Comparison of the effect of various long-term fertilization systems on the content and fractional composition of humic compounds in Lessive soilądej W., Żołnowski A.C. (2019): Comparison of the effect of various long-term fertilization systems on the content and fractional composition of humic compounds in Lessive soil. Plant Soil Environ., 65: 172-180.
download PDF

A field experiment was established in 1972 on Luvisol. Three types of fertilizers – cattle manure (CM), cattle slurry (CS) and mineral fertilizers were used. CS was applied in the following doses: I – balanced with CM in terms of the amount of introduced total nitrogen and II – balanced with CM in terms of the amount of introduced total organic carbon (Ctot). 39 years after the experiment was established, half of each experimental plot was limed and since then the experiment was carried in two series – non-limed and limed soils. The paper presents the results of soil analyses 41 years after the experiment was started. It was found that each fertilization system increased the Ctot content in soil in relation to the unfertilized control plot. The increase of Ctot fluctuated between 0.35–6.22 g/kg of dry matter. In both series, the highest Ctot content was observed in the soil fertilized with CM and CM + PK. Limed compared to non-limed soil contained nearly 25% more carbon of humic acids than fulvic acids and nearly 20% lower content of low molecular humic bonds. Liming considerably widened the humic acids carbon:fulvic acids carbon (CHA:CFA) ratio of the fertilized soils, up to 1.32–1.87, while the corresponding objects of the non-limed series showed the CHA:CFA ratio between 0.75–0.97.

Banger K., Kukal S. S., Toor G., Sudhir K., Hanumanthraju T. H. (2009): Impact of long-term additions of chemical fertilizers and farm yard manure on carbon and nitrogen sequestration under rice-cowpea cropping system in semi-arid tropics. Plant and Soil, 318, 27-35
Černý J., Balík J., Kulhánek M., Nedvěd V. (2008): The changes in microbial biomass C and N in long-term field experiments. Plant, Soil and Environment, 54, 212-218
Chen Y., Senesi N., Schnitzer M. (1977): Information Provided on Humic Substances by E4/E6 Ratios1. Soil Science Society of America Journal, 41, 352-
Dębska B. (2004): Properties of humic substances of soil fertilized with slurry. [PhD thesis] Bydgoszcz, Technical and Agricultural Academy. (In Polish)
Diacono Mariangela, Montemurro Francesco (2010): Long-term effects of organic amendments on soil fertility. A review. Agronomy for Sustainable Development, 30, 401-422
Doni S., Macci C., Peruzzi E., Ceccanti B., Masciandaro G. (2014): Factors Controlling Carbon Metabolism and Humification in Different Soil Agroecosystems. The Scientific World Journal, 2014, 1-8
Fließbach Andreas, Oberholzer Hans-Rudolf, Gunst Lucie, Mäder Paul (2007): Soil organic matter and biological soil quality indicators after 21 years of organic and conventional farming. Agriculture, Ecosystems & Environment, 118, 273-284
IUSS, Working Group WRB (2014): World Reference Base for Soil Resources. International Soil Classification System for Naming Soils and Creating Legends for Soil Maps. World Soil Resources Reports No. 106. Rome, FAO. Available at:
Jäger Nadine, Stange Claus Florian, Ludwig Bernard, Flessa Heiner (2011): Emission rates of N2O and CO2 from soils with different organic matter content from three long-term fertilization experiments—a laboratory study. Biology and Fertility of Soils, 47, -
Kawasaki Shigeki, Ikeya Kosuke, Sugiura Yuki, Watanabe Akira (2015): Changes in the composition of humic acids in various upland field soils with a continuous application of an organic amendment as revealed by fractional precipitation analysis. Soil Science and Plant Nutrition, 61, 450-460
Kononova M.M. (1966): Soil Organic Matter. Oxford, Pergamon Press.
Lapa V. V., Seraya T. M., Bogatyreva E. N., Biryukova O. M. (2011): The effect of long-term fertilizer application on the group and fractional composition of humus in a soddy-podzolic light loamy soil. Eurasian Soil Science, 44, 100-104
Lithourgidis Anastasios S., Matsi Theodora, Barbayiannis Nikolaos, Dordas Christos A. (2007): Effect of Liquid Cattle Manure on Corn Yield, Composition, and Soil Properties. Agronomy Journal, 99, 1041-
Moharana P.C., Sharma B.M., Biswas D.R., Dwivedi B.S., Singh R.V. (2012): Long-term effect of nutrient management on soil fertility and soil organic carbon pools under a 6-year-old pearl millet–wheat cropping system in an Inceptisol of subtropical India. Field Crops Research, 136, 32-41
Sądej W., Namiotko A. (2011): Changes in the fractional composition of organic matter in soil caused by long-term organic, mineral and mixed organic and mineral fertilization. Polish Journal of Soil Science, 44: 105–116.
Sądej W., Przekwas K. (2008): Fluctuations of nitrogen levels in soil profile under conditions of a long-term fertilization experiment. Plant, Soil and Environment, 54, 197-203
Sądej Wiera, Żołnowski Andrzej Cezary, Marczuk Olga (2016): Content of phenolic compounds in soils originating from two long-term fertilization experiments. Archives of Environmental Protection, 42, 104-113
Schulz E., Travnikova L.S., Titova N.A., Kogut B.M., Körschens M. (2002): Influence of soil type and fertilization on accumulation and stabilization of organic carbon in different SOM fractions. In: Yuren J. (ed.): Proceedings of 12th ISCO Conference, 3. Technology and Method of Soil and Water Conservation: 304–308. Available at:
Seremesic Srdjan, Ćirić Vladimir, Milošev Dragiša, Vasin Jovica, Djalovic Ivica (2016): Changes in soil carbon stock under the wheat-based cropping systems at Vojvodina province of Serbia. Archives of Agronomy and Soil Science, 63, 388-402
Šimková I., Kuklová M., Kukla J. (2014): Accumulation of Ct and Nt in humus and mineral soil layers: The effect of change of tree species composition in nudal beech forests. Folia Oecologica, 41: 82–91.
Szymańska M., Korc M., Łabętowicz J. (2008): Effects of single liming of sandy soils not limed for more than 40 years in the light of results of long-term fertilizing experiment. Polish Journal of Soil Sciences, 41: 105–114.
Watanabe Akira, Kawasaki Shigeki, Kitamura Shigeyuki, Yoshida Shigekata (2007): Temporal changes in humic acids in cultivated soils with continuous manure application. Soil Science and Plant Nutrition, 53, 535-544
Yan Dezhi, Wang Dejian, Yang Linzhang (2007): Long-term effect of chemical fertilizer, straw, and manure on labile organic matter fractions in a paddy soil. Biology and Fertility of Soils, 44, 93-101
Yanardağ İbrahim Halil, Zornoza R., Cano A. Faz, Yanardağ A. Büyükkılıç, Mermut A. R. (2015): Evaluation of carbon and nitrogen dynamics in different soil types amended with pig slurry, pig manure and its biochar by chemical and thermogravimetric analysis. Biology and Fertility of Soils, 51, 183-196
download PDF

© 2019 Czech Academy of Agricultural Sciences