Indole-3-acetic acid synthesizing chromium-resistant bacteria can mitigate chromium toxicity in Helianthus annuus L.

https://doi.org/10.17221/581/2019-PSECitation:Ahmed A., Fatima H. (2020): Indole-3-acetic acid synthesizing chromium-resistant bacteria can mitigate chromium toxicity in Helianthus annuus L. Plant Soil Environ., 66: 216-221.
download PDF

Use of microorganisms as heavy metal remediators is an effective approach for chromium reduction in plants. Chromium carcinogenicity (Cr6+) beyond the permissible levels elicits environmental and health problems. To reduce chromium toxicity along with the plant growth improvement, a cost-effective and eco-friendly remediation approach is necessary. In the current study, chromium-resistant bacterial species were evaluated for growth improvement of sunflower. Three auxin-producing bacteria able to tolerate hexavalent chromium, i.e., Sporosarcina saromensis (EI) and two species of Bacillus cereus (AR and 3a) were selected for the proposed study. Growth studies along with auxin synthesis potential of bacterial isolates with and without chromium were conducted. Results revealed a 188% enhancement in plant height under laboratory-grown plants with B. cereus (AR) under 500 mg/L chromium stress (Cr6+). B. cereus (3a) also showed an 81% increase in leaf number with 400 mg/L chromium stress in laboratory-grown plants. Similarly, 73% increment in the amount of auxin was reported in the case of inoculation with S. saromensis isolate (EI) over respective control treatment. These improvements provide an excellent means of reducing chromium (Cr6+) in the contaminated soils naturally by stimulating plant growth along with bioremediation potential.

 

References:
Ali J., Mahmood T., Hayat K., Afridi M.S., Ali F., Chaudhary H.J. (2018): Phytoextraction of Cr by maize (Zea mays L.): the role of plant growth promoting endophyte and citric acid under polluted soil. Archives of Environmental Protection, 44: 73–82.
 
Bates L.S., Waldren R.P., Teare I.D. (1973): Rapid determination of free proline for water-stress studies. Plant and Soil, 39: 205–207. https://doi.org/10.1007/BF00018060
 
Cappuccino J.G., Sherman N. (2007): Microbiology: A Laboratory Manual. 7th Edition. San Francisco, Benjamin Cummings. ISBN-13: 978-0805328363
 
Chen Y.W., Wang L., Dai F.Z., Tao M., Li X.D., Tan Z.L. (2019): Biostimulants application for bacterial metabolic activity promotion and sodium dodecyl sulfate degradation under copper stress. Chemosphere, 226: 736–743. https://doi.org/10.1016/j.chemosphere.2019.03.180
 
Fatima H., Ahmed A. (2016): How chromium-resistant bacteria can improve corn growth in chromium-contaminated growing medium. Polish Journal of Environmental Studies, 25: 2357–2365. https://doi.org/10.15244/pjoes/63334
 
Ferjani R., Gharsa H., Estepa-Pérez V., Gómez-Sanz E., Cherni M., Mahjoubi M., Boudabous A., Torres C., Ouzari H.-I. (2019): Plant growth-promoting Rhizopseudomonas: expanded biotechnological purposes and antimicrobial resistance concern. Annals of Microbiology, 69: 51–59. https://doi.org/10.1007/s13213-018-1389-0
 
Francisco R., Branco R., Schwab S., Baldani J.I., Morais P.V. (2017): Impact of plant-associated bacteria biosensors on plant growth in the presence of hexavalent chromium. World Journal of Microbiology and Biotechnology, 34: 12. https://doi.org/10.1007/s11274-017-2389-0
 
Gupta P., Rani R., Chandra A., Varjani S.J., Kumar V. (2017): Effectiveness of plant growth-promoting rhizobacteria in phytoremediation of chromium stressed soils. In: Varjani S.J., Gnansounou E., Gurunathan B., Pant D., Zakaria Z.A. (eds.): Waste Bioremediation. Singapore, Springer, 301–312. ISBN: 978-981-10-7412-7
 
Habib S., Fatima H., Ahmed A. (2019): Comparative analysis of pre-germination and post-germination inoculation treatments of Zea mays L. to mitigate chromium toxicity in Cr-contaminated soils. Polish Journal of Environmental Studies, 28: 597–607. https://doi.org/10.15244/pjoes/81570
 
Ju W.L., Liu L., Fang L., Cui Y., Duan C., Wu H. (2019): Impact of co-inoculation with plant-growth-promoting rhizobacteria and rhizobium on the biochemical responses of alfalfa-soil system in copper contaminated soil. Ecotoxicology and Environmental Safety, 167: 218–226. https://doi.org/10.1016/j.ecoenv.2018.10.016
 
Khanna K., Jamwal V.L., Kohli S.K., Gandhi S.G., Ohri P., Bhardwaj R., Abd_Allah E.F., Hashem A., Ahmad P. (2019): Plant growth promoting rhizobacteria induced Cd tolerance in Lycopersicon esculentum through altered antioxidative defense expression. Chemosphere, 217: 463–474. https://doi.org/10.1016/j.chemosphere.2018.11.005
 
Levizou E., Zanni A.A., Antoniadis V. (2019): Varying concentrations of soil chromium (VI) for the exploration of tolerance thresholds and phytoremediation potential of the oregano (Origa- https://doi.org/10.1007/s11356-018-2658-y
 
num vulgare). Environmental Science and Pollution Research, 26: 14–23.
 
Lichtenthaler H.K., Wellburn A.R. (1983): Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11: 591–592. https://doi.org/10.1042/bst0110591
 
Lowry O.H., Resebrough N.J., Farr A.L., Randall R.J. (1995): Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry, 193: 265–275.
 
Mahadevan A. (1984): Auxin in microorganisms and infected plants. In: Mahadevan A. (ed.): Growth Regulators, Microorganisms and Diseased Plants. India, Oxford and IBH Publishing Company, 31: 265–343.
 
Motsara M.R., Roy R.N. (2008): Soil analysis. In: Motsara M.R., Roy R.N. (eds.): Guide to Laboratory Establishment for Plant Nutrient Analysis. Fao Fertilizer and Plant Nutrition Bulletin 19. Rome, Food and Agriculture Organisation of the United Nations, 51–66
 
Nafees M., Ali S., Naveed M., Rizwan M. (2018): Efficiency of biogas slurry and Burkholderia phytofirmans PsJN to improve growth, physiology, and antioxidant activity of Brassica napus L. https://doi.org/10.1007/s11356-017-0924-z
 
in chromium-contaminated soil. Environmental Science and Pollution Research, 25: 6387–6397.
 
Oves M., Khan M.S., Qari H.A. (2019): Chromium-reducing and phosphate-solubilizing Achromobacter xylosoxidans bacteria from the heavy metal-contaminated soil of the Brass city, Moradabad, India. International Journal of Environmental Science and Technology, 16: 6967–6984. https://doi.org/10.1007/s13762-019-02300-y
 
Ripa F.A., Cao W.D., Tong S., Sun J.G. (2019): Assessment of plant growth promoting and abiotic stress tolerance properties of wheat endophytic fungi. BioMed Research International, 2019: 6105865. https://doi.org/10.1155/2019/6105865
 
Rizvi A., Ahmed B., Zaidi A., Khan M.S. (2019): Bioreduction of toxicity influenced by bioactive molecules secreted under metal stress by Azotobacter chroococcum. Ecotoxicology, 28: 302–322. https://doi.org/10.1007/s10646-019-02023-3
 
Rocha S.M.B., Antunes J.E.L., Araujo J.M.A., Aquino J.P.A., Melo W.J., Mendes L.W., Araujo A.S.F. (2019): Capability of plant growth-promoting bacteria in chromium-contaminated soil after application of composted tannery sludge. Annals of Microbiology, 21: 1–7. https://doi.org/10.1007/s13213-019-01455-w
 
Solá M.Z.S., Lovaisa N., Costa J.S.D., Benimeli C.S., Polti M.A., Alvarez A. (2019): Multi-resistant plant growth-promoting actinobacteria and plant root exudates influence Cr(VI) and lindane dissipation. Chemosphere, 222: 679–687. https://doi.org/10.1016/j.chemosphere.2019.01.197
 
Stambulska U.V., Bayliak M.M., Lushchak V.L. (2018): Chromium (VI) toxicity in legume plants: modulation effects of rhizobial symbiosis. BioMed Research International, 2018: 1–13.
 
Yahaghi Z., Shirvani M., Nourbakhsh F., Pueyo J.J. (2019): Uptake and effects of lead and zinc on alfalfa (Medicago sativa L.) seed germination and seedling growth: role of plant growth promoting https://doi.org/10.1016/j.sajb.2019.01.006
 
bacteria. South African Journal of Botany, 124: 573–582.
 
Zhou W.J., Long W.J., Xu T., Peng L.Q., Zhang W.H. (2019): Organic ligands unexpectedly increase the toxicity of chromium (III) for luminescent bacteria. Environmental Chemistry Letters, 17: 1849–1855. https://doi.org/10.1007/s10311-019-00892-y
 
Zunji S., Shen W., Yang K., Zheng N., Jiang X., Liu L., Yang D., Zhang L., Ai Z., Xie B. (2019): Hexavalent chromium removal by a new composite system of dissimilatory iron reduction bacteria Aeromonas hydrophila and nanoscale zero-valent iron. Chemical Engineering Journal, 362: 63–70. https://doi.org/10.1016/j.cej.2019.01.030
 
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti