Effect of organic fertilisers on glomalin content and soil organic matter quality


Balík J., Sedlář O., Kulhánek M., Černý J., Smatanová M., Suran P. (2020): Effect of organic fertilisers on glomalin content and soil organic matter quality. Plant Soil Environ., 66: 590–597.


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Glomalin is one of the factors with an important role at forming and stabilising soil aggregates. Long-term stationary experiments were carried out to observe the influence of various fertilisation treatments on the content of glomalin in topsoil. The content of easily extractable glomalin (EEG) and total glomalin (TG) were determined. Moreover, glomalin was also determined by using the near-infrared reflectance spectroscopy (GNIRS). Both mineral and organic fertilisation significantly increased the content of glomalin compared to the unfertilised control. However, observed differences among individual fertilisation treatments were not significant. A significant correlation was determined between the content of EEG, TG, GNIRS, and the content of humic substances as well as humic acids. Both methods used (EEG, TG) can equally reflect soil organic matter quality. A significant correlation was also recorded between the GNIRS and extraction methods (EEG, TG).


Alguacil M.M., Lumini E., Roldán A., Salinas-García J.R., Bonfante P., Bianciotto V. (2008): The impact of tillage practices on arbuscular mycorrhizal fungal diversity in subtropical crops. Ecological Applications, 15: 527–536. https://doi.org/10.1890/07-0521.1
Bedini S., Avio L., Sbrana C., Turrini A., Migliorini P., Vazzana C., Giovanneti M. (2013): Mycorrhizal activity and diversity in a long-term organic Mediterranean agroecosystem. Biology and Fertility of Soils, 49: 781–790. https://doi.org/10.1007/s00374-012-0770-6
Turgay O.C., Buchan D., Moeskops B., de Gusseme B., Ortas I., de Neve S. (2015): Changes in soil ergosterol content, glomalin-related soil protein, and phospholipid fatty acid profile as affected by long-term organic and chemical fertilization practices in Mediterranean Turkey. Arid Land Research and Management, 29: 180–198. https://doi.org/10.1080/15324982.2014.944246
Curaqueo G., Barea J.M., Acevedo E., Rubio R., Cornejo P., Borie F. (2011): Effects of different tillage system on arbuscular mycorrhizal fungal propagules and physical properties in a Mediterranean agroecosystem in central Chile. Soil and Tillage Research, 113: 11–18. https://doi.org/10.1016/j.still.2011.02.004
Dai J., Hu J.L., Lin X.G., Yang A., Wang R., Zhang J.B., Wong M.H. (2013): Arbuscular mycorrhizal fungal diversity, external mycelium length, and glomalin-related soil protein content in response to long-term fertilizer management. Journal of Soils and Sediments, 13: 1–11. https://doi.org/10.1007/s11368-012-0576-z
Driver J.D., Holben W.E., Rillig M.C. (2005): Characterization of glomalin as hyphal wall component of arbuscular mycorrhizal fungi. Soil Biology and Biochemistry, 37: 101–106. https://doi.org/10.1016/j.soilbio.2004.06.011
Emran M., Gispert M., Pardini G. (2012): Patterns of soil organic carbon, glomalin and structural stability in abandoned Mediterranean terraced lands. European Journal of Soil Science, 63: 637–649. https://doi.org/10.1111/j.1365-2389.2012.01493.x
Gadkar V., Rillig M.C. (2006): The arbuscular mycorrhizal fungal protein glomalin is a putative homolog of heat shock protein 60. FEMS Microbiology Letters, 263: 93–101. https://doi.org/10.1111/j.1574-6968.2006.00412.x
Galazka A., Gawryjolek K., Grzadziel J., Ksiezak J. (2017): Effect of different agricultural management practices on soil biological parameters including glomalin fraction. Plant, Soil and Environment, 63: 300–306. https://doi.org/10.17221/207/2017-PSE
Gispert M., Emran M., Pardini G., Doni S., Ceccanti B. (2013): The impact of land management and abandonment on soil enzymatic activity, glomalin content and aggregate stability. Geoderma, 202–203: 51–61. https://doi.org/10.1016/j.geoderma.2013.03.012
Harner M.J., Ramsay P.W., Rillig M.C. (2004): Protein accumulation and distribution in floodplain soils and river foam. Ecology Letters, 7: 829–836. https://doi.org/10.1111/j.1461-0248.2004.00638.x
Jastrow J.D. (1996): Soil aggregate formation and the accrual of particulate and mineral-associated organic matter. Soil Biology and Biochemistry, 28: 665–676. https://doi.org/10.1016/0038-0717(95)00159-X
Kononova M.M. (1963): Soil Organic Matter: Nature, Properties and Methods of Study. Moscow, AN SSSR. (In Russian)
Luna L., Miralles I., Andrenelli M.C., Gispert M., Pellergini S., Vignozzi N., Solé-Benet A. (2016): Restoration techniques affect soil organic carbon, glomalin and aggregate stability in degraded soils of a semiarid Mediterranean region. Catena, 143: 256–264. https://doi.org/10.1016/j.catena.2016.04.013
Nie J., Zhou J.M., Wang H.Y., Chen X.Q., Du C.W. (2007): Effect of long-term rice straw return on soil glomalin, carbon and nitrogen. Pedosphere, 17: 295–302. https://doi.org/10.1016/S1002-0160(07)60036-8
Nichols K.A., Wright S.F. (2006): Carbon and nitrogen in operationally defined soil organic matter pools. Biology and Fertility of Soils, 43: 215–220. https://doi.org/10.1007/s00374-006-0097-2
Rillig M.C. (2004): Arbuscular mycorrhizae, glomalin, and soil aggregation. Canadian Journal of Soil Science, 84: 355–363. https://doi.org/10.4141/S04-003
Sandeep S., Manjaiah K.M., Pal S., Singh A.K. (2016): Soil carbon fractions under maize-wheat system: effect of tillage and nutrient management. Environmental Monitoring and Assessment, 188: 14. https://doi.org/10.1007/s10661-015-4995-3
Schindler F.V., Mercer E.J., Rice J.A. (2007): Chemical characteristics of glomalin-related soil protein (GRSP) extracted from soils of varying organic matter content. Soil Biology and Biochemistry, 39: 320–329. https://doi.org/10.1016/j.soilbio.2006.08.017
Singh P.K., Singh M., Tripathi B.N. (2013): Glomalin: an arbuscular and mycorrhizal fungal soil protein. Protoplasma, 250: 663–669. https://doi.org/10.1007/s00709-012-0453-z
Six J., Elliot E.T., Paustian K. (2000): Soil macroaggregate turnover and microaggregate formation: a mechanism for C sequestration under no-tillage agriculture. Soil Biology and Biochemistry, 32: 2099–2103. https://doi.org/10.1016/S0038-0717(00)00179-6
Smatanová M., Komprsová I. (2019): Determination of organic carbon in soil, interpretation of results. In: Proceedings of the 25th International Conference on Reasonable Use of Fertilizers; Dedicated to the Importance and the Role of Organic Matter in Soil. Prague, Czech University of Life Sciences, 73–91.
Steinberg P.D., Rillig M.C. (2003): Differential decomposition of arbuscular mycorrhizal fungal hyphae and glomalin. Soil Biology and Biochemistry, 35: 191–194. https://doi.org/10.1016/S0038-0717(02)00249-3
Šarapatka B., Alvarado-Solano D.P., Čižmár D. (2019): Can glomalin content be used as an indicator for erosion damage to soil and related changes in organic matter characteristics and nutrients? Catena, 181: 104078. https://doi.org/10.1016/j.catena.2019.104078
Wang Q., Wang W.J., He X.Y., Zhang W.T., Song K.S., Han S.J. (2015): Role and variation of the amount and composition of glomalin in soil properties in farmland and adjacent plantations with reference to a primary forest in North-Eastern China. PLOS One, 10: e0139623. https://doi.org/10.1371/journal.pone.0139623
Wilson G.W.T., Rice C.W., Rillig M.C., Springer A., Hartnett D.C. (2009): Soil aggregation and carbon sequestration are tightly correlated with the abundance of arbuscular mycorrhizal fungi: results from long-term field experiments. Ecology Letters, 12: 452–461. https://doi.org/10.1111/j.1461-0248.2009.01303.x
Wojewódzki P., Cieścińska B. (2012): Effect of crop rotation and long term fertilization on the carbon and glomalin content in the soil. Journal of Central European Agriculture, 13: 814–821. https://doi.org/10.5513/JCEA01/13.4.1134
Wright S.F., Anderson R.L. (2000): Aggregate stability and glomalin in alternative crop rotations for the central Great Plains. Biology and Fertility of Soils, 31: 249–253. https://doi.org/10.1007/s003740050653
Wright S.F., Upadhyaya A. (1996): Extraction of an abundant and unusual protein from soil and comparison with hyphal protein of arbuscular mycorrhizal fungi. Soil Science, 161: 575–586. https://doi.org/10.1097/00010694-199609000-00003
Wright S.F., Upadhyaya A. (1998): A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi. Plant and Soil, 198: 97–107. https://doi.org/10.1023/A:1004347701584
Wuest S.B., Caesar-TonThat T.C., Wright S.F., Williams J.D. (2005): Organic matter addition N, and residue burning effects on infiltration, biological, and physical properties of an intensively tilled silt-loam soil. Soil and Tillage Research, 84: 154–167. https://doi.org/10.1016/j.still.2004.11.008
Xie H.T., Li J.W., Zhang B., Wang L.F., Wang J.K., He H.B., Zhang X.D. (2015): Long-term manure amendments reduced soil aggregate stability via redistribution of the glomalin-related soil protein in macroaggregates. Scientific Reports, 5: 14687. https://doi.org/10.1038/srep14687
Zhang X., Wu X., Zhang S.X., Xing Y.H., Wang R., Liang W.J. (2014): Organic amendment effects on aggregate-associated organic C, microbial biomass C and glomalin in agricultural soils. Catena, 123: 188–194. https://doi.org/10.1016/j.catena.2014.08.011
Zbíral J., Čižmár D., Malý S., Obdržálková E. (2017): Determination of glomalin in agriculture and forest soils by near-infrared spectroscopy. Plant, Soil and Environment, 63: 226–230. https://doi.org/10.17221/181/2017-PSE
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