Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy

https://doi.org/10.17221/430/2021-PSECitation:

Miljaković D., Marinković J., Ignjatov M., Milošević D., Nikolić Z., Tintor B., Đukić V. (2022): Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy. Plant Soil Environ., 68: 59–64.

 

download PDF

The competitiveness of Bradyrhizobium japonicum inoculation strain against indigenous rhizobia was examined in a soil pot experiment. The effect of inoculation strain was evaluated under different soil conditions: with or without previously grown soybean and applied commercial inoculant. Molecular identification of inoculation strain and investigated rhizobial isolates, obtained from nodules representing inoculated treatments, was performed based on 16S rDNA and enterobacterial repetitive intergenic consensus (ERIC) sequencing. Inoculation strain showed a significant effect on the investigated parameters in both soils. Higher nodule occupancy (45% vs. 18%), nodule number (111% vs. 5%), nodule dry weight (49% vs. 9%), shoot length (15% vs. 7%), root length (31% vs. 13%), shoot dry weight (34% vs. 11%), shoot nitrogen content (27% vs. 2%), and nodule nitrogen content (9% vs. 5%) was detected in soil without previously grown soybean and applied commercial inoculant. Soil had a significant effect on the shoot, root and nodule nitrogen content, while interaction of experimental factors significantly altered dry weight and nitrogen content of shoots, roots and nodules, as well as number of nodules. Nodulation parameters were significantly related with shoot dry weight, shoot and nodule nitrogen content. Symbiotic performance of inoculation strains in the field could be improved through co-selection for their competitiveness and effectiveness.

 

References:
De Bruijn F.J. (1992): Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria. Applied and Environmental Microbiology, 58: 2180–2187. https://doi.org/10.1128/aem.58.7.2180-2187.1992
 
DiCenzo G.C., Zamani M., Checcucci A., Fondi M., Griffitts J.S., Finan T.M., Mengoni A. (2019): Multidisciplinary approaches for studying rhizobium-legume symbioses. Canadian Journal of Microbiology, 65: 1–33. https://doi.org/10.1139/cjm-2018-0377
 
Griebsch A., Matschiavelli N., Lewandowska S., Schmidtke K. (2020): Presence of Bradyrhizobium sp. under continental conditions in Central Europe. Agriculture, 10: 446. https://doi.org/10.3390/agriculture10100446
 
Herridge D.F., Peoples M.B., Boddey R.M. (2008): Global inputs of biological nitrogen fixation in agricultural systems. Plant and Soil, 311: 1–18. https://doi.org/10.1007/s11104-008-9668-3
 
Kumar S., Stecher G., Tamura K. (2016): MEGA7: Molecular evolutionary genetic analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33: 1870–1874. https://doi.org/10.1093/molbev/msw054
 
Laguerre G., Allard M.-R., Revoy F., Amarger N. (1994): Rapid identification of rhizobia by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes. Applied and Environmental Microbiology, 60: 56–63.  https://doi.org/10.1128/aem.60.1.56-63.1994
 
Lindström K., Mousavi S.A. (2020): Effectiveness of nitrogen fixation in rhizobia. Microbial Biotechnology, 13: 1314–1335. https://doi.org/10.1111/1751-7915.13517
 
Raposeiras R., Marriel I.E., Muzzi M.R.S., Paiva E., Filho I.A.P., Carvalhais L.C., Passos R.V.M., Pinto P.P., de Sá N.M.H. (2006): Rhizobium strains competitiveness on bean nodulation in Cerrado soils. Pesquisa Agropecuária Brasileira, 41: 439–447. https://doi.org/10.1590/S0100-204X2006000300010
 
Šimon T., Salava J. (2006): New Rhizobium leguminosarum bv. trifolii isolates: evaluation of competitiveness for clover nodule occupancy. Plant, Soil and Environment, 52: 441–448. https://doi.org/10.17221/3464-PSE
 
Versalovic J., Koeuth T., Lupski J.R. (1991): Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Research, 19: 6823–6831. https://doi.org/10.1093/nar/19.24.6823
 
Vincent J.M. (1970): A Manual for the Practical Study of the Root-Nodule Bacteria. International Biological Programme Handbook. Oxford, Blackwell Scientific Publications.
 
Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. (1991): 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173: 697–703. https://doi.org/10.1128/jb.173.2.697-703.1991
 
Yates R.J., Howieson J.G., Reeve W.G., O’Hara G.W. (2011): A re-appraisal of the biology and terminology describing rhizobial strain success in nodule occupancy of legumes in agriculture. Plant and Soil, 348: 255–267. https://doi.org/10.1007/s11104-011-0971-z
 
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti