Variation of glucosinolates concentration and root growth of horseradish as affected by nitrogen and sulphur supply
The aim of the study was to evaluate total and individual glucosinolates concentration, root weight and diameters of horseradish accessions (Cor and Mon) grown by adding nitrogen (N) and sulphur (S) and harvested at the beginning (late autumn) and towards the end (early spring) of the harvest period. The concentration of the 9 glucosinolates (GLS) quantified in roots varied greatly between accessions (6-fold higher in Cor) and with fertilization (N alone led to an increase of 64% of GLS with an additional enhancement of 65% when S was added to N). Root GLS concentration was significantly higher in early spring compared to the previous harvest in late autumn (12.5 vs 8.9 µmol/g DW (dry weight)); a drastic decline of GLS (2.7 µmol/g DW) occurred in root harvested in the following autumn from plants left ad hoc in the field. The aliphatic sinigrin was the most abundant (80% of total GLS), followed by the aromatic gluconasturtiin and the indolic glucobrassicin (5.8% and 4.7%, respectively). Root diameter and weight increased in plants treated with fertilizers (19% and 61%, respectively) and throughout the harvests with Cor showing values double with respect to Mon (925 vs 476 g/plant). Relationships between GLS concentration and root weight (R2 = 0.61) and diameter (R2 = 0.69) were improved by excluding the roots harvested from plants left ad hoc in the field that showed the worst performances.
Agneta R., Lelario F., De Maria S., Möllers C., Bufo S.A., Rivelli A.R. (2014a): Glucosinolate profile and distribution among plant tissues and phenological stages of field-grown horseradish. Phytochemistry, 106: 178–187.
Agneta R., Möllers C., De Maria S., Rivelli A.R. (2014b): Evaluation of root yield traits and glucosinolate concentration of different Armoracia rusticana accessions in Basilicata region (southern Italy). Scientia Horticulturae, 170: 249–255.
Agneta Rosa, Möllers Christian, Rivelli Anna Rita (2013): Horseradish (Armoracia rusticana), a neglected medical and condiment species with a relevant glucosinolate profile: a review. Genetic Resources and Crop Evolution, 60, 1923-1943
https://doi.org/10.1007/s10722-013-0010-4
Alnsour M., Kleinwächter M., Böhme J., Selmar D. (2012): Sulfate determines the glucosinolate concentration of horseradish in vitro plants (Armoracia rusticana Gaertn., Mey. & Scherb.). Journal of the Science of Food and Agriculture, 93: 918–923.
Björkman Maria, Klingen Ingeborg, Birch Andrew N.E., Bones Atle M., Bruce Toby J.A., Johansen Tor J., Meadow Richard, Mølmann Jørgen, Seljåsen Randi, Smart Lesley E., Stewart Derek (2011): Phytochemicals of Brassicaceae in plant protection and human health – Influences of climate, environment and agronomic practice. Phytochemistry, 72, 538-556
https://doi.org/10.1016/j.phytochem.2011.01.014
De Maria Susanna, Agneta Rosa, Lelario Filomena, Möllers Christian, Rivelli Anna Rita (2016): Influence of nitrogen and sulfur fertilization on glucosinolate content and composition of horseradish plants harvested at different developmental stages. Acta Physiologiae Plantarum, 38, -
https://doi.org/10.1007/s11738-016-2110-1
Falk K. L., Tokuhisa J. G., Gershenzon J. (2007): The Effect of Sulfur Nutrition on Plant Glucosinolate Content: Physiology and Molecular Mechanisms. Plant Biology, 9, 573-581
https://doi.org/10.1055/s-2007-965431
Food and Drug Administration (2008): Everything Added to Food in the United States (EAFUS) Available at: http://www.fda.gov/Food/FoodIngredientsPackaging/ucm115326.htm
Ku Kang-Mo, Jeffery Elizabeth H., Juvik John A., Kushad Mosbah M. (2015): Correlation of Quinone Reductase Activity and Allyl Isothiocyanate Formation Among Different Genotypes and Grades of Horseradish Roots. Journal of Agricultural and Food Chemistry, 63, 2947-2955
https://doi.org/10.1021/jf505591z
Lelario Filomena, De Maria Susanna, Agneta Rosa, M?llers Christian, Bufo Sabino, Rivelli Anna (2015): Glucosinolates Determination in Tissues of Horseradish Plant. BIO-PROTOCOL, 5, -
https://doi.org/10.21769/BioProtoc.1562
Li Xian, Kushad Mosbah M. (2004): Correlation of Glucosinolate Content to Myrosinase Activity in Horseradish (
Armoracia rusticana ). Journal of Agricultural and Food Chemistry, 52, 6950-6955
https://doi.org/10.1021/jf0401827
Nguyen Nhat Minh, Gonda Sándor, Vasas Gábor (2013): A Review on the Phytochemical Composition and Potential Medicinal Uses of Horseradish (
Armoracia rusticana ) Root. Food Reviews International, 29, 261-275
https://doi.org/10.1080/87559129.2013.790047
Perlaki Z., Djurovka M. (2009): Horseradish root yield depending on organic and mineral fertilizers. Contemporary Agriculture, 58: 106–111.
Sultana Tamanna, Savage Geoffrey P, McNeil David L, Porter Noel G, Martin Richard J, Deo Bas (2002): Effects of fertilisation on the allyl isothiocyanate profile of above-ground tissues of New Zealand-grown wasabi. Journal of the Science of Food and Agriculture, 82, 1477-1482
https://doi.org/10.1002/jsfa.1218
UPOV International Union for the Protection of New Varieties of Plants (2001): Guidelines for the Conduct of Tests for Distinctness, Uniformity and Stability. Horseradish (Armoracia rusticana Gaertn, Mey. & Scherb.). Geneva, TG/191/2.
Velasco Pablo, Cartea María Elena, González Carmen, Vilar Marta, Ordás Amando (2007): Factors Affecting the Glucosinolate Content of Kale (
Brassica oleraceaacephala Group). Journal of Agricultural and Food Chemistry, 55, 955-962
https://doi.org/10.1021/jf0624897
Walters S.A., Wahle E.A. (2010): Horseradish production in Illinois. HortTechnology, 20: 267–276.
Wedelsbäck Bladh K. (2014): Biodiversity in nordic horseradish (Armoracia rusticana). [Ph.D. Thesis] Alnarp, Swedish University of Agricultural Sciences.
Zukalová H., Vašák J. (2002): The role and effects of glucosinolates of Brassica species – A review. Rostlinná Výroba, 48: 175–180.
