No significant differences in rhizosphere bacterial communities between Bt maize cultivar IE09S034 and the near-isogenic non-Bt cultivar Zong31 J., Luan Y., Jiao Y., Xin L., Song X., Zheng X., Zhang Z. (2018): No significant differences in rhizosphere bacterial communities between Bt maize cultivar IE09S034 and the near-isogenic non-Bt cultivar Zong31. Plant Soil Environ., 64: 427-434.
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The release of genetically modified (GM) crops has potential to alter the bacterial population within rhizosphere. Here, the potential effect of GM maize cv. IE09S034 containing the Cry1Ie toxin gene from Bacillus thuringiensis (Bt) was investigated under the field conditions. The community composition and the relative abundance of the bacteria in rhizosphere soil were estimated by analysing 16S rRNA PCR amplicons. Our results indicated that Bt maize IE09S034 has no significant effects on the rhizosphere bacterial community. Instead, it was found that factors such as plant growth stage and year have a stronger effect on the bacterial population dynamics. Our findings therefore provide reliable evidence supporting the potential commercial cultivation of the cv. IE09S034.

Caporaso J. G., Lauber C. L., Walters W. A., Berg-Lyons D., Lozupone C. A., Turnbaugh P. J., Fierer N., Knight R. (2011): Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proceedings of the National Academy of Sciences, 108, 4516-4522
Cotta Simone Raposo, Dias Armando Cavalcante Franco, Marriel Ivanildo Evódio, Andreote Fernando Dini, Seldin Lucy, van Elsas Jan Dirk, Schottel J. L. (2014): Different Effects of Transgenic Maize and Nontransgenic Maize on Nitrogen-Transforming Archaea and Bacteria in Tropical Soils. Applied and Environmental Microbiology, 80, 6437-6445
Cui K., Shoemaker S.P. (2018): Public perception of genetically-modified (GM) food: A Nationwide Chinese consumer study. Science of Food, 2: 10.
DeBruyn Jennifer M., Bevard David A., Essington Michael E., McKnight Julie Y., Schaeffer Sean M., Baxter Holly L., Mazarei Mitra, Mann David G. J., Dixon Richard A., Chen Fang, Zhuo Chunliu, Wang Zeng-Yu, Stewart Charles Neal (2017): Field-grown transgenic switchgrass ( Panicum virgatum L.) with altered lignin does not affect soil chemistry, microbiology, and carbon storage potential. GCB Bioenergy, 9, 1100-1109
Edgar Robert C., Haas Brian J., Clemente Jose C., Quince Christopher, Knight Rob (2011): UCHIME improves sensitivity and speed of chimera detection. Bioinformatics, 27, 2194-2200
Fang M., Kremer R. J., Motavalli P. P., Davis G. (2005): Bacterial Diversity in Rhizospheres of Nontransgenic and Transgenic Corn. Applied and Environmental Microbiology, 71, 4132-4136
Filion M. (2008): Do transgenic plants affect rhizobacteria populations? Microbial Biotechnology, 1: 463–475.
Guan Z.J., Lu S.B., Huo Y.L., Guan Z.P., Liu B., Wei W. (2016): Do genetically modified plants affect adversely on soil microbial communities? Agriculture, Ecosystems and Environment, 235: 289–305.
Guo Jingfei, He Kanglai, Hellmich Richard L., Bai Shuxiong, Zhang Tiantao, Liu Yunjun, Ahmed Tofael, Wang Zhenying (2016): Field trials to evaluate the effects of transgenic cry1Ie maize on the community characteristics of arthropod natural enemies. Scientific Reports, 6, -
Li Yonghui, Liu Yanmin, Yin Xinming, Romeis Jörg, Song Xinyuan, Chen Xiuping, Geng Lili, Peng Yufa, Li Yunhe (2017): Consumption of Bt Maize Pollen Containing Cry1Ie Does Not Negatively Affect Propylea japonica (Thunberg) (Coleoptera: Coccinellidae). Toxins, 9, 108-
Liang J.G., Sun S., Ji J., Wu H.Y., Meng F., Zhang M.R., Zheng X.B., Wu C.X., Zhang Z.G. (2014): Comparison of the rhizosphere bacterial communities of Zigongdongdou soybean and a high-methionine transgenic line of this cultivar. PLoS One, 9: e103343.
Liang J.G., Meng F., Sun S., Wu C.X., Wu H.Y., Zhang M.R., Zhang H.F., Zheng X.B., Song X.Y., Zhang Z.G. (2015): Community structure of arbuscular mycorrhizal fungi in rhizospheric soil of a transgenic high-methionine soybean and a near isogenic variety. PLoS One, 10: e0145001.
JG Liang, LT Xin, Meng F., Sun S., CX Wu, HY Wu, MR Zhang, HF Zhang, XB Zheng, ZG Zhang (2016): High-methionine soybean has no adverse effect on functional diversity of rhizosphere microorganisms  . Plant, Soil and Environment, 62, 441-446
Liang J.G., Meng F., Zhang Z.G. (2017): Effect of a high-methionine transgenic soybean (Glycine max) on soil organic elements and enzyme activities in the rhizosphere. Journal of Biosafety, 26: 301–306.
Jingang Liang, Ying Luan, Yue Jiao, Shi Sun, Cunxiang Wu, Haiying Wu, Mingrong Zhang, Haifeng Zhang, Xiaobo Zheng, Zhengguang Zhang (2018): High-methionine soybean has no significant effect on nitrogen-transforming bacteria in rhizosphere soil. Plant, Soil and Environment, 64, 108-113
Liu X.Y., Wang B.F., Zhou L., Feng S.D., Song X.Y. (2016): Effects of Cry1Ie on soil macrofauna diversity in transgenic corn IE09S034 fields. Crops, 1: 62–68.
Lu G.H., Hua X.M., Liang L., Wen Z.L., Du M.H., Meng F.F., Pang Y.J., Qi J.L., Tang C.Y., Yang Y.H. (2018a): Identification of major rhizobacterial taxa affected by a glyphosate-tolerant soybean line via shotgun metagenomic approach. Genes, 9: E214.
Lu G.H., Tang C.Y., Hua X.M., Cheng J., Wang G.H., Zhu Y.L., Zhang L.Y., Shou H.X., Qi J.L., Yang Y.H. (2018b): Effects of an EPSPS-transgenic soybean line ZUTS31 on root-associated bacterial communities during field growth. PLoS One, 13: e0192008.
Lundin D., Severin I., Logue J.B., Ostman O., Andersson A.F., Lindström E.S. (2012): Which sequencing depth is sufficient to describe patterns in bacterial α- and β-diversity? Environmental Microbiology Reports, 4: 367–372.
Lynch Michael D. J., Neufeld Josh D. (2015): Ecology and exploration of the rare biosphere. Nature Reviews Microbiology, 13, 217-229
Miethling-Graff Rona, Dockhorn Susanne, Tebbe Christoph C. (2010): Release of the recombinant Cry3Bb1 protein of Bt maize MON88017 into field soil and detection of effects on the diversity of rhizosphere bacteria. European Journal of Soil Biology, 46, 41-48
Peiffer J. A., Spor A., Koren O., Jin Z., Tringe S. G., Dangl J. L., Buckler E. S., Ley R. E. (2013): Diversity and heritability of the maize rhizosphere microbiome under field conditions. Proceedings of the National Academy of Sciences, 110, 6548-6553
Penton C. Ryan, Gupta Vadakattu V. S. R., Yu Julian, Tiedje James M. (2016): Size Matters: Assessing Optimum Soil Sample Size for Fungal and Bacterial Community Structure Analyses Using High Throughput Sequencing of rRNA Gene Amplicons. Frontiers in Microbiology, 7, -
Schmalenberger Achim, Tebbe Christoph C. (2002): Bacterial community composition in the rhizosphere of a transgenic, herbicide-resistant maize (Zea mays) and comparison to its non-transgenic cultivar Bosphore. FEMS Microbiology Ecology, 40, 29-37
Schloss P. D., Westcott S. L., Ryabin T., Hall J. R., Hartmann M., Hollister E. B., Lesniewski R. A., Oakley B. B., Parks D. H., Robinson C. J., Sahl J. W., Stres B., Thallinger G. G., Van Horn D. J., Weber C. F. (2009): Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities. Applied and Environmental Microbiology, 75, 7537-7541
Singh A.K., Dubey S.K. (2017): 8 – Transgenic plants and soil microbes. Current Developments in Biotechnology and Bioengineering: Crop Modification, Nutrition, and Food Production, 163–185.
Song F., Zhang J., Gu A., Wu Y., Han L., He K., Chen Z., Yao J., Hu Y., Li G., Huang D. (2003): Identification of cry1I-Type Genes from Bacillus thuringiensis Strains and Characterization of a Novel cry1I-Type Gene. Applied and Environmental Microbiology, 69, 5207-5211
Tundup Phuntsog, Namgail D., Sangdup Safal Rigzin, Gupta Vikas, Namgail Deldan (2017): Genetically Modified Crops and Soil Ecology: A Critical Review. International Journal of Current Microbiology and Applied Sciences, 6, 3962-3969
Turrini Alessandra, Sbrana Cristiana, Giovannetti Manuela (2015): Belowground environmental effects of transgenic crops: a soil microbial perspective. Research in Microbiology, 166, 121-131
Wagner Josef, Coupland Paul, Browne Hilary P., Lawley Trevor D., Francis Suzanna C., Parkhill Julian (2016): Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification. BMC Microbiology, 16, -
Yang Caiyun, Li Yi, Zhou Benjamin, Zhou Yanyan, Zheng Wei, Tian Yun, Van Nostrand Joy D., Wu Liyou, He Zhili, Zhou Jizhong, Zheng Tianling (2015): Illumina sequencing-based analysis of free-living bacterial community dynamics during an Akashiwo sanguine bloom in Xiamen sea, China. Scientific Reports, 5, -
Zhan A.B., Xiong W., He S., MacIsaac H.J. (2014): Influence of artifact removal on rare species recovery in natural complex communities using high-throughput sequencing. PLoS One, 9: e96928.
Zhang Yan-Jun, Xie Ming, Wu Gang, Peng De-Liang, Yu Wen-Bin (2015): A 3-year field investigation of impacts of Monsanto’s transgenic Bt-cotton NC 33B on rhizosphere microbial communities in northern China. Applied Soil Ecology, 89, 18-24
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