High-methionine soybean has no significant effect on nitrogen-transforming bacteria in rhizosphere soil

https://doi.org/10.17221/750/2017-PSECitation:Liang J., Luan Y., Jiao Y., Sun S., Wu C., Wu H., Zhang M., Zhang H., Zheng X., Zhang Z. (2018): High-methionine soybean has no significant effect on nitrogen-transforming bacteria in rhizosphere soil. Plant Soil Environ., 64: 108-113.
download PDF

Transgenic plants may induce shifts in the microbial community composition that in turn alter microbially-mediated nutrient cycling in soil. Studies of how specific microbial groups respond to genetically modified (GM) planting help predict potential impacts upon processes performed by these groups. This study investigated the effect of transgenic high-methionine soybean cv. ZD91 on nitrogen-fixing and ammonia-oxidizing bacterial populations. A difference in nitrogen-fixing or ammonia-oxidizing bacteria community composition was not found, suggesting that cv. ZD91 does not alter the bacterial populations in rhizosphere soil. This study increases our understanding of the potential effect of transgenic soybean on microbial functional groups within soil by suggesting that nitrogen-transforming bacteria may be useful for future investigations on the GM crops impact in the soil ecosystem.

Alguacil Maria del Mar, Torrecillas Emma, Hernández Guillermina, Roldán Antonio, Moora Mari (2012): Changes in the Diversity of Soil Arbuscular Mycorrhizal Fungi after Cultivation for Biofuel Production in a Guantanamo (Cuba) Tropical System. PLoS ONE, 7, e34887-  https://doi.org/10.1371/journal.pone.0034887
ANDOW DAVID A., HILBECK ANGELIKA (2004): Science-Based Risk Assessment for Nontarget Effects of Transgenic Crops. BioScience, 54, 637-  https://doi.org/10.1641/0006-3568(2004)054[0637:SRAFNE]2.0.CO;2
Bannert Andrea, Kleineidam Kristina, Wissing Livia, Mueller-Niggemann Cornelia, Vogelsang Vanessa, Welzl Gerhard, Cao Zhihong, Schloter Michael (2011): Changes in Diversity and Functional Gene Abundances of Microbial Communities Involved in Nitrogen Fixation, Nitrification, and Denitrification in a Tidal Wetland versus Paddy Soils Cultivated for Different Time Periods. Applied and Environmental Microbiology, 77, 6109-6116  https://doi.org/10.1128/AEM.01751-10
Caporaso J Gregory, Kuczynski Justin, Stombaugh Jesse, Bittinger Kyle, Bushman Frederic D, Costello Elizabeth K, Fierer Noah, Peña Antonio Gonzalez, Goodrich Julia K, Gordon Jeffrey I, Huttley Gavin A, Kelley Scott T, Knights Dan, Koenig Jeremy E, Ley Ruth E, Lozupone Catherine A, McDonald Daniel, Muegge Brian D, Pirrung Meg, Reeder Jens, Sevinsky Joel R, Turnbaugh Peter J, Walters William A, Widmann Jeremy, Yatsunenko Tanya, Zaneveld Jesse, Knight Rob (2010): QIIME allows analysis of high-throughput community sequencing data. Nature Methods, 7, 335-336  https://doi.org/10.1038/nmeth.f.303
Cotta Simone Raposo, Dias Armando Cavalcante Franco, Marriel Ivanildo Evódio, Andreote Fernando Dini, Seldin Lucy, van Elsas Jan Dirk, Schottel J. L. (2014): Different Effects of Transgenic Maize and Nontransgenic Maize on Nitrogen-Transforming Archaea and Bacteria in Tropical Soils. Applied and Environmental Microbiology, 80, 6437-6445  https://doi.org/10.1128/AEM.01778-14
García Araceli Nora, Ayub Nicolás Daniel, Fox Ana Romina, Gómez María Cristina, Diéguez María José, Pagano Elba María, Berini Carolina Andrea, Muschietti Jorge Prometeo, Soto Gabriela (2014): Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia. BMC Plant Biology, 14, -  https://doi.org/10.1186/s12870-014-0248-9
Gong Chun Yan, Wang Tai (2013): Proteomic evaluation of genetically modified crops: current status and challenges. Frontiers in Plant Science, 4, -  https://doi.org/10.3389/fpls.2013.00041
Hai B. (2009): Impact of environmental factors on key functional groups involved in nitrogen cycling in agricultural ecosystems. [Ph.D. thesis] Munich, Ludwig-Maximilian University of Munich, 62.
Hai B., Diallo N. H., Sall S., Haesler F., Schauss K., Bonzi M., Assigbetse K., Chotte J.-L., Munch J. C., Schloter M. (2009): Quantification of Key Genes Steering the Microbial Nitrogen Cycle in the Rhizosphere of Sorghum Cultivars in Tropical Agroecosystems. Applied and Environmental Microbiology, 75, 4993-5000  https://doi.org/10.1128/AEM.02917-08
Hayden Helen L., Drake Judy, Imhof Mark, Oxley Andrew P.A., Norng Sorn, Mele Pauline M. (2010): The abundance of nitrogen cycle genes amoA and nifH depends on land-uses and soil types in South-Eastern Australia. Soil Biology and Biochemistry, 42, 1774-1783  https://doi.org/10.1016/j.soilbio.2010.06.015
Ikeda Seishi, Omura Toshikazu, Ytow Nozomi, Komaki Hisayuki, Minamisawa Kiwamu, Ezura Hiroshi, Fujimura Tatsuhito (2006): Microbial Community Analysis in the Rhizosphere of a Transgenic Tomato that Overexpresses 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase. Microbes and Environments, 21, 261-271  https://doi.org/10.1264/jsme2.21.261
ISAAA (2016): Global Status of Commercialized Biotech/GM Crops: 2016. ISAAA Brief No. 52. Ithaca, International Service for the Acquisition of Agri-biotech Applications.
Knox Oliver G. G., Gupta Vadakattu V. S. R., Lardner Richard (2014): Field evaluation of the effects of cotton variety and GM status on rhizosphere microbial diversity and function in Australian soils. Soil Research, 52, 203-  https://doi.org/10.1071/SR12361
Lamarche J., Hamelin R. C. (2007): No Evidence of an Impact on the Rhizosphere Diazotroph Community by the Expression of Bacillus thuringiensis Cry1Ab Toxin by Bt White Spruce. Applied and Environmental Microbiology, 73, 6577-6583  https://doi.org/10.1128/AEM.00812-07
Levy-Booth David J., Prescott Cindy E., Grayston Susan J. (2014): Microbial functional genes involved in nitrogen fixation, nitrification and denitrification in forest ecosystems. Soil Biology and Biochemistry, 75, 11-25  https://doi.org/10.1016/j.soilbio.2014.03.021
Liang Jingang, Meng Fang, Sun Shi, Wu Cunxiang, Wu Haiying, Zhang Mingrong, Zhang Haifeng, Zheng Xiaobo, Song Xinyuan, Zhang Zhengguang, Aroca Ricardo (2015): Community Structure of Arbuscular Mycorrhizal Fungi in Rhizospheric Soil of a Transgenic High-Methionine Soybean and a Near Isogenic Variety. PLOS ONE, 10, e0145001-  https://doi.org/10.1371/journal.pone.0145001
Liang Jingang, Sun Shi, Ji Jun, Wu Haiying, Meng Fang, Zhang Mingrong, Zheng Xiaobo, Wu Cunxiang, Zhang Zhengguang, Balestrini Raffaella (2014): Comparison of the Rhizosphere Bacterial Communities of Zigongdongdou Soybean and a High-Methionine Transgenic Line of This Cultivar. PLoS ONE, 9, e103343-  https://doi.org/10.1371/journal.pone.0103343
JG Liang, LT Xin, Meng F., Sun S., CX Wu, HY Wu, MR Zhang, HF Zhang, XB Zheng, ZG Zhang (2016): High-methionine soybean has no adverse effect on functional diversity of rhizosphere microorganisms  . Plant, Soil and Environment, 62, 441-446  https://doi.org/10.17221/241/2016-PSE
Martinelli Lucia, Karbarz Małgorzata, Siipi Helena (2013): Science, safety, and trust: the case of transgenic food. Croatian Medical Journal, 54, 91-96  https://doi.org/10.3325/cmj.2013.54.91
Nelson Darryl R., Mele Pauline M. (2007): Subtle changes in rhizosphere microbial community structure in response to increased boron and sodium chloride concentrations. Soil Biology and Biochemistry, 39, 340-351  https://doi.org/10.1016/j.soilbio.2006.08.004
Ollivier Julien, Töwe Stefanie, Bannert Andrea, Hai Brigitte, Kastl Eva-Maria, Meyer Annabel, Su Ming Xia, Kleineidam Kristina, Schloter Michael (2011): Nitrogen turnover in soil and global change. FEMS Microbiology Ecology, 78, 3-16  https://doi.org/10.1111/j.1574-6941.2011.01165.x
Rosch C., Mergel A., Bothe H. (2002): Biodiversity of Denitrifying and Dinitrogen-Fixing Bacteria in an Acid Forest Soil. Applied and Environmental Microbiology, 68, 3818-3829  https://doi.org/10.1128/AEM.68.8.3818-3829.2002
Rotthauwe J.H., Witzel K.P., Liesack W. (1997): The ammonia monooxygenase structural gene amoA as a functional marker: Molecular fine-scale analysis of natural ammonia-oxidizing populations. Applied and Environmental Microbiology, 63: 4704–4712.
Song Shikui, Hou Wensheng, Godo Itamar, Wu Cunxiang, Yu Yang, Matityahu Ifat, Hacham Yael, Sun Shi, Han Tianfu, Amir Rachel (2013): Soybean seeds expressing feedback-insensitive cystathionine γ-synthase exhibit a higher content of methionine. Journal of Experimental Botany, 64, 1917-1926  https://doi.org/10.1093/jxb/ert053
Stewart Katherine J., Grogan Paul, Coxson Darwyn S., Siciliano Steven D. (2014): Topography as a key factor driving atmospheric nitrogen exchanges in arctic terrestrial ecosystems. Soil Biology and Biochemistry, 70, 96-112  https://doi.org/10.1016/j.soilbio.2013.12.005
Turrini Alessandra, Sbrana Cristiana, Giovannetti Manuela (2015): Belowground environmental effects of transgenic crops: a soil microbial perspective. Research in Microbiology, 166, 121-131  https://doi.org/10.1016/j.resmic.2015.02.006
Zadorina E. V., Boulygina E. S., Kolganova T. V., Kuznetsov B. B., Skryabin K. G. (2009): Evaluation of the effect of late blight-resistant potato plants on the structure of bacterial associations in soil. Applied Biochemistry and Microbiology, 45, 194-198  https://doi.org/10.1134/S0003683809020136
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti