Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L
J.I. Flores Solís, P. Mlejnek, K. Studená, S. Procházkahttps://doi.org/10.17221/4122-PSECitation:Flores Solís J.I., Mlejnek P., Studená K., Procházka S. (2003): Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L. Plant Soil Environ., 49: 255-260.
Chenopodium rubrum belongs to the plant species in which standard Agrobacterium-mediated transformation procedures remain inefficient. We demonstrate that the employment of sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively enhanced transient expression of GUS gene coding for b-glucuronidase in Chenopodium rubrum. Further the results indicated that the age of seedlings is one of the limiting factors affecting the potency of Agrobacterium tumefaciens infection. Histochemical detection of b-glucuronidase activity revealed that two-days-old seedlings were much more susceptible to infection than ten-days-old ones. According to our results SAAT technology could provide an efficient tool for obtaining stable transformants when applied to two-days-old seedlings of Chenopodium rubrum.Keywords:
Agrobacterium tumefaciens; strain EHA 105; uidA; GUS; b-glucuronidase