Salt acclimation induced salt tolerance in wild-type and chlorophyl b-deficient mutant wheat

Zuo Z.Y., Ye F., Wang Z.S., Li S.X., Li H., Guo J.H., Mao H.P., Zhu X.C., Li X.N. (2021): Salt acclimation induced salt tolerance in wild-type and chlorophyl b-deficient mutant wheat. Plant Soil Environ., 67: 26–32.


download PDF

Salt acclimation can promote the tolerance of wheat plants to the subsequent salt stress, which may be related to the responses of the photosynthetic apparatus. The chlorophyl (Chl) b-deficient mutant wheat ANK 32B and its wild type (WT) were firstly saltly acclimated with 30 mmol NaCl for 12 days, then subsequently subjected to 6-day salt stress (500 mmol NaCl). The ANK 32B mutant plants had lower Chl b concentration, which was manifested in the lower total Chl concentration, higher ratio of Chl a/b and in reduced photosynthetic activity (Pn). The effect of salt acclimation was manifested mainly after salt stress. Compared to non-acclimated plants, the salt acclimation increased the leaf water potential, osmotic potential (Ψo) and K concentration, while decreased the amount of Na+ and H2O2 in WT and ANK 32B under salt stress, except for Ψo in ANK 32B. In addition, the salt acclimation enhanced the APX (ascorbate peroxidase) activity by 10.55% and 33.69% in WT and ANK 32B under salt stress, respectively. Compared to the genotypes, under salt stress, the Ψo, Fv/Fm, Pn and gs of mutant plants were 5.60, 17.62, 46.73 and 26.41% lower than that of WT, respectively. These results indicated that although the salt acclimation could alleviate the negative consequences of salt stress, it is mainly manifested in the WT, and the ANK 32B plants had lower salt tolerance than WT plants, suggesting that lower Chl b concentration has a negative effect on the salt acclimation induced salt tolerance in wheat.


Alscher R.G., Donahue J.L., Cramer C.L. (1997): Reactive oxygen species and antioxidants: relationships in green cells. Physiologia Plantarum, 100: 224–233.
Arnon D.I. (1949): Copper enzymes in isolated chloroplasts. Polyphenoloxidase in β vulgaris. Plant Physiology, 24: 1–15.
Bargaz A., Nassar R.M.A., Rady M.M., Gaballah M.S., Thompson S.M., Brestic M., Schmidhalter U., Abdelhamid M.T. (2016): Improved salinity tolerance by phosphorus fertilizer in two Phaseolus vulgaris recombinant inbred lines contrasting in their P-efficiency. Journal of Agronomy and Crop Science, 202: 497–507.
Brestic M., Zivcak M., Kunderlikova K., Allakhverdiev S.I. (2016): High temperature specifically affects the photoprotective responses of chlorophyll b-deficient wheat mutant lines. Photosynthesis Research, 130: 251–266.
Brestic M., Zivcak M., Kunderlikova K., Sytar O., Shao H.B., Kalaji H.M., Allakhverdiev S.I. (2015): Low PSI content limits the photoprotection of PSI and PSII in early growth stages of chlorophyll b-deficient wheat mutant lines. Photosynthesis Research, 125: 151–166.
Dinneny J.R. (2014): Traversing organizational scales in plant salt-stress responses. Current Opinion in Plant Biology, 23: 70–75.
Djanaguiraman M., Sheeba J.A., Shanker A.K., Devi D.D., Bangarusamy U. (2006): Rice can acclimate to lethal level of salinity by pretreatment with sublethal level of salinity through osmotic adjustment. Plant and Soil, 284: 363–373.
El-Hendawy S.E., Hassan W.M., Al-Suhaibani N.A., Refay Y., Abdella K.A. (2017): Comparative performance of multivariable agro-physiological parameters for detecting salt tolerance of wheat cultivars under simulated saline field growing conditions. Frontiers Plant Science, 8: 435.
Faseela P., Sinisha A.K., Brestic M., Puthur J.T. (2020): Chlorophyll a fluorescence parameters as indicators of a particular abiotic stress in rice. Photosynthetica, 58: 293–300.
Janda T., Darko É., Shehata S., Kovács V., Pál M., Szalai G. (2016): Salt acclimation processes in wheat. Plant Physiology and Biochemistry, 101: 68–75.
Kalaji H.M., Schansker G., Brestic M., Bussotti F., Calatayud A., Ferroni L., Goltsev V., Guidi L., Jajoo A., Li P., Losciale P., Mishra V.K., Misra A.N., Nebauer S.G., Pancaldi S., Penella C., Pollastrini M., Suresh K., Tambussi E., Yanniccari M., Zivcak M., Cetner M.D., Samborska I.A., Stirbet A., Olsovska K., Kunderlikova K., Shelonzek H., Rusinowski S., Baba W. (2017): Frequently asked questions about chlorophyll fluorescence, the sequel. Photosynthesis Research, 132: 13–66.
Keunen E., Peshev D., Vangronsveld J., Van Den Ende W., Cuypers A. (2013): Plant sugars are crucial players in the oxidative challenge during abiotic stress: extending the traditional concept. Plant, Cell and Environment, 36: 1242–1255.
Li S.L., Li X.N., Wei Z.H., Liu F.L. (2020): ABA-mediated modulation of elevated CO2 on stomatal response to drought. Current Opinion in Plant Biology, 56: 174–180.
Li X.N., Jiang H.D., Liu F.L., Cai J.B., Dai T.B., Cao W.X., Jiang D. (2013): Induction of chilling tolerance in wheat during germination by pre-soaking seed with nitric oxide and gibberellin. Plant Growth Regulation, 71: 31–40.
Li X.N., Cai J., Liu F.L., Dai T.B., Cao W.X., Jiang D. (2014): Cold priming drives the sub-cellular antioxidant systems to protect photosynthetic electron transport against subsequent low temperature stress in winter wheat. Plant Physiology and Biochemistry, 82: 34–43.
Mandhania S., Madan S., Sawhney V. (2006): Antioxidant defense mechanism under salt stress in wheat seedlings. Biologia Plantarum, 50: 227–231.
Munns R. (2005): Genes and salt tolerance: bringing them together. New Phytologist, 167: 645–663.
Munns R., Tester M. (2008): Mechanisms of salinity tolerance. Annual Review Plant Biology, 59: 651–681.
Müller M., Kunz H.-H., Schroeder J.I., Kemp G., Young H.S., Neuhaus H.E. (2014): Decreased capacity for sodium export out of Arabidopsis chloroplasts impairs salt tolerance, photosynthesis and plant performance. Plant Journal, 78: 646–658.
Pandolfi C., Azzarello E., Mancuso S., Shabala S. (2016): Acclimation improves salt stress tolerance in Zea mays plants. Journal of Plant Physiology, 201: 1–8.
Parida A.K., Das A.B. (2005): Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60: 324–349.
Rahnama A., James R.A., Poustini K., Munns R. (2010): Stomatal conductance as a screen for osmotic stress tolerance in durum wheat growing in saline soil. Functional Plant Biology, 37: 255–263.
Santangeli M., Capo C., Beninati S., Pietrini F., Forni C. (2019): Gradual exposure to salinity improves tolerance to salt stress in rapeseed (Brassica napus L.). Water, 11: 1667.
Sun Z.W., Ren L.K., Fan J.W., Li Q., Wang K.J., Guo M.M., Wang L., Li J., Zhang G.X., Yang Z.Y., Chen F., Li X.N. (2016): Salt response of photosynthetic electron transport system in wheat cultivars with contrasting tolerance. Plant, Soil and Environment, 62: 515–521.
Wang Z., Li X., Zhu X., Liu S., Song F., Liu F., Wang Y., Qi X., Wang F., Zuo Z., Duan P., Yang A., Cai J., Jiang D. (2017): Salt acclimation induced salt tolerance is enhanced by abscisic acid priming in wheat. Plant, Soil and Environment, 63: 307–314.
Zhao C.Z., Zhang H., Song C.P., Zhu J.-K., Shabala S. (2020): Mechanisms of plant responses and adaptation to soil salinity. The Innovation, 1: 100017.
Zivcak M., Brestic M., Botyanszka L., Chen Y.E., Allakhverdiev S.I. (2019): Phenotyping of isogenic chlorophyll-less bread and durum wheat mutant lines in relation to photoprotection and photosynthetic capacity. Photosynthesis Research, 139: 239–251.
Zivcak M., Brückova K., Sytar O., Brestic M., Olsovska K., Allakhverdiev S.I. (2017): Lettuce flavonoids screening and phenotyping by chlorophyll fluorescence excitation ratio. Planta, 245: 1215–1229.
Zuo Z.Y., Gao J.H., Xin C.Y., Liu S.Q., Mao H.P., Wang Y.J., Li X.N. (2019): Salt acclimation induced salt tolerance in wild-type and abscisic acid-deficient mutant barley. Plant, Soil and Environment, 65: 516–521.
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti