Effects of 3,4-dimethylpyrazole phosphate and dicyandiamide on nitrous oxide emission in a greenhouse vegetable soil

https://doi.org/10.17221/762/2014-PSECitation:Kou Y.P., Wei K., Chen G.X., Wang Z.Y., Xu H. (2015): Effects of 3,4-dimethylpyrazole phosphate and dicyandiamide on nitrous oxide emission in a greenhouse vegetable soil. Plant Soil Environ., 61: 29-35.
download PDF

A laboratory study was conducted to determine the effect of 3,4-dimethylpyrazole phosphate (DMPP) and dicyandiamide (DCD) on nitrous oxide (N2O) emission, mineral nitrogen (NH4+-N, NO3-N) contents, as well as ammonia oxidizing and denitrifying microbes in a greenhouse vegetable soil. Five treatments were established at 55% and 75% water filled pore space (WFPS): no fertilizer; urea; urea + manure (UM); urea + manure + DCD (UMDCD) and urea + manure + DMPP (UMDMPP). The application rate of urea and manure was 1800 kg N/ha and 1000 kg N/ha, respectively. DMPP and DCD were applied at the rate of 0.5% and 2% of urea-N application rate, respectively. Compared to UMDCD treatment, UMDMPP treatment significantly reduced N2O emission and NO3-N content and delayed ammonia oxidation, and showed a stronger inhibition effect on ammonium-oxidizing bacteria at both WFPS. Moreover, the copy numbers of nirS and nirK genes decreased significantly in the presence of DMPP at both WFPS, but were not affected by DCD. These results suggest that the application of DMPP is more effective than DCD on N2O mitigation in high N level vegetable soil, although the application rate of DMPP was one quarter that of DCD.

Bao Qiongli, Ju Xiaotang, Gao Bing, Qu Zhi, Christie Peter, Lu Yahai (2012): Response of Nitrous Oxide and Corresponding Bacteria to Managements in an Agricultural Soil. Soil Science Society of America Journal, 76, 130-  https://doi.org/10.2136/sssaj2011.0152
G. Barth, Tucher S. von, U. Schmidhalter (2001): Influence of soil parameters on the effect of 3,4-dimethylpyrazole-phosphate as a nitrification inhibitor. Biology and Fertility of Soils, 34, 98-102  https://doi.org/10.1007/s003740100382
Braker G., Fesefeldt A., Witzel K.P. (1998): Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples. Applied and Environmental Microbiology, 64: 3769–3775.
Chen Deli, Suter Helen C., Islam Arshad, Edis Robert (2010): Influence of nitrification inhibitors on nitrification and nitrous oxide (N2O) emission from a clay loam soil fertilized with urea. Soil Biology and Biochemistry, 42, 660-664  https://doi.org/10.1016/j.soilbio.2009.12.014
Chen Qing, Zhang Xiaosheng, Zhang Hongyan, Christie Peter, Li Xiaolin, Horlacher Dieter, Liebig Hans-Peter (2004): Evaluation of current fertilizer practice and soil fertility in vegetable production in the Beijing region. Nutrient Cycling in Agroecosystems, 69, 51-58  https://doi.org/10.1023/B:FRES.0000025293.99199.ff
Di Hong Jie, Cameron Keith C. (2011): Inhibition of ammonium oxidation by a liquid formulation of 3,4-Dimethylpyrazole phosphate (DMPP) compared with a dicyandiamide (DCD) solution in six new Zealand grazed grassland soils. Journal of Soils and Sediments, 11, 1032-1039  https://doi.org/10.1007/s11368-011-0372-1
Di H.J., Cameron K.C. (2012): How does the application of different nitrification inhibitors affect nitrous oxide emissions and nitrate leaching from cow urine in grazed pastures? Soil Use and Management, 28: 54–61.
Dobbie K. E., McTaggart I. P., Smith K. A. (1999): Nitrous oxide emissions from intensive agricultural systems: Variations between crops and seasons, key driving variables, and mean emission factors. Journal of Geophysical Research, 104, 26891-  https://doi.org/10.1029/1999JD900378
Dong X.X., Zhang L.L., Wu Z.J., Zhang H.W., Gong P. (2013): The response of nitrifier, N-fixer and denitrifier gene copy numbers to the nitrification inhibitor 3,4-dimethylpyrazole phosphate. Plant, Soil and Environment, 59: 398–403.
Erguder Tuba H., Boon Nico, Wittebolle Lieven, Marzorati Massimo, Verstraete Willy (2009): Environmental factors shaping the ecological niches of ammonia-oxidizing archaea. FEMS Microbiology Reviews, 33, 855-869  https://doi.org/10.1111/j.1574-6976.2009.00179.x
Francis C. A., Roberts K. J., Beman J. M., Santoro A. E., Oakley B. B. (): Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proceedings of the National Academy of Sciences, 102, 14683-14688  https://doi.org/10.1073/pnas.0506625102
Garbeva Paolina, Baggs Elizabeth M., Prosser James I. (2007): Phylogeny of nitrite reductase ( nirK ) and nitric oxide reductase ( norB ) genes from Nitrosospira species isolated from soil. FEMS Microbiology Letters, 266, 83-89  https://doi.org/10.1111/j.1574-6968.2006.00517.x
GONG Ping, ZHANG Li-Li, WU Zhi-Jie, CHEN Zhen-Hua, CHEN Li-Jun (2013): Responses of Ammonia-Oxidizing Bacteria and Archaea in Two Agricultural Soils to Nitrification Inhibitors DCD and DMPP: A Pot Experiment. Pedosphere, 23, 729-739  https://doi.org/10.1016/S1002-0160(13)60065-X
Jha N., Deslippe J., Saggar S., Tillman R., Giltrap D. (2013): Measuring bacterial denitrifier genes distribution and abundance in New Zealand dairy-grazed pasture soils. Accurate and efficient use of nutrients on farms. Occasional Report.
Ju X.T., Liu X.J., Zhang F.S., Roelcke M. (2004): Nitrogen fertilization, soil nitrate accumulation, and policy recommendations in several agricultural regions of China. Ambio: A Journal of the Human Environment, 33: 300–305.
Kowalchuk George A., Stephen John R. (2001): A MMONIA- O XIDIZING B ACTERIA : A Model for Molecular Microbial Ecology. Annual Review of Microbiology, 55, 485-529  https://doi.org/10.1146/annurev.micro.55.1.485
Menéndez Sergio, Barrena Iskander, Setien Igor, González-Murua Carmen, Estavillo José María (2012): Efficiency of nitrification inhibitor DMPP to reduce nitrous oxide emissions under different temperature and moisture conditions. Soil Biology and Biochemistry, 53, 82-89  https://doi.org/10.1016/j.soilbio.2012.04.026
Miller Michelle N., Dandie Catherine E., Zebarth Bernie J., Burton David L., Goyer Claudia, Trevors Jack T. (2012): Influence of carbon amendments on soil denitrifier abundance in soil microcosms. Geoderma, 170, 48-55  https://doi.org/10.1016/j.geoderma.2011.11.022
Nicol Graeme W., Leininger Sven, Schleper Christa, Prosser James I. (2008): The influence of soil pH on the diversity, abundance and transcriptional activity of ammonia oxidizing archaea and bacteria. Environmental Microbiology, 10, 2966-2978  https://doi.org/10.1111/j.1462-2920.2008.01701.x
Rotthauwe J.H., Witzel K.P., Liesack W. (1997): The ammonia monooxygenase structural gene amoA as a functional marker: Molecular fine-scale analysis of natural ammonia-oxidizing populations. Applied and Environmental Microbiology, 63: 4704–4712.
Throbäck Ingela Noredal, Enwall Karin, Jarvis ÅSa, Hallin Sara (2004): Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE. FEMS Microbiology Ecology, 49, 401-417  https://doi.org/10.1016/j.femsec.2004.04.011
A. Weiske, G. Benckiser, T. Herbert, J. Ottow (2001): Influence of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) in comparison to dicyandiamide (DCD) on nitrous oxide emissions, carbon dioxide fluxes and methane oxidation during 3 years of repeated application in field experiments. Biology and Fertility of Soils, 34, 109-117  https://doi.org/10.1007/s003740100386
Zerulla W., Barth T., Dressel J., Erhardt K., von Locquenghien K.H., Pasda G., Radle M., Wissemeier A.H. (2001): 3,4-Dime-
thylpyrazole phosphate (DMPP) – A new nitrification inhibitor for agriculture and horticulture – An introduction. Biology and Fertility of Soils, 34: 79–84.
Zheng X.H., Han S.H., Huang Y., Wang Y.S., Wang M.X. (2004): Re-quantifying the emission factors based on field measurements and estimating the direct N2O emission from Chinese croplands. Global Biogeochemical Cycles, 18.
download PDF

© 2020 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti