Irreplaceability of phosphorus as a necessary macroelement in crop production is due to limited resources and costly processing of ores and immobilization in soil, which force for seeking an alternative sources or the use of waste materials. In this paper, the waste aluminum phosphate from pharmaceutical factory used as phosphate fertilizer and its effects were compared with other phosphorus fertilizers (superphosphate and rock phosphate). Except the analysis of available phosphorus (AL-method) the sequential extraction of phosphorus (modified Chang and Jackson) and sequential extraction of aluminum (modified Tessier) were performed. The experimental plant was mustard (Sinapis alba). The pot experiment was carried out on two soil types: Stagnosol and Vertisol. Application of phosphorus with aluminum phosphate had the same effect as the application of other phosphatic fertilizers in both soil types. In Stagnosol Al-phosphate directly influenced the increase in plant fresh weight by 39% and dry weight by 43% compared to the control, and also decreased the content of mobile Al for 40% and Pb for 47% in plant biomass. Based on these results, the use of waste aluminum phosphate has a potential to be used as a phosphorus fertilizer under given conditions.