Response of soil phosphatases to glyphosate and its formulations – Roundup (laboratory conditions)  

https://doi.org/10.17221/673/2015-PSECitation:Płatkowski M., Telesiński A. (2016): Response of soil phosphatases to glyphosate and its formulations – Roundup (laboratory conditions)  . Plant Soil Environ., 62: 286-292.
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This paper assesses the impact on certain phosphatase activities in soil of glyphosate and its formulations, i.e.: Roundup 360 SL and Roundup TransEnergy 450 SL, which contain various glyphosate salts (isopropylamine and potassium) and various surfactants (polyethoxylated tallow amine and polyethoxylated ether amine). The experiment was carried out on sandy loam samples with organic carbon content of 10.9 g/kg. Aqueous solutions of pure glyphosate and its formulations were added to the soil. The amounts of applied glyphosate and its salts were: 1, 10 and 100 mg/kg. On days 1, 7, 14, 28 and 56 the activity of alkaline phosphomonoesterase (ALP); acid phosphomonoesterase (ACP); phosphodiesterase (PD); phosphotriesterase (PT) was measured spectrophotometrically. The effect of glyphosate and its formulations depended on the herbicide dosage and day of experiment. ALP and PD were the most susceptible to the presence of glyphosate. A comparison of the impact of glyphosate and its formulations showed that Roundup 360 SL was the most toxic. This could have resulted from the presence of surfactant polyethoxylated tallow amine in formulation. The correlation coefficients and principal component analysis indicated a significant positive relationship between the phosphatase activities in soil containing glyphosate. Significant correlation at P < 0.01 was noted among ALP and ACP, and among ALP and PD. Additionally, at P < 0.05, PD was significantly correlated with ACP, and PT with ALP and with PD.  
References:
Al-Rajab (2014): BEHAVIOR OF THE NON-SELECTIVE HERBICIDE GLYPHOSATE IN AGRICULTURAL SOIL. American Journal of Environmental Sciences, 10, 94-101  https://doi.org/10.3844/ajessp.2014.94.101
 
Browman M. G., Tabatabai M. A. (1978): Phosphodiesterase Activity of Soils1. Soil Science Society of America Journal, 42, 284-  https://doi.org/10.2136/sssaj1978.03615995004200020016x
 
Chaer Guilherme, Fernandes Marcelo, Myrold David, Bottomley Peter (2009): Comparative Resistance and Resilience of Soil Microbial Communities and Enzyme Activities in Adjacent Native Forest and Agricultural Soils. Microbial Ecology, 58, 414-424  https://doi.org/10.1007/s00248-009-9508-x
 
Cherni Ala Edine, Trabelsi Darine, Chebil Samir, Barhoumi Fethi, Rodríguez-Llorente Ignacio D., Zribi Kais (2015): Effect of Glyphosate on Enzymatic Activities, Rhizobiaceae and Total Bacterial Communities in an Agricultural Tunisian Soil. Water, Air, & Soil Pollution, 226, -  https://doi.org/10.1007/s11270-014-2263-8
 
Eivazi F., Tabatabai M.A. (1977): Phosphatases in soils. Soil Biology and Biochemistry, 9, 167-172  https://doi.org/10.1016/0038-0717(77)90070-0
 
Floch Carine, Chevremont Anne-Céline, Joanico Karine, Capowiez Yvan, Criquet Stéven (2011): Indicators of pesticide contamination: Soil enzyme compared to functional diversity of bacterial communities via Biolog® Ecoplates. European Journal of Soil Biology, 47, 256-263  https://doi.org/10.1016/j.ejsobi.2011.05.007
 
Forlani G, Mangiagalli A, Nielsen E, Suardi C.M (1999): Degradation of the phosphonate herbicide glyphosate in soil: evidence for a possible involvement of unculturable microorganisms. Soil Biology and Biochemistry, 31, 991-997  https://doi.org/10.1016/S0038-0717(99)00010-3
 
Franz J.E., Mao M.K., Sikorski J.A. (1997): Glyphosate: A Unique Global Herbicide. Washington, American Chemical Society.
 
Gomes M. P., Smedbol E., Chalifour A., Henault-Ethier L., Labrecque M., Lepage L., Lucotte M., Juneau P. (): Alteration of plant physiology by glyphosate and its by-product aminomethylphosphonic acid: an overview. Journal of Experimental Botany, , -  https://doi.org/10.1093/jxb/eru269
 
Haney R. L., Senseman S. A., Hons F. M., Zuberer D. A. (2000): Effect of glyphosate on soil microbial activity and biomass. Weed Science, 48, 89-93  https://doi.org/10.1614/0043-1745(2000)048[0089:EOGOSM]2.0.CO;2
 
Howe Christina M., Berrill Michael, Pauli Bruce D., Helbing Caren C., Werry Kate, Veldhoen Nik (2004): TOXICITY OF GLYPHOSATE-BASED PESTICIDES TO FOUR NORTH AMERICAN FROG SPECIES. Environmental Toxicology and Chemistry, 23, 1928-  https://doi.org/10.1897/03-71
 
Krzysko-Lupicka Teresa, Sudol Teresa (2008): Interactions between glyphosate and autochthonous soil fungi surviving in aqueous solution of glyphosate. Chemosphere, 71, 1386-1391  https://doi.org/10.1016/j.chemosphere.2007.11.006
 
Lane Matthew, Lorenz Nicola, Saxena Jyotisna, Ramsier Cliff, Dick Richard P. (2012): The effect of glyphosate on soil microbial activity, microbial community structure, and soil potassium. Pedobiologia, 55, 335-342  https://doi.org/10.1016/j.pedobi.2012.08.001
 
Moore Lindsay J., Fuentes Latice, Rodgers John H., Bowerman William W., Yarrow Greg K., Chao Wayne Y., Bridges William C. (2012): Relative toxicity of the components of the original formulation of Roundup® to five North American anurans. Ecotoxicology and Environmental Safety, 78, 128-133  https://doi.org/10.1016/j.ecoenv.2011.11.025
 
Nakatani André Shigueyoshi, Fernandes Marcelo Ferreira, de Souza Rosinei Aparecida, da Silva Adriana Pereira, dos Reis-Junior Fábio Bueno, Mendes Iêda Carvalho, Hungria Mariangela (2014): Effects of the glyphosate-resistance gene and of herbicides applied to the soybean crop on soil microbial biomass and enzymes. Field Crops Research, 162, 20-29  https://doi.org/10.1016/j.fcr.2014.03.010
 
Płatkowski M., Telesiński A. (2015a): Effect of different glyphosate salts on phosphodiesterase and phosphotriesterase activities in soil with reference to ecological importance of soil pollution. A laboratory experiment. Environmental Protection and Natural Resources, 26: 9–14.
 
Płatkowski M., Telesiński A. (2015b): Effects of glyphosate ammonium salt on the bioavailable phosphorus content and the activity of selected phosphatases in loamy sand. Inżynieria Ekologiczna, 43: 115–121. (In Polish)
 
Sannino F, Gianfreda L (2001): Pesticide influence on soil enzymatic activities. Chemosphere, 45, 417-425  https://doi.org/10.1016/S0045-6535(01)00045-5
 
Sihtmäe M., Blinova I., Künnis-Beres K., Kanarbik L., Heinlaan M., Kahru A. (2013): Ecotoxicological effects of different glyphosate formulations. Applied Soil Ecology, 72, 215-224  https://doi.org/10.1016/j.apsoil.2013.07.005
 
Sudoł T., Krzyśko-Łupicka T. (2005): Direct indicators of determination of glyphosate decomposition by filamentous fungi. Physicochemical Problems of Mineral Processing, 39: 257–261.
 
Tabatabai M.A., Bremner J.M. (1969): Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry, 1, 301-307  https://doi.org/10.1016/0038-0717(69)90012-1
 
Tejada Manuel (2009): Evolution of soil biological properties after addition of glyphosate, diflufenican and glyphosate+diflufenican herbicides. Chemosphere, 76, 365-373  https://doi.org/10.1016/j.chemosphere.2009.03.040
 
Uren Webster Tamsyn M., Laing Lauren V., Florance Hannah, Santos Eduarda M. (2014): Effects of Glyphosate and its Formulation, Roundup, on Reproduction in Zebrafish ( Danio rerio ). Environmental Science & Technology, 48, 1271-1279  https://doi.org/10.1021/es404258h
 
Vereecken Harry (2005): Mobility and leaching of glyphosate: a review. Pest Management Science, 61, 1139-1151  https://doi.org/10.1002/ps.1122
 
Wang J.B., Chen Z.H., Chen L.J., Zhu A.N., Wu Z.J. (2011): Surface soil phosphorus and phosphatase activities affected by tillage and crop residue input amounts. Plant, Soil and Environment, 57: 251–257.
 
Yu Ying, Zhang Haijun, Zhou Qixing (2011): Using Soil Available P and Activities of Soil Dehydrogenase and Phosphatase as Indicators for Biodegradation of Organophosphorus Pesticide Methamidophos and Glyphosate. Soil and Sediment Contamination: An International Journal, 20, 688-701  https://doi.org/10.1080/15320383.2011.594110
 
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