Characterisation of Bt maize IE09S034 in decomposition and response of soil bacterial communities

Zhou X.L., Liang J.G., Luan Y., Song X.Y., Zhang Z.G. (2021): Characterisation of Bt maize IE09S034 in decomposition and response of soil bacterial communities. Plant Soil Environ., 67: 286–298.


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Returning straw to the soil is an effective way to improve the soil quality. As genetically modified (GM) crops experience expanded growing scales, returning straw to the soil could also be necessary. However, the impact of GM crop straws on soil safety remains unclear. The environment (including soil types, humidity and temperature) can result in a significant difference in the diversity of soil bacterial communities. Here, we compared the impacts of the straw from Bt maize IE09S034 (IE) and near-isogenic non-Bt maize Zong31 (CK) on soil bacterial community and microbial metabolic activity in three different environments. Sampling was carried out following 6–10 months of decomposition (May, June, July, and August) in three localities in Chinese cities (Changchun, Jinan, and Beijing). Our results showed that Bt maize residues posed no direct impact on soil bacterial communities in contrast to the environment and decomposed time. The microbial functional diversity and metabolic activity showed no significant difference between IE and CK. The results could be a reference for further assessing the effect of Bt maize residues on the soil that promotes the commercialisation of Bt maize IE09S034.

Cao B., Zhang Y., Wang Z.Y., Li M.Y., Yang F., Jiang D., Jiang Z. (2018): Insight into the variation of bacterial structure in atrazine-contaminated soil regulating by potential phytoremediator: Pennisetum americanum (L.) K. Schum. Frontiers in Microbiology, 9: 864.
DeBruyn J.M., Nixon L.T., Fawaz M.N., Johnson A.M., Radosevich M. (2011): Global biogeography and quantitative seasonal dynamics of Gemmatimonadetes in soil. Applied Environmental Microbiology, 77: 6295–6300.
Dedysh S.N., Ivanova A.A. (2019): Planctomycetes in boreal and subarctic wetlands: diversity patterns and potential ecological functions. FEMS Microbiology Ecology, 95: fiy227.
Dennis P.G., Newsham K.K., Rushton S.P., O'Donnell A.G., Hopkins D.W. (2019): Soil bacterial diversity is positively associated with air temperature in the maritime Antarctic. Science Report, 9: 2686.
Dohrmann A.B., Küting M., Jünemann S., Jaenicke S., Schlüter A., Tebbe C.C. (2013): Importance of rare taxa for bacterial diversity in the rhizosphere of Bt- and conventional maize varieties. The ISME Journal, 7: 37–49.
Fan C.M., Wu F.C., Dong J.Y., Wang B.F., Yin J.Q., Song X.Y. (2019): No impact of transgenic cry1Ie maize on the diversity, abundance and composition of soil fauna in a 2-year field trial. Scientific Reports, 9: 10333.
Fang M., Motavalli P.P., Kremer R.J., Nelson K.A. (2007): Assessing changes in soil microbial communities and carbon mineralization in Bt and non-Bt corn residue-amended soils. Applied Soil Ecology, 37: 150–160.
Guo J.F., He K.L., Bai S.X., Zhang T.T., Liu Y.J., Wang F.X., Wang Z.Y. (2016): Effects of transgenic cry1Ie maize on non-lepidopteran pest abundance, diversity and community composition. Transgenic Research, 25: 761–772.
Han L.Z., Jiang X.F., Peng Y.F. (2016): Potential resistance management for the sustainable use of insect-resistant genetically modified corn and rice in China. Current Opinion in Insect Science, 15: 139–143.
Hong C., Si Y.X., Xing Y., Li Y. (2015): Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas. Environmental Science and Pollution Research, 22: 10788–10799.
ISAAA (2018): Global status of commercialized biotech/GM crops in 2018: Biotech crops continue to help meet the challenges of increased population and climate change. ISAAA Brief, No. 54.
Kamle M., Kumar P., Patra J.K., Bajpai V.K. (2017): Current perspectives on genetically modified crops and detection methods. 3 Biotech, 7: 219.
Kozich J.J., Westcott S.L., Baxter N.T., Highlander S.K., Schloss P.D. (2013): Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Applied and Environmental Microbiology, 79: 5112–5120.
Kumar S., Chandra A., Pandey K.C. (2008): Bacillus thuringiensis (Bt) transgenic crop: an environment friendly insect-pest management strategy. Journal of Environmental Biology, 29: 641–653.
Liang J.G., Luan Y., Jiao Y., Xin L.T., Song X.Y., Zheng X.B., Zhang Z.G. (2018): No significant differences in rhizosphere bacterial communities between Bt maize cultivar IE09S034 and the near-isogenic non-Bt cultivar Zong31. Plant, Soil and Environment, 64: 427–434.
Liang J.G., Xin L.T., Meng F., Sun S., Wu C.X., Wu H.Y., Zhang M.R., Zhang H.F., Zheng X.B., Zhang Z.G. (2016): High-methionine soybean has no adverse effect on functional diversity of rhizosphere microorganisms. Plant, Soil and Environment, 62: 441–446.
Losey J.E., Rayor L.S., Carter M.E. (1999): Transgenic pollen harms monarch larvae. Nature, 399: 214.
Lu G.H., Tang C.Y., Hua X.M., Cheng J., Wang G.H., Zhu Y.L., Zhang L.Y., Shou H.X., Qi J.L., Yang Y.H. (2018a): Effects of an EPSPS-transgenic soybean line ZUTS31 on root-associated bacterial communities during field growth. PloS One, 13: e0192008.
Lu G.H., Hua X.M., Liang L., Wen Z.L., Du M.H., Meng F.F., Pang Y.J., Qi J.L., Tang C.Y., Yang Y.H. (2018b): Identification of major rhizobacterial taxa affected by a glyphosate-tolerant soybean line via shotgun metagenomic approach. Genes (Basel), 9: 214.
Luo X., Fu X., Yang Y., Cai P., Peng S., Chen W., Huang Q. (2016): Microbial communities play important roles in modulating paddy soil fertility. Scientific Reports, 6: 20326.
Mulder C., Wouterse M., Raubuch M., Roelofs W., Rutgers M. (2006): Can transgenic maize affect soil microbial communities? PLoS Computational Biology, 2: e128.
Peiffer J.A., Spor A., Koren O., Jin Z., Tringe S.G., Dangl J.L., Buckler E.S., Ley R.E. (2013): Diversity and heritability of the maize rhizosphere microbiome under field conditions. Proceedings of the National Academy of Sciences of the United States of America, 110: 6548–6553.
R Development Core Team (2011): R: A language and Environment for Statistical Computing. Vienna, R Foundation for Statistical Computing.
Schloss P.D., Westcott S.L., Ryabin T., Hall J.R., Hartmann M., Hollister E.B., Lesniewski R.A., Oakley B.B., Parks D.H., Robinson C.J., Sahl J.W., Stres B., Thallinger G.G., Van Horn D.J., Weber C.F. (2009): Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied Environmental Microbiology, 75: 7537–7541.
Shu Y.H., Zhang Y.Y., Zeng H.L., Zhang Y.H., Wang J.W. (2017): Effects of Cry1Ab Bt maize straw return on bacterial community of earthworm Eisenia fetida. Chemosphere, 173: 1–13.
Song F.P., Zhang J., Gu A.X., Wu Y., Han L.L., He K.L., Chen Z.Y., Yao J., Hu Y.Q., Li G.X., Huang D.F. (2003): Identification of cry1I-type genes from Bacillus thuringiensis strains and characterization of a novel cry1I-type gene. Applied and Environmental Microbiology, 69: 5207–5211.
Sohn S.I., Oh Y.J., Kim B.Y., Cho H.S. (2016): Effects of CaMSRB2-expressing transgenic rice cultivation on soil microbial communities. Journal of Microbiology and Biotechnology, 26: 1303–1310.
Su Y., Yu M., Xi H., Lv J.L., Ma Z.H., Kou C.L., Shen A. (2020a): Soil microbial community shifts with long-term of different straw return in wheat-corn rotation system. Scientific Reports, 10: 6360.
Su Y., Lv J.L., Yu M., Ma Z.H., Xi H., Kou C.L., He Z.C., Shen A.L. (2020b): Long-term decomposed straw return positively affects the soil microbial community. Journal of Applied Microbiology, 128: 138–150.
Szoboszlay M., Näther A., Mullins E., Tebbe C.G. (2019): Annual replication is essential in evaluating the response of the soil microbiome to the genetic modification of maize in different biogeographical regions. PLoS One, 14: e0222737.
Takahashi S., Uenosono S., Ono S. (2003): Short- and long-term effects of rice straw application on nitrogen uptake by crops and nitrogen mineralization under flooded and upland conditions. Plant and Soil, 251: 291–301.
Thottathil S.D., Balachandran K.K., Jayalakshmy K.V., Gupta G.V.M., Nair S. (2008): Tidal switch on metabolic activity: salinity induced responses on bacterioplankton metabolic capabilities in a tropical estuary. Estuarine, Coastal and Shelf Science, 78: 665–673.
Van Wyk D.A.B., Adeleke R., Rhode O.H.J., Bezuidenhout C.C., Mienie C. (2017): Ecological guild and enzyme activities of rhizosphere soil microbial communities associated with Bt-maize cultivation under field conditions in North West Province of South Africa. Journal of Basic Microbiology, 57: 781–792.
Wen Z.L., Yang M.K., Du M.H., Zhong Z.Z., Lu Y.T., Wang G.H., Hua X.M., Fazal A., Mu C.H., Yan S.F., Zhen Y., Yang R.W., Qi J.L., Hong Z., Lu G.H., Yang Y.H. (2019): Enrichments/derichments of root-associated bacteria related to plant growth and nutrition caused by the growth of an EPSPS-transgenic maize line in the field. Frontiers Microbiology, 10: 01335.
Xue K., Serohijos R.C., Devare M., Thies J.E. (2011): Decomposition rates and residue-colonizing microbial communities of Bacillus thuringiensis insecticidal protein Cry3Bb-expressing (Bt) and non-Bt corn hybrids in the field. Applied and Environmental Microbiology, 77: 839–846.
Zhang Y.T., Shen H., He X.H., Thomas B.W., Lupwayi N.Z., Hao X.Y., Thomas M.C., Shi X.J. (2017): Fertilization shapes bacterial community structure by alteration of soil pH. Frontiers Microbiology, 8: 1325.
Zhou D.G., Xu L.P., Gao S.W., Guo J.L., Luo J., You Q., Que Y.X. (2016): Cry1Ac transgenic sugarcane does not affect the diversity of microbial communities and has no significant effect on enzyme activities in rhizosphere soil within one crop season. Frontiers in Plant Science, 7: 265.
Zwahlen C., Hilbeck A., Gugerli P., Nentwig W. (2003): Degradation of the Cry1Ab protein within transgenic Bacillus thuringiensis corn tissue in the field. Molecular Ecology, 12: 765–775.
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