Salt acclimation induced salt tolerance in wild-type and abscisic acid-deficient mutant barley Z., Guo J., Xin C., Liu S., Mao H., Wang Y., Li X. (2019): Salt acclimation induced salt tolerance in wild-type and abscisic acid-deficient mutant barley. Plant Soil Environ., 65: 516-521.
download PDF

Salt acclimation is a process to enhance salt tolerance in plants. The salt acclimation induced salt tolerance was investigated in a spring barley (Hordeum vulgare L.) cv. Steptoe (wild type, WT) and its abscisic acid (ABA)-deficient mutant Az34. Endogenesis ABA concentration in leaf was significantly increased by salt stress in WT, while it was not affected in Az34. Under salt stress, the salt acclimated Az34 plants had 14.8% lower total soluble sugar concentration and 93.7% higher sodium (Na) concentration in leaf, compared with salt acclimated WT plants. The acclimated plants had significantly higher leaf water potential and osmotic potential than non-acclimated plants in both WT and Az34 under salt stress. The salt acclimation enhanced the net photosynthetic rate (by 22.9% and 12.3%) and the maximum quantum yield of PS II (22.7% and 22.0%) in WT and Az34 under salt stress. However, the stomatal conductance in salt acclimated Az34 plants was 28.9% lower than WT under salt stress. Besides, the guard cell pair width was significantly higher in salt acclimated Az34 plants than that in WT plants. The results indicated that the salt acclimated WT plants showed a higher salt tolerance than Az34 plants, suggesting that ABA deficiency has a negative effect on the salt acclimation induced salt tolerance in barley.

Dodd I.C., Davies W.J. (1994): Leaf growth responses to ABA are temperature dependent. Journal of Experimental Botany, 45: 903–907.
El-Esawi M.A., Alaraidh I.A., Alsahli A.A., Ali H.M., Alayafi A.A., Witczak J., Ahmad M. (2018): Genetic variation and alleviation of salinity stress in barley (Hordeum vulgare L.). Molecules, 23: 2488–2504.
Garcia de la Garma J., Fernandez-Garcia N., Bardisi E., Pallol B., Asensio-Rubio J.S., Bru R., Olmos E. (2015): New insights into plant salt acclimation: The roles of vesicle trafficking and reactive oxygen species signaling in mitochondria and the endomembrane system. New Phytologist, 205: 216–239.
Jakab G., Ton J., Flors V., Zimmerli L., Metraux J.-P., Mauch-Mani B. (2005): Enhancing Arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses. Plant Physiology, 139: 267–274.
Janda T., Darko É., Shehata S., Kovács V., Pál M., Szalai G. (2016): Salt acclimation processes in wheat. Plant Physiology and Biochemistry, 101: 68–75.
Karmoker J.L., Van Steveninck R.F.M. (1979): The effect of abscisic acid on the uptake and distribution of ions in intact seedlings of Phaseolus vulgaris cv. Redland Pioneer. Physiological Plantarum, 45: 453–459.
Li X.N., Jiang H.D., Liu F.L., Cai J., Dai T.B., Cao W.X., Jiang D. (2013): Induction of chilling tolerance in wheat during germination by pre-soaking seed with nitric oxide and gibberellin. Plant Growth Regulation, 71: 31–40.
Li X.N., Topbjerg H.B., Jiang D., Liu F.L. (2015): Drought priming at vegetative stage improves the antioxidant capacity and photosynthesis performance of wheat exposed to short-term low-temperature stress at the jointing stage. Plant and Soil, 393: 307–318.
Liang W.J., Ma X.L., Wan P., Liu L.Y. (2018): Plant salt-tolerance mechanism: A review. Biochemical and Biophysical Research Communications, 495: 286–291.
Mahlooji M., Seyed Sharifi R., Razmjoo J., Sabzalian M.R., Sedghi M. (2017): Effect of salt stress on photosynthesis and physiological parameters of three contrasting barley genotypes. Photosynthetica, 56: 549–556.
Merilo E., Yarmolinsky D., Jalakas P., Parik H., Tulva I., Rasulov B., Kilk K., Kollist H. (2018): Stomatal VPD response: There is more to the story than ABA. Plant Physiology, 176: 851–864.
Munns R., Tester M. (2008): Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59: 651–681.
Pandolfi C., Azzarello E., Mancuso S., Shabala S. (2016): Acclimation improves salt stress tolerance in Zea mays plants. Journal of Plant Physiology, 201: 1–8.
Parida A.K., Das A.B. (2005): Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety, 60: 324–349.
Sun Z.W., Ren L.K., Fan J.W., Li Q., Wang K.J., Guo M.M., Wang L., Li J., Zhang G.X., Yang Z.Y., Chen F., Li X.N. (2016): Salt response of photosynthetic electron transport system in wheat cultivars with contrasting tolerance. Plant, Soil and Environment, 62: 515–521.
Tabassum T., Ahmad R., Farooq M., Basra S.M.A. (2018): Improving salt tolerance in barley by osmopriming and biopriming. International Journal of Agriculture and Biology, 20: 2455–2464.
Tang X.L., Mu X.M., Shao H.B., Wang H.Y., Brestič M. (2015): Global plant-responding mechanisms to salt stress: Physiological and molecular levels and implications in biotechnology. Critical Reviews in Biotechnology, 35: 425–437.
Tavakkoli E., Fatehi F., Coventry S., Rengasamy P., McDonald G.K. (2011): Additive effects of Na+ and Cl– ions on barley growth under salinity stress. Journal of Experimental Botany, 62: 2189–2203.
Wang Z.S., Li X.N., Zhu X.C., Liu S.Q., Song F.B., Liu F.L., Wang Y., Qi X.N., Wang F.H., Zuo Z.Y., Duan P.Z., Yang A.Z., Cai J., Jiang D. (2017): Salt acclimation induced salt tolerance is enhanced by abscisic acid priming in wheat. Plant, Soil and Environment, 63: 307–314.
Wu D.Z., Shen Q.F., Qiu L., Han Y., Ye L.Z., Jabeen Z., Shu Q.Y., Zhang G.P. (2014): Identification of proteins associated with ion homeostasis and salt tolerance in barley. Proteomics, 14: 1381–1392.
Živčák M., Brückova K., Sytar O., Brestič M., Olšovská K., Allakhverdiev S.I. (2017): Lettuce flavonoids screening and phenotyping by chlorophyll fluorescence excitation ratio. Planta, 245: 1215–1229.
Zuo Z.Y., Li X.N., Xu C., Yang J.J., Zhu X.C., Liu S.Q., Song F.B., Liu F.L., Mao H.P. (2017): Responses of barley Albina and Xantha mutants deficient in magnesium chelatase to soil salinity. Plant, Soil and Environment, 63: 348–354.
download PDF

© 2019 Czech Academy of Agricultural Sciences