Response of Avena sativa, microorganisms and enzymes to contamination of soil with diesel oil J., Borowik A., Kucharski J. (2015): Response of Avena sativa, microorganisms and enzymes to contamination of soil with diesel oil. Plant Soil Environ., 61: 483-488.
download PDF
The scale of the impact of petroleum products on the natural environment is still difficult to determine. This is why it was decided to conduct tests, under the conditions of a pot experiment, for the effects of diesel oil (0, 4, and 8 mL/kg dry matter of soil) on the yield of oat, content of macroelements, as well as the microbiological, biochemical, physicochemical and chemical properties of the soil. The study results showed that diesel oil had an adverse effect on the growth and development of oat, and contents of nitrogen, sodium, calcium and magnesium in the above-ground parts of oat. Diesel oil increased the concentration of the following substances in the soil: naphthalene, phenanthrene, anthracene, benz[a]anthracene, chrysene, benzo[a]fluoranthene, benzo[a]pyrene and benzo[ghi]perylene, organic carbon, total nitrogen, and available potassium, while it decreased the concentration of available phosphorus and magnesium in the soil. Changes in the physicochemical properties of the soil had an unfavourable effect on the microbiological and biochemical properties. As regards the 7 tested enzymes, the most sensitive to diesel oil was catalase, and the least sensitive ones were ß-glucosidase and dehydrogenases. As for
12 various tested microorganisms, bacteria of the Azotobacter genus exhibited the highest resistance, while copiotrophic bacteria the lowest.
Baran Stanisław, Bielińska Jolanta E, Oleszczuk Patryk (2004): Enzymatic activity in an airfield soil polluted with polycyclic aromatic hydrocarbons. Geoderma, 118, 221-232
Borowik A., Wyszkowska J., Kucharski M., Kucharski J. (2014): Resistance of dehydrogenases, catalase, urease and plants to soil contamination with zinc. Journal of Elementology, 19: 929–946.
Gracia-Lor Emma, Sancho Juan V., Serrano Roque, Hernández Félix (2012): Occurrence and removal of pharmaceuticals in wastewater treatment plants at the Spanish Mediterranean area of Valencia. Chemosphere, 87, 453-462
Griffiths Bryan S., Philippot Laurent (): Insights into the resistance and resilience of the soil microbial community. FEMS Microbiology Reviews, 37, 112-129
Kucharski J., Jastrzębska E. (2005): Effects of heating oil on the number of microorganisms and physico-chemical properties of soil. Polish Journal of Environmental Studies, 14: 195–204.
Kucharski J., Jastrzębska E. (2006): Effect of heating oil on the activity of soil enzymes and the yield of yellow lupine. Plant, Soil and Environment, 52: 220–226.
Lebrero Raquel, Estrada José M., Muñoz Raúl, Quijano Guillermo (2012): Toluene mass transfer characterization in a biotrickling filter. Biochemical Engineering Journal, 60, 44-49
Lipińska Aneta, Kucharski Jan, Wyszkowska Jadwiga (): The Effect of Polycyclic Aromatic Hydrocarbons on the Structure of Organotrophic Bacteria and Dehydrogenase Activity in Soil. Polycyclic Aromatic Compounds, 34, 35-53
Nganje T. N., Edet A. E., Ekwere S. J. (2007): Distribution of PAHs in Surface Soils from Petroleum Handling Facilities in Calabar. Environmental Monitoring and Assessment, 130, 27-34
Orwin K.H., Wardle D.A. (2004): New indices for quantifying the resistance and resilience of soil biota to exogenous disturbances. Soil Biology and Biochemistry, 36, 1907-1912
Pezeshki S.R., Hester M.W., Lin Q., Nyman J.A. (2000): The effects of oil spill and clean-up on dominant US Gulf coast marsh macrophytes: a review. Environmental Pollution, 108, 129-139
Semrany Samer, Favier Lidia, Djelal Hayet, Taha Samir, Amrane Abdeltif (2012): Bioaugmentation: Possible solution in the treatment of Bio-Refractory Organic Compounds (Bio-ROCs). Biochemical Engineering Journal, 69, 75-86
Shrestha Reena Amatya, Pham Thuy Duong, Sillanpää Mika (2010): Electro ultrasonic remediation of polycyclic aromatic hydrocarbons from contaminated soil. Journal of Applied Electrochemistry, 40, 1407-1413
Sivitskaya V., Wyszkowski M. (2013): Changes in the content of some macroelements in maize (Zea mays L.) after application of fuel oil and different neutralizing substances to soil. Journal of Elementology, 18: 705–714.
Statsoft, Inc., Statistica (2014): Data Analysis Software System. Version 12.0. Available at
Vázquez S., Nogales B., Ruberto L., Mestre C., Christie-Oleza J., Ferrero M., Bosch R., Mac Cormack W.P. (2013): Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation. International Biodeterioration & Biodegradation, 77, 22-30
Wu B., Lan T., Lu D., Liu Z. (2014): Ecological and enzymatic responses to petroleum contamination. Environmental Science: Processes and Impacts, 16: 1501–1509.
Wyszkowska J., Borowik A., Kucharski J., Baćmaga M., Tomkiel M., Boros-Lajszner E. (2013): The effect of organic fertilizers on the biochemical properties of soil contaminated with zinc. Plant, Soil and Environment, 59: 500–504.
Wyszkowska J., Kucharski J. (2005): Correlation between the number of cultivable microorganisms and soil contamination with diesel oil. Polish Journal of Environmental Studies, 14: 347–356.
Wyszkowska J., Kucharski M., Kucharski J. (2006): Application of the activity of soil enzymes in the evaluation of soil contamination by diesel oil. Polish Journal of Environmental Studies, 15: 501–506.
Wyszkowska J., Boros E., Kucharski J. (2007): Effect of interactions between nickel and other heavy metals on the soil microbiological properties. Plant, Soil and Environment, 53: 544–552.
Wyszkowski M., Ziółkowska A. (2013): Content of polycyclic aromatic hydrocarbons in soils polluted with petrol and diesel oil after remediation with plants and various substances. Plant, Soil and Environment, 59: 287–294.
Zhan Xinhua, Wu Wenzhu, Zhou Lixiang, Liang Jianru, Jiang Tinghui (2010): Interactive effect of dissolved organic matter and phenanthrene on soil enzymatic activities. Journal of Environmental Sciences, 22, 607-614
download PDF

© 2019 Czech Academy of Agricultural Sciences