013): Evaluation of salinity tolerance in seedlings of sugar beet (Beta vulgaris L.) cultivars using proline, soluble sugars and cation accumulation criteria. Acta Physiologiae Plantarum, 35, 2665-2674  https://doi.org/10.1007/s11738-013-1298-6
 
Xu Sheng, Hu Bing, He Ziyi, Ma Fei, Feng Jianfei, Shen Wenbiao, Yang Jie (2011): Enhancement of Salinity Tolerance during Rice Seed Germination by Presoaking with Hemoglobin. International Journal of Molecular Sciences, 12, 2488-2501  https://doi.org/10.3390/ijms12042488
 
Xue Zhongcai, Zhao Shijie, Gao Huiyuan, Sun Shan (2014): The salt resistance of wild soybean (Glycine soja Sieb. et Zucc. ZYD 03262) under NaCl stress is mainly determined by Na+ distribution in the plant. Acta Physiologiae Plantarum, 36, 61-70  https://doi.org/10.1007/s11738-013-1386-7
 
Yue L.J., Li S.X., Ma Q., Zhou X.R., Wu G.Q., Bao A.K., Zhang J.L., Wang S.M. (2012): NaCl stimulates growth and alleviates water stress in the xerophyte Zygophyllum xanthoxylum. Journal of Arid Environments, 87, 153-160  https://doi.org/10.1016/j.jaridenv.2012.06.002
 
Zhang Z.J., Li H.Z., Zhou W.J., Takeuchi Y., Yoneyama K. (2006): Effect of 5-aminolevulinic acid on development and salt tolerance of potato (Solanum tuberosum L.) microtubers in vitro. Plant Growth Regulation, 49: 27–34.
 
Zhang Jin-Lin, Shi Huazhong (2013): Physiological and molecular mechanisms of plant salt tolerance. Photosynthesis Research, 115, 1-22  https://doi.org/10.1007/s11120-013-9813-6
 
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