Ameliorating effects of exogenous paclobutrazol and putrescine on mung bean [Vigna radiata (L.) Wilczek] under water deficit stress

Babarashi E., Rokhzadi A., Pasari B., Mohammadi K. (2021): Ameliorating effects of exogenous paclobutrazol and putrescine on mung bean [Vigna radiata (L.) Wilczek] under water deficit stress. Plant Soil Environ., 67: 40–45.


download PDF

Plant growth regulators play crucial roles in modulating plant response to environmental stresses. In this experiment, the effect of different doses of paclobutrazol (PBZ) and putrescine (Put), i.e., 0, 50, 100 and 150 mg/L on mung bean in two conditions of water deficit (WD) and well-watered (WW) was investigated. The seed yield decreased due to water deficit stress, while the PBZ and Put application alleviated the damage of drought stress through increasing proline and leaf chlorophyll content and improving membrane stability, and thus increased plant yield compared to untreated control plants. According to regression equations, the high PBZ levels (150 mg/L or more) and moderate levels of Put (about 90 mg/L) were determined as the optimal concentrations to maximise mung bean yield in WD conditions. In WW conditions, the mung bean responses to PBZ were inconsistent, whereas Put application positively affected some physiological traits and seed yield. In conclusion, the physiological attributes and, subsequently, the seed yield of drought-stressed mung bean plants could be improved by foliar application of PBZ and Put.


Ashraf M., Foolad M.R. (2007): Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59: 206–216.
Bangar P., Chaudhury A., Tiwari B., Kumar S., Kumari R., Bhat K.V. (2019): Morphophysiological and biochemical response of mungbean [Vigna radiata (L.) Wilczek] varieties at different developmental stages under drought stress. Turkish Journal of Biology, 43: 58–69.
Bates L.S., Waldren R.P., Teare I.D. (1973): Rapid determination of free proline for water-stress studies. Plant and Soil, 39: 205–207.
Berova M., Zlatev Z. (2000): Physiological response and yield of paclobutrazol treated tomato plants (Lycopersicon esculentum Mill.). Plant Growth Regulation, 30: 117–123.
Besford R.T., Richardson C.M., Campos J.L., Tiburcio A.F. (1993): Effect of polyamines on stabilization of molecular complexes in thylakoid membranes of osmotically stressed oat leaves. Planta, 189: 201–206.
Bodner G., Nakhforoosh A., Kaul H.-P. (2015): Management of crop water under drought: a review. Agronomy for Sustainable Development, 35: 401–442.
Chand G., Nandwal A.S., Kumar N., Devi S., Khajuria S. (2018): Yield and physiological responses of mungbean Vigna radita (L.) Wilczek genotypes to high temperature at reproductive stage. Legume Research, 41: 557–562.
Chen D.D., Shao Q.S., Yin L.H., Younis A., Zheng B.S. (2019): Polyamine function in plants: metabolism, regulation on development, and roles in abiotic stress responses. Frontiers in Plant Science, 9: 1945.
Cohen I., Netzer Y., Sthein I., Gilichinsky M., Tel-Or E. (2019): Plant growth regulators improve drought tolerance, reduce growth and evapotranspiration in deficit irrigated Zoysia japonica under field conditions. Plant Growth Regulation, 88: 9–17.
Ebeed H.T., Hassan N.M., Aljarani A.M. (2017): Exogenous applications of Polyamines modulate drought responses in wheat through osmolytes accumulation, increasing free polyamine levels and regulation of polyamine biosynthetic genes. Plant Physiology and Biochemistry, 118: 438–448.
Kumar S., Ghatty S., Satyanarayana J., Guha A., Chaitanya B.S.K., Reddy A.R. (2012): Paclobutrazol treatment as a potential strategy for higher seed and oil yield in field-grown Camelina sativa L. Crantz. BMC Research Notes, 5: 137.
Lichthenthaler H.K. (1987): Chlorophylls and carotenoids – pigments of photosynthetic biomembranes. Methods in Enzymology, 148: 350–382.
Lutts S., Kinet J.M., Bouharmont J. (1996): NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany, 78: 389–398.
Mahdavian M., Sarikhani H., Hadadinejad M., Dehestani A. (2020): Putrescine effect on physiological, morphological, and biochemical traits of carrizo citrange and volkameriana rootstocks under flooding stress. International Journal of Fruit Science, 20: 164–177.
Nahar K., Hasanuzzaman M., Rahman A., Alam Md.M., Mahmud J.-A., Suzuki T., Fujita M. (2016): Polyamines confer salt tolerance in mung bean (Vigna radiata L.) by reducing sodium uptake, improving nutrient homeostasis, antioxidant defense, and methylglyoxal detoxification systems. Frontiers in Plant Science, 7: 1104.
Plaza-Wüthrich S., Blösch R., Rindisbacher A., Cannarozzi G., Tadele Z. (2016): Gibberellin deficiency confers both lodging and drought tolerance in small cereals. Frontiers in Plant Science, 7: 643.
Raina S.K., Govindasamy V., Kumar M., Singh A.K., Rane J., Minhas P.S. (2016): Genetic variation in physiological responses of mungbeans (Vigna radiata (L.) Wilczek) to drought. Acta Physiologiae Plantarum, 38: 263.
Senoo S., Isoda A. (2003): Effects of paclobutrazol on dry matter distribution and yield in peanut. Plant Production Science, 6: 90–94.
Singh P., Basu S., Kumar G. (2018): Polyamines metabolism: a way ahead for abiotic stress tolerance in crop plants. In: Wani S.H. (ed.): Biochemical, Physiological and Molecular Avenues for Combating Abiotic Stress Tolerance in Plants. San Diego, Academic Press, 39–55. ISBN: 978-0-12-813066-7
Soumya P.R., Kumar P., Pal M. (2017): Paclobutrazol: a novel plant growth regulator and multi-stress ameliorant. Indian Journal of Plant Physiology, 22: 267–278.
Tesfahun W. (2018): A review on: response of crops to paclobutrazol application. Cogent Food and Agriculture, 4: 1525169.
Yooyongwech S., Samphumphuang T., Tisarum R., Theerawitaya C., Cha-Um S. (2017): Water-deficit tolerance in sweet potato [Ipomoea batatas (L.) Lam.] by foliar application of paclobutrazol: role of soluble sugar and free proline. Frontiers in Plant Science, 8: 1400.
Zhu Y.S., Sun S., FitzGerald R. (2018): Mung bean proteins and peptides: nutritional, functional and bioactive properties. Food and Nutrition Research, 62: 1290.
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti