Blue lupine seeds protein content and amino acids composition A., Zielinska-Dawidziak M., Piasecka-Kwiatkowska D., Lampart-Szczapa E. (2018): Blue lupine seeds protein content and amino acids composition. Plant Soil Environ., 64: 147-155.
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Lupine seeds are promising soy replacement in food products and feeds. Eighteen cultivars of blue lupine seeds were examined to indicate the one most favourable in protein content and quality. Two parameters were studied, i.e. total protein content (with the Kjeldahl method) and amino acids composition (with the ultra performance liquid chromatography technique). Both parameters studied were variable and depended both on the cultivar and on the place of cultivation. Protein content was in the range of 28–41% and the worst cultivar, regardless of cultivation place, was cv. Kalif (average total protein content 29.37 ± 1.14%), while the best cultivar was Boruta (average total protein content 37.43 ± 0.98%). The blue lupine seeds were rich especially in leucine (5.3 ± 0.5–9.7 ± 0.5 g/16 g N), threonine (2.4 ± 0.7–4.9 ± 0.1 g/16 g N) and lysine (2.7 ± 0.4–5.6 ± 0.1 g/16 g N). The richest among all amino acids studied were the cv. Bojar seeds from Wiatrowo and cv. Oskar from Przebędowo. It was not possible to choose one cultivar preferable from the nutritional point of view, because a strong influence of cultivation place on protein content and quality was observed, especially as to the precipitation sum.

Adomas B., Piotrowicz-Cieślak A.I., Kowalik K. (2005): Lupine seeds biological properties. Postępy Nauk Rolniczych, 5: 54–63. (In Polish)
AOAC International (2014): Standard Method Performance Requirements for Determination of Amino Acids in Infant Formula and Adult/Pediatric Nutritional Formula. Available at:
Bouchoucha R., Fradj M.K.B., Bouchoucha M., Akrout M., Feki M., Kaabachi N., Raies A., Slimane H. (2016): Effect of Lupinus albus on glycaemic control, plasma insulin levels, lipid profile and liver enzymes in type 2 diabetics. Journal of Food and Nutrition Research, 4: 615–620.
COBOR (2015): Available at:
Department of Agriculture and Food, Government of West Australia (2016): Available at:
FAO (2003): Food and Agriculture Organization Corporate Document Repository. Rome, 7–11. Available at:
FAO/WHO (2007): Food and Agriculture Organization Protein and Amino Acid Requirements in Human Nutrition. (Technical Report Series 935). Available at:
Fontanari Gustavo Guadagnucci, Batistuti José Paschoal, Cruz Robison José da, Saldiva Paulo Hilário Nascimento, Arêas José Alfredo Gomes (2012): Cholesterol-lowering effect of whole lupin (Lupinus albus) seed and its protein isolate. Food Chemistry, 132, 1521-1526
Khalid Ikhlas, Elhardallou Sirelhatim, Gobouri Adil (2016): Amino Acid Composition and Physicochemical Properties of Bitter Lupine (Lupinustermis) Seed Flour. Oriental Journal of Chemistry, 32, 3175-3182
Lizarazo Clara I, Lampi Anna-Maija, Liu Jingwei, Sontag-Strohm Tuula, Piironen Vieno, Stoddard Frederick L (2015): Nutritive quality and protein production from grain legumes in a boreal climate. Journal of the Science of Food and Agriculture, 95, 2053-2064
Millán-Linares Mª del Carmen, Bermúdez Beatriz, Yust María del Mar, Millán Francisco, Pedroche Justo (2014): Anti-inflammatory activity of lupine (Lupinus angustifolius L.) protein hydrolysates in THP-1-derived macrophages. Journal of Functional Foods, 8, 224-233
Mahmoud Eman A.M., Nassef Shereen L., Basuny Amany M.M. (2012): Production of high protein quality noodles using wheat flour fortified with different protein products from lupine. Annals of Agricultural Sciences, 57, 105-112
Regina Pereira Monteiro Márcia, Bárbara Pereira Costa Aline, Fabiane Campos Suellen, Ramalho Silva Mauro, Oliveira da Silva Cassiano, Stampini Duarte Martino Hércia, Pinto Coelho Silvestre Marialice (2014): Evaluation of the chemical composition, protein quality and digestibility of lupin (Lupinus albus and Lupinus angustifolius). O Mundo da Saúde, 38, 251-259
Pisarikova B., Zraly Z., Bunka F., Trckova M. (2008): Nutritional value of white lupine cultivar Butan in diets for fattening pigs. Veterinární Medicína, 53, 124-134
Podleśny J., Podleśna A. (2011): Effect of rainfall amount and distribution on growth, development and yields of determinate and indeterminate cultivars of blue lupin. Polish Journal of Agronomy, 4: 16–22.
Sobotka Wiesław, Stanek Maria, Bogusz Jacek (2016): Evaluation of the Nutritional Value of Yellow (Lupinus Luteus) and Blue Lupine (Lupinus Angustifolius) Cultivars as Protein Sources in Rats. Annals of Animal Science, 16, -
Sońta M., Rekiel A. (2016): Legumes production and use for fodder purposes. Part I. Legumes production in Poland and in the world. Breeding Review, 5: 30–34. (In Polish)
Stanek M., Bogusz J., Sobotka W., Bieniaszewski T. (2012): Nutritional value of Lupinus angustifolius seeds. Fragmenta Agronomica, 29: 160–166. (In Polish)
Sujak Agnieszka, Kotlarz Anna, Strobel Wacław (2006): Compositional and nutritional evaluation of several lupin seeds. Food Chemistry, 98, 711-719
U.S. National Library of Medicine (2015): Available at:
Zielińska-Dawidziak M., Nawracała J., Piasecka-Kwiatkowska D., Król E., Staniek H., Krejpcio Z. (2012): The impact of the year of soybean (Glycine max L. Merrill) harvest on the accumulation of iron from FeSO4 solutions. Fragmenta Agronomica, 29: 183–193.
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