Optimisation of diallyl disulfide concentration and effect of soil condition on urease inhibition
Diallyl disulfide (DADS) is an organosulfur compound that is expected to exhibit inhibitory property against urease similar to allicin, affirmed through preliminary study. The research aims to optimize DADS’s concentration and duration of inhibition and observe the effect of soil moisture, temperature and pH on the inhibitory action of DADS. The calorimetric method was applied to optimize DADS’s concentration significant for inhibition. High-performance liquid chromatography was used to quantify DADS present under different parameters relevant to selected soil conditions. The results obtained suggested that 5% of DADS/urea-N (w/w) treatment exhibited the highest urea hydrolysis reduction by 27.91% compared to the control sample at the end of 30 days. ANOVA results observed urea hydrolysis is significantly slower by applying 5% DADS/urea-N (w/w) treatment compared to the other DADS treatments. DADS also retained its original form longer in soil when the soil conditions were altered to 15% moisture content, 20 °C and pH 4. The findings exhibit the potential of DADS as a natural based inhibitor that is effective at low concentrations, compatible with urea and chemically stable.
Artola E., Cruchaga S., Ariz I., Moran J.F., Garnica M., Houdusse F., Mina J.M.G., Irigoyen I., Lasa B., Aparicio-Tejo P.M. (2011): Effect of N-(n-butyl) thiophosphoric triamide on urea metabolism and the assimilation of ammonium by Triticum aestivum L. Plant Growth Regulation, 63: 73–79. https://doi.org/10.1007/s10725-010-9513-6
Cameron K.C., Di H.J., Moir J.L. (2013): Nitrogen losses from the soil/plant system: a review. Annals of Applied Biology, 162: 145–173. https://doi.org/10.1111/aab.12014
Dawar K., Zaman M., Rowarth J.S., Turnbull M.H. (2012): Applying urea with urease inhibitor (N-(n-butyl) thiophosphoric triamide) in fine particle application improves nitrogen uptake in ryegrass (Lolium perenne L.). Soil Science and Plant Nutrition, 58: 309–318. https://doi.org/10.1080/00380768.2012.680050
George J., Holtham L., Sabermanesh K., Heuer S., Tester M.A., Plett D., Garnett T. (2016): Small amounts of ammonium (NH4+) can increase growth of maize (Zea mays). Journal of Plant Nutrition and Soil Science, 179: 717–725. https://doi.org/10.1002/jpln.201500625
Gerbens-Leenes P.W., Nonhebel S., Ivens W.P.M.F. (2002): A method to determine land requirements relating to food consumption patterns. Agriculture, Ecosystems and Environment, 90: 47–58. https://doi.org/10.1016/S0167-8809(01)00169-4
Halvorson A.D., Snyder C.S., Blaylock A.D., Del Grosso S.J. (2014): Enhanced-efficiency nitrogen fertilizers: potential role in nitrous oxide emission mitigation. Agronomy Journal, 106: 715–722. https://doi.org/10.2134/agronj2013.0081
Iberl B., Winkler G., Knobloch K. (1990): Products of allicin transformation: ajoenes and dithiins, characterization and their determination by HPLC. Planta Medica, 56: 202–211. https://doi.org/10.1055/s-2006-960926
Juszkiewicz A., Zaborska A., Łaptaś A., Olech Z. (2004): A study of the inhibition of jack bean urease by garlic extract. Food Chemistry, 85: 553–558. https://doi.org/10.1016/j.foodchem.2003.07.033
Krajewska B. (2009): Ureases I. Functional, catalytic and kinetic properties: a review. Journal of Molecular Catalysis B: Enzymatic, 59: 9–21. https://doi.org/10.1016/j.molcatb.2009.01.003
Lawson L.D., Wood S.G., Hughes B.G. (1991): HPLC analysis of allicin and other thiosulfinates in garlic clove homogenates. Planta Medica, 57: 263–270. https://doi.org/10.1055/s-2006-960087
Mathialagan R., Mansor N., Al-Khateeb B., Mohamad M.H., Shamsuddin M.R. (2017): Evaluation of allicin as soil urease inhibitor. Procedia Engineering, 184: 449–459. https://doi.org/10.1016/j.proeng.2017.04.116
Modolo L.V., de Souza A.X., Horta L.P., Araujo D.P., de Fátima Â. (2015): An overview on the potential of natural products as ureases inhibitors: a review. Journal of Advanced Research, 6: 35–44. https://doi.org/10.1016/j.jare.2014.09.001
Mohanty S., Patra A.K., Chhonkar P.K. (2008): Neem (Azadirachta indica) seed kernel powder retards urease and nitrification activities in different soils at contrasting moisture and temperature regimes. Bioresource Technology, 99: 894–899. https://doi.org/10.1016/j.biortech.2007.01.006
Moyo C.C., Kissel D.E., Cabrera M.L. (1989): Temperature effects on soil urease activity. Soil Biology and Biochemistry, 21: 935–938. https://doi.org/10.1016/0038-0717(89)90083-7
Mulvaney R.L., Bremner J.M. (1979): A modified diacetyl monoxime method for colorimetric determination of urea in soil extracts. Communications in Soil Science and Plant Analysis, 10: 1163–1170. https://doi.org/10.1080/00103627909366969
Naganawa R., Iwata N., Ishikawa K., Fukuda H., Fujino T., Suzuki A. (1996): Inhibition of microbial growth by ajoene, a sulfur-containing compound derived from garlic. Applied and Environmental Microbiology, 62: 4238–4242. https://doi.org/10.1128/AEM.62.11.4238-4242.1996
Nair S., Mukne A. (2017): Assessment of chemical stability of constituents in thiosulfinate derivative-rich extract of garlic by a Validated HPTLC method. Indian Journal of Pharmaceutical Sciences, 79: 438–450. https://doi.org/10.4172/pharmaceutical-sciences.1000247
Olech Z., Zaborska W., Kot M. (2014): Jack bean urease inhibition by crude juices of Allium and Brassica plants. Determination of thiosulfinates. Food Chemistry, 145: 154–160. https://doi.org/10.1016/j.foodchem.2013.08.044
Rabinkov A., Miron T., Konstantinovski L., Wilchek M., Mirelman D., Weiner L. (1998): The mode of action of allicin: trapping of radicals and interaction with thiol containing proteins. Biochimica et Biophysica Acta (BBA) – General Subjects, 1379: 233–244. https://doi.org/10.1016/S0304-4165(97)00104-9
Sahrawat K.L. (1984): Effects of temperature and moisture on urease activity in semi-arid tropical soils. Plant and Soil, 78: 401–408. https://doi.org/10.1007/BF02450373
Sekaran U., McCoy C., Kumar S., Subramanian S. (2019): Soil microbial community structure and enzymatic activity responses to nitrogen management and landscape positions in switchgrass (Panicum virgatum L.). GCB Bioenergy, 11: 836–851. https://doi.org/10.1111/gcbb.12591
Shaviv A., Mikkelsen R.L. (1993): Controlled-release fertilizers to increase efficiency of nutrient use and minimize environmental degradation – A review. Fertilizer Research, 35: 1–12. https://doi.org/10.1007/BF00750215
Sheo Bachan Upadhyay L. (2012): Urease inhibitors: a review. Indian Journal of Biotechnology, 11: 381–388.
Vahed H.S., Shahinrokhsar P., Rezaei M. (2011): Influence of some soil properties and temperature on urease activity in wetland rice soils. American-Eurasian Journal of Agricultural. Environmental Sciences, 11: 310–313.
Wan X., Polyakova Y., Row K. (2007): Determination of diallyl disulfide in garlic by reversed-phase high performance liquid chromatography. Analytical Science and Technology, 20.
Yu T.H., Wu C.M., Chen S.Y. (1989): Effects of pH adjustment and heat treatment on the stability and the formation of volatile compounds of garlic. Journal of Agricultural and Food Chemistry, 37: 730–734. https://doi.org/10.1021/jf00087a033