Effects of mowing dominant grasses on root exudation and soil nitrogen cycling in a natural sod culture apple orchard


Zhou E.D., Gou M.C., Yu B., Sun C., He J.L., Qin S.J., Lyu D.G. (2021): Effects of mowing dominant grasses on root exudation and soil nitrogen cycling in a natural sod culture apple orchard. Plant Soil Environ., 67: 567–578.


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We evaluated the effects of mowing dominant grasses on root exudation and soil nitrogen (N) cycling by assessing metabolomics analysis of root exudates, microbial metabolism, the abundance of N-cycling-related prokaryotes, and different forms of N concentrations in soil. The treatments included Polygonum aviculare L. mowing (T1), Digitaria sanguinalis (L.) Scop. mowing (T2), and no mowing as the controls (CK1 and CK2). The results showed that compared with the no mowing control (CK1 and CK2), T1 and T2 root exudates contained 223 (178 up-regulated, 45 down-regulated) and 183 (40 up-regulated, 143 down-regulated) differential metabolites, respectively. The average well colour development (AWCD) could reflect the microbial metabolic activity. The AWCD values of T1 were increased while that of T2 decreased on the 2nd day after mowing. The variation in root exudates was the main reason for the change in soil AWCD values and carbon utilisation of T1 and T2 on the 2nd day after mowing. Mowing increased soil microbial biomass N content significantly in the T1 and T2 topsoil. The NO3-N and NH4+-N contents in the 0–10 cm soil increased on the 2nd day after T1 mowing with an increase in the nitrogenase iron protein gene (nifH), glutamate dehydrogenase gene (gdh), ammonia monooxygenase gene (amoA) of ammonia-oxidising archaea (AOA) and ammonia-oxidising bacteria (AOB) abundance. However, NO3-N content decreased on the 2nd day after T2 mowing following a decrease in AOA-amoA and AOB-amoA gene abundance. The results of this study will facilitate the optimisation of sod culture orchard N management, reduction of N fertiliser input, and improvement of N utilisation efficiency.


Ai C., Liang G.Q., Sun J.W., Wang X.B., He P., Zhou W. (2013): Different roles of rhizosphere effect and long-term fertilization in the activity and community structure of ammonia oxidizers in a calcareous fluvo-aquic soil. Soil Biology and Biochemistry, 57: 30–42. https://doi.org/10.1016/j.soilbio.2012.08.003
Carvalhais L.C., Dennis P.G., Badri D.V., Kidd B.N., Vivanco J.M., Schenk P.M. (2015): Linking jasmonic acid signaling, root exudates, and rhizosphere microbiomes. Molecular Plant-Microbe Interactions, 28: 1049–1058. https://doi.org/10.1094/MPMI-01-15-0016-R
Castrillo G., Teixeira P.J.P.L., Paredes S.H., Law T.F., de Lorenzo L., Feltcher M.E., Finkel O.M., Breakfield N.W., Mieczkowski P., Jones C.D., Paz-Ares J., Dangl J.L. (2017): Root microbiota drive direct integration of phosphate stress and immunity. Nature, 543: 513–518. https://doi.org/10.1038/nature21417
Doornbos R.F., van Loon L.C., Bakker P.A.H.M. (2012): Impact of root exudates and plant defense signaling on bacterial communities in the rhizosphere. A review. Agronomy for Sustainable Development, 32: 227–243. https://doi.org/10.1007/s13593-011-0028-y
Eisenhauer N., Dobies T., Cesarz S., Hobbie S.E., Meyer R.J., Worm K., Reich P.B. (2013): Plant diversity effects on soil food webs are stronger than those of elevated CO2 and N deposition in a long-term grassland experiment. Proceedings of the National Academy of Sciences of the United States of America, 110: 6889–6894. https://doi.org/10.1073/pnas.1217382110
Garland J.L. (1997): Analysis and interpretation of community-level physiological profiles in microbial ecology. FEMS Microbiology Ecology, 24: 289–300. https://doi.org/10.1111/j.1574-6941.1997.tb00446.x
Gómez J.A., Guzmán M.G., Giráldez J.V., Fereres E. (2009): The influence of cover crops and tillage on water and sediment yield, and on nutrient, and organic matter losses in an olive orchard on a sandy loam soil. Soil and Tillage Research, 106: 137–144. https://doi.org/10.1016/j.still.2009.04.008
Guitian R., Bardgett R.D. (2000): Plant and soil microbial responses to defoliation in temperate semi-natural grassland. Plant and Soil, 220: 271. https://doi.org/10.1023/A:1004787710886
Hamilton III E.W., Frank D.A., Hinchey P.M., Murray T.R. (2008): Defoliation induces root exudation and triggers positive rhizospheric feedbacks in a temperate grassland. Soil Biology and Biochemistry, 40: 2865–2873. https://doi.org/10.1016/j.soilbio.2008.08.007
Henry S., Texier S., Hallet S., Bru D., Dambreville C., Chèneby D., Bizouard F., Germon J.C., Philippot L. (2008): Disentangling the rhizosphere effect on nitrate reducers and denitrifiers: insight into the role of root exudates. Environmental Microbiology, 10: 3082–3092. https://doi.org/10.1111/j.1462-2920.2008.01599.x
Hokka V., Mikola J., Vestberg M., Setälä H. (2004): Interactive effects of defoliation and an AM fungus on plants and soil organisms in experimental legume-grass communities. Oikos, 106: 73–84. https://doi.org/10.1111/j.0030-1299.2004.12963.x
Huang X.F., Chaparro J.M., Reardon K.F., Zhang R.F., Shen Q.R., Vivanco J.M. (2014): Rhizosphere interactions: root exudates, microbes, and microbial communities. Botany, 92: 267–275. https://doi.org/10.1139/cjb-2013-0225
Kuypers M.M.M., Marchant H.K., Kartal B. (2018): The microbial nitrogen-cycling network. Nature Reviews Microbiology, 16: 263–276. https://doi.org/10.1038/nrmicro.2018.9
Li M.C., Wei G.S., Shi W.C., Sun Z.T., Li H., Wang X.Y., Gao Z. (2018): Distinct distribution patterns of ammonia-oxidizing archaea and bacteria in sediment and water column of the Yellow River estuary. Scientific Reports, 8: 1584. https://doi.org/10.1038/s41598-018-20044-6
Li X.Z., Rui J.P., Xiong J.B., Li J.B., He Z.L., Zhou J.Z., Yannarell A.C., Mackie R.I. (2014): Functional potential of soil microbial communities in the maize rhizosphere. PLoS One, 9: e112609.
Lin X.G. (2010): Principles and Methods of Soil Microbiology Research. Beijing, Beijing Higher Education Press, 73–76.
Paterson E., Thornton B., Midwood A.J., Sim A. (2005): Defoliation alters the relative contributions of recent and non-recent assimilate to root exudation from Festuca rubra. Plant, Cell and Environment, 28: 1525–1533. https://doi.org/10.1111/j.1365-3040.2005.01389.x
Schmidt J.E., Kent A.D., Brisson V.L., Gaudin A.C.M. (2019): Agricultural management and plant selection interactively affect the rhizosphere microbial community structure and nitrogen cycling. Microbiome, 7: 146. https://doi.org/10.1186/s40168-019-0756-9
Schmitt A., Pausch J., Kuzyakov Y. (2013): Effect of clipping and shading on C allocation and fluxes in soil under ryegrass and alfalfa estimated by 14C labeling. Applied Soil Ecology, 64: 228–236. https://doi.org/10.1016/j.apsoil.2012.12.015
Shen X., Yang F., Xiao C.W., Zhou Y. (2020): Increased contribution of root exudates to soil carbon input during grassland degradation. Soil Biology and Biochemistry, 146: 107817. https://doi.org/10.1016/j.soilbio.2020.107817
Steinauer K., Chatzinotas A., Eisenhauer N. (2016): Root exudate cocktails: the link between plant diversity and soil microorganisms? Ecology and Evolution, 6: 7387–7396. https://doi.org/10.1002/ece3.2454
Sun L., Lu Y.F., Yu F.W., Kronzucker H.J., Shi W.M. (2016): Biological nitrification inhibition by rice root exudates and its relationship with nitrogen-use efficiency. New Phytologist, 212: 646–656. https://doi.org/10.1111/nph.14057
Wang C., Zheng M.M., Song W.F., Wen S.L., Wang B., Zhu C., Shen R. (2017): Impact of 25 years of inorganic fertilization on diazotrophic abundance and community structure in acidic soils in southern China. Soil Biology and Biochemistry, 113: 240–249. https://doi.org/10.1016/j.soilbio.2017.06.019
Zhou T.Y., Jiao K.B., Qin S.J., Lyu D.G. (2019): The impact of cover crop shoot decomposition on soil microorganisms in an apple orchard in northeast China. Saudi Journal of Biological Sciences, 26: 1936–1942. https://doi.org/10.1016/j.sjbs.2019.07.004
Zou Q. (2006): Experimental Guidance of Plant Physiology. Beijing, China Agricultural Press.
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