High-methionine soybean has no adverse effect on functional diversity of rhizosphere microorganisms  

https://doi.org/10.17221/241/2016-PSECitation:Liang J.G., Xin L.T., Meng F., Sun S., Wu C.X., Wu H.Y., Zhang M.R., Zhang H.F., Zheng X.B., Zhang Z.G. (2016): High-methionine soybean has no adverse effect on functional diversity of rhizosphere microorganisms  . Plant Soil Environ., 62: 441-446.
download PDF

A transgenic high-methionine soybean ZD91 and its non-transgenic parental soybean ZD were investigated to evaluate the potential negative impact of transgene on the microbial community in soil. The Biolog-ECO plate method was used to evaluate the functional diversity and activity of rhizosphere microbial communities at four growth stages of the soybean each year from 2012 to 2013. Results indicated that there was no difference between ZD and ZD91 in the functional diversity of microbial communities in rhizosphere soil. Besides, plant growth stage had stronger effect than cultivar. It was concluded that transgenic soybean ZD91 did not alter the functional diversity of microbial communities in rhizosphere soil.  

Berg Gabriele, Smalla Kornelia (2009): Plant species and soil type cooperatively shape the structure and function of microbial communities in the rhizosphere. FEMS Microbiology Ecology, 68, 1-13  https://doi.org/10.1111/j.1574-6941.2009.00654.x
Choi Keun-Hyung, Dobbs Fred C (1999): Comparison of two kinds of Biolog microplates (GN and ECO) in their ability to distinguish among aquatic microbial communities. Journal of Microbiological Methods, 36, 203-213  https://doi.org/10.1016/S0167-7012(99)00034-2
Donegan K.K., Palm C.J., Fieland V.J., Porteous L.A., Ganio L.M., Schaller D.L., Bucao L.Q., Seidler R.J. (1995): Changes in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endotoxin. Applied Soil Ecology, 2, 111-124  https://doi.org/10.1016/0929-1393(94)00043-7
Gao Nan, Shen Weishou, Lin Xiangui, Shi Weiming (): Influence of transgenic ath-miR399d tomato lines on microbial community and diversity in rhizosphere soil. Soil Science and Plant Nutrition, 61, 259-268  https://doi.org/10.1080/00380768.2014.970116
Garland J.L., Mills A.L. (1991): Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Applied and Environmental Microbiology, 57: 2351–2359.
Giovannetti M., Sbrana C., Turrini A. (2005): The impact of genetically modified crops on soil microbial communities. Rivista Di Biologia, 98: 393–417.
Heuer H., Kroppenstedt R. M., Lottmann J., Berg G., Smalla K. (2002): Effects of T4 Lysozyme Release from Transgenic Potato Roots on Bacterial Rhizosphere Communities Are Negligible Relative to Natural Factors. Applied and Environmental Microbiology, 68, 1325-1335  https://doi.org/10.1128/AEM.68.3.1325-1335.2002
Houlden Ashley, Timms-Wilson Tracey M., Day Martin J., Bailey Mark J. (2008): Influence of plant developmental stage on microbial community structure and activity in the rhizosphere of three field crops. FEMS Microbiology Ecology, 65, 193-201  https://doi.org/10.1111/j.1574-6941.2008.00535.x
Liang Jingang, Meng Fang, Sun Shi, Wu Cunxiang, Wu Haiying, Zhang Mingrong, Zhang Haifeng, Zheng Xiaobo, Song Xinyuan, Zhang Zhengguang, Aroca Ricardo (2015): Community Structure of Arbuscular Mycorrhizal Fungi in Rhizospheric Soil of a Transgenic High-Methionine Soybean and a Near Isogenic Variety. PLOS ONE, 10, e0145001-  https://doi.org/10.1371/journal.pone.0145001
Liang Jingang, Sun Shi, Ji Jun, Wu Haiying, Meng Fang, Zhang Mingrong, Zheng Xiaobo, Wu Cunxiang, Zhang Zhengguang, Balestrini Raffaella (2014): Comparison of the Rhizosphere Bacterial Communities of Zigongdongdou Soybean and a High-Methionine Transgenic Line of This Cultivar. PLoS ONE, 9, e103343-  https://doi.org/10.1371/journal.pone.0103343
Liu Biao, Zeng Qing, Yan Fengming, Xu Haigen, Xu Chongren (2005): Effects of transgenic plants on soil microorganisms. Plant and Soil, 271, 1-13  https://doi.org/10.1007/s11104-004-1610-8
Lv Yueping, Cai Hongsheng, Yu Jianping, Liu Jiali, Liu Qingguo, Guo Changhong (2014): Biosafety assessment of GFP transplastomic tobacco to rhizosphere microbial community. Ecotoxicology, 23, 718-725  https://doi.org/10.1007/s10646-014-1185-y
Mina U (2008): AN APPROACH FOR IMPACT ASSESSMENT OF TRANSGENIC PLANTS ON SOIL ECOSYSTEM. Applied Ecology and Environmental Research, 6, 1-19  https://doi.org/10.15666/aeer/0603_001019
Pu C.L., Liang J.G., Gao J.Y., Wu C.X., Zhang M.R., Zhang Z.G., Cui Z.L., Cao H. (2012): Effects of high producing methionine soybean transferred cystathionine γ-synthase gene on community structure of bacteria in soil. Journal of Nanjing Agricultural University, 35: 8–14.
Shen Ren Fang, Cai Hong, Gong Wan He (2006): Transgenic Bt cotton has no apparent effect on enzymatic activities or functional diversity of microbial communities in rhizosphere soil. Plant and Soil, 285, 149-159  https://doi.org/10.1007/s11104-006-9000-z
Sohn Soo-In, Oh Young-Ju, Kim Byung-Yong, Kweon Soon-Jong, Cho Hyun-Suk, Ryu Tae-Hoon (2015): Effect of genetically modified rice producing resveratrol on the soil microbial communities. Journal of the Korean Society for Applied Biological Chemistry, 58, 795-805  https://doi.org/10.1007/s13765-015-0106-y
Song S., Hou W., Godo I., Wu C., Yu Y., Matityahu I., Hacham Y., Sun S., Han T., Amir R. (): Soybean seeds expressing feedback-insensitive cystathionine  -synthase exhibit a higher content of methionine. Journal of Experimental Botany, 64, 1917-1926  https://doi.org/10.1093/jxb/ert053
Turrini Alessandra, Sbrana Cristiana, Giovannetti Manuela (2015): Belowground environmental effects of transgenic crops: a soil microbial perspective. Research in Microbiology, 166, 121-131  https://doi.org/10.1016/j.resmic.2015.02.006
Wang G.H., Jin J., Chen X.L., Liu J.D., Liu X.B., Herbert S.J. (2007): Biomass and catabolic diversity of microbial communities with long-term restoration, bare fallow and cropping history in Chinese Mollisols. Plant, Soil and Environment, 53: 177–185.
Wu X.-Q., Yuan W.-M., Tian X.-J., Fan B., Fang X., Ye J.-R., Ding X.L. (2012): Specific and functional diversity of endophytic bacteria from pine wood nematode Bursaphelenchus xylophilus with different virulence. International Journal of Biological Sciences, 9: 34–44.
Xie Wenjun, Zhou Jianmin, Wang Huoyan, Chen Xiaoqin, Lu Zhaohua, Yu Junbao, Chen Xiaobing (2009): Short-term effects of copper, cadmium and cypermethrin on dehydrogenase activity and microbial functional diversity in soils after long-term mineral or organic fertilization. Agriculture, Ecosystems & Environment, 129, 450-456  https://doi.org/10.1016/j.agee.2008.10.021
Yang An, Liu Nana, Tian Qiuying, Bai Wenming, Williams Mark, Wang Qibing, Li Linghao, Zhang Wen-Hao (2015): Rhizosphere bacterial communities of dominant steppe plants shift in response to a gradient of simulated nitrogen deposition. Frontiers in Microbiology, 6, -  https://doi.org/10.3389/fmicb.2015.00789
ZAK J, WILLIG M, MOORHEAD D, WILDMAN H (1994): Functional diversity of microbial communities: A quantitative approach. Soil Biology and Biochemistry, 26, 1101-1108  https://doi.org/10.1016/0038-0717(94)90131-7
Zhang Xiaobing, Wang Xujing, Tang Qiaoling, Li Ning, Liu Peilei, Dong Yufeng, Pang Weimin, Yang Jiangtao, Wang Zhixing (2015): Effects of cultivation of OsrHSA transgenic rice on functional diversity of microbial communities in the soil rhizosphere. The Crop Journal, 3, 163-167  https://doi.org/10.1016/j.cj.2014.11.001
Zhao Yongjun, Liu Bo, Zhang Wenguang, Hu Changwei, An Shuqing (2010): Effects of plant and influent C:N:P ratio on microbial diversity in pilot-scale constructed wetlands. Ecological Engineering, 36, 441-449  https://doi.org/10.1016/j.ecoleng.2009.11.011
download PDF

© 2020 Czech Academy of Agricultural Sciences