Dynamics of microbial population size in rhizosphere soil of Monsanto’s Cry1Ac cotton

https://doi.org/10.17221/770/2015-PSECitation:Zhang Y.-., Xie M., Peng D.-., Zhao J.-., Zhang Z.-. (2016): Dynamics of microbial population size in rhizosphere soil of Monsanto’s Cry1Ac cotton. Plant Soil Environ., 62: 92-97.
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Monsanto’s Bt-cotton NC 33B, planted in northern China for more than one decade, effectively controls cotton bollworms; however the understanding of its potential effects on soil microorganisms is limited. The dynamics of eubacterial, fungal and actinomycetes population sizes in rhizosphere soil of the Bt cotton were analysed by real-time PCR (qPCR) at the different growth stages under field conditions during 2009 to 2011. Results showed that the population sizes (microbial rDNA gene copies) of eubacteria, fungi and actinomycetes in rhizosphere soil were markedly affected by natural variations in the environment related to the year, cotton growth and cultivar. However, there was no significant difference in eubacterial, fungal and actinomycetes population size in rhizosphere soil between the Bt-cotton NC 33B and its near-isogenic comparator DP 5415. In general, the Bt-cotton NC 33B did not show evident effects on the population sizes of eubacteria, fungi and actinomycetes in rhizosphere soil under field conditions after three-year cultivation.

References:
Barriuso Jorge, Valverde José R., Mellado Rafael P., Liles Mark R. (2012): Effect of Cry1Ab Protein on Rhizobacterial Communities of Bt-Maize over a Four-Year Cultivation Period. PLoS ONE, 7, e35481-  https://doi.org/10.1371/journal.pone.0035481
 
Castaldini M., Turrini A., Sbrana C., Benedetti A., Marchionni M., Mocali S., Fabiani A., Landi S., Santomassimo F., Pietrangeli B., Nuti M. P., Miclaus N., Giovannetti M. (): Impact of Bt Corn on Rhizospheric and Soil Eubacterial Communities and on Beneficial Mycorrhizal Symbiosis in Experimental Microcosms. Applied and Environmental Microbiology, 71, 6719-6729  https://doi.org/10.1128/AEM.71.11.6719-6729.2005
 
Donegan K.K., Palm C.J., Fieland V.J., Porteous L.A., Ganio L.M., Schaller D.L., Bucao L.Q., Seidler R.J. (1995): Changes in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endotoxin. Applied Soil Ecology, 2, 111-124  https://doi.org/10.1016/0929-1393(94)00043-7
 
Donegan Katherine K., Schaller Deborah L., Stone Jeffrey K., Ganio Lisa M., Reed Gary, Hamm Philip B., Seidler Ramon J. (1996): Microbial populations, fungal species diversity and plant pathogen levels in field plots of potato plants expressing theBacillus thuringiensis var.tenebrionis endotoxin. Transgenic Research, 5, 25-35  https://doi.org/10.1007/BF01979919
 
Fierer N., Jackson J. A., Vilgalys R., Jackson R. B. (): Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays. Applied and Environmental Microbiology, 71, 4117-4120  https://doi.org/10.1128/AEM.71.7.4117-4120.2005
 
Hannula Silja Emilia, de Boer Wietse, van Veen Johannes, McCluskey Kevin (2012): A 3-Year Study Reveals That Plant Growth Stage, Season and Field Site Affect Soil Fungal Communities while Cultivar and GM-Trait Have Minor Effects. PLoS ONE, 7, e33819-  https://doi.org/10.1371/journal.pone.0033819
 
Icoz I., Saxena D., Andow D. A., Zwahlen C., Stotzky G. (2008): Microbial Populations and Enzyme Activities in Soil In Situ under Transgenic Corn Expressing Cry Proteins from. Journal of Environment Quality, 37, 647-  https://doi.org/10.2134/jeq2007.0352
 
James C. (2014): Global Status of Commercialized Biotech/GM crops: 2014. ISAAA Brief No. 49. Ithaca, International Service for the Acquisition of Agri-biotech Applications.
 
Li Xiaogang, Liu Biao, Cui Jinjie, Liu Doudou, Ding Shuai, Gilna Ben, Luo Junyu, Fang Zhixiang, Cao Wei, Han Zhengmin (2011): No evidence of persistent effects of continuously planted transgenic insect-resistant cotton on soil microorganisms. Plant and Soil, 339, 247-257  https://doi.org/10.1007/s11104-010-0572-2
 
Munger P., Bleiholder H., Hack H., Hess M., Stauß R., Boom T., Weber E. (1998): Phenological Growth Stages of the Cotton Plant (Gossypium hirsutum L.): Codification and Description according to the BBCH Scale. Journal of Agronomy and Crop Science, 180, 143-149  https://doi.org/10.1111/j.1439-037X.1998.tb00384.x
 
Na R.S., Yu H., Yang D.L., Zhao J.N., Li G., Na B.Q., Liu L. (2011): Effect of plantation of transgenic Bt cotton on the amount of rhizospheric soil microorganism and bacterial diversity in the cotton region of Yellow River basin. Chinese Journal of Applied Ecology, 22: 114–120.
 
Pindi P.K., Sultana T. (2013): Bacterial and fungal diversity in rhizosphere soils of Bt and non-Bt cotton in natural systems. Bulgarian Journal of Agricultural Science, 19: 1306–1310.
 
Saxena D, Flores S, Stotzky G (2002): Bt toxin is released in root exudates from 12 transgenic corn hybrids representing three transformation events. Soil Biology and Biochemistry, 34, 133-137  https://doi.org/10.1016/S0038-0717(01)00161-4
 
Saxena D, Stotzky G (2000): Insecticidal toxin from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ. FEMS Microbiology Ecology, 33, 35-39  https://doi.org/10.1111/j.1574-6941.2000.tb00724.x
 
Shen Ren Fang, Cai Hong, Gong Wan He (2006): Transgenic Bt cotton has no apparent effect on enzymatic activities or functional diversity of microbial communities in rhizosphere soil. Plant and Soil, 285, 149-159  https://doi.org/10.1007/s11104-006-9000-z
 
Singh Amit, Singh Major, Dubey Suresh (2013): Changes in Actinomycetes community structure under the influence of Bt transgenic brinjal crop in a tropical agroecosystem. BMC Microbiology, 13, 122-  https://doi.org/10.1186/1471-2180-13-122
 
Singh A.K., Singh M., Dubey S.K. (2014): Rhizospheric fungal community structure of a Bt brinjal and a near isogenic variety. Journal of Applied Microbiology, 117, 750-765  https://doi.org/10.1111/jam.12549
 
Stotzky G. (2005): Persistence and biological activity in soil of the insecticidal proteins from Bacillus thuringiensis, especially from transgenic plants. Plant and Soil, 266, 77-89  https://doi.org/10.1007/s11104-005-5945-6
 
Visser Suzanne, Parkinson Dennis (1992): Soil biological criteria as indicators of soil quality: Soil microorganisms. American Journal of Alternative Agriculture, 7, 33-  https://doi.org/10.1017/S0889189300004434
 
Wei M., Tan F., Zhu H., Cheng K., Wu X., Wang J., Zhao K., Tang X. (2012): Impact of Bt-transgenic rice (SHK601) on soil ecosystems in the rhizosphere during crop development. Plant, Soil and Environment, 58: 217–223.
 
Wu Kongming, Guo Yuyuan, Lv Nan, Greenplate John T., Deaton Randy (2003): Efficacy of Transgenic Cotton Containing a <I>cry1Ac</I> Gene from <I>Bacillus thuringiensis</I> Against <I>Helicoverpa armigera</I> (Lepidoptera: Noctuidae) in Northern China. Journal of Economic Entomology, 96, 1322-1328  https://doi.org/10.1603/0022-0493-96.4.1322
 
Wu Wei Xiang, Liu Wei, Lu Hao Hao, Chen Ying Xu, Devare Medha, Thies Janice (2009): Use of 13C labeling to assess carbon partitioning in transgenic and nontransgenic (parental) rice and their rhizosphere soil microbial communities. FEMS Microbiology Ecology, 67, 93-102  https://doi.org/10.1111/j.1574-6941.2008.00599.x
 
Xiao Yong, Zeng Guang-Ming, Yang Zhao-Hui, Ma Yan-He, Huang Cui, Xu Zheng-Yong, Huang Jing, Fan Chang-Zheng (2011): Changes in the actinomycetal communities during continuous thermophilic composting as revealed by denaturing gradient gel electrophoresis and quantitative PCR. Bioresource Technology, 102, 1383-1388  https://doi.org/10.1016/j.biortech.2010.09.034
 
Zhang Y.J., Xie M., Peng D.L. (2013): Effects of transgenic crops on soil microorganisms: A review. Chinese Journal of Applied Ecology, 24: 2685–2690.
 
Zhang Yan-Jun, Xie Ming, Wu Gang, Peng De-Liang, Yu Wen-Bin (2015): A 3-year field investigation of impacts of Monsanto’s transgenic Bt-cotton NC 33B on rhizosphere microbial communities in northern China. Applied Soil Ecology, 89, 18-24  https://doi.org/10.1016/j.apsoil.2015.01.003
 
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