Using DNA markers for characterisation of tomato resistance against root nematoden Meloidogyne incognita
S. Skupinová, P. Vejl, P. Sedlák, M. Bardová, L. Srbek, P. Klápště, M. Zouhar, B. Tesařováhttps://doi.org/10.17221/3681-PSECitation:Skupinová S., Vejl P., Sedlák P., Bardová M., Srbek L., Klápště P., Zouhar M., Tesařová B. (2004): Using DNA markers for characterisation of tomato resistance against root nematoden Meloidogyne incognita. Plant Soil Environ., 50: 59-64.
CAPS (Cleaved Amplified Polymorphic Sequence) method and standard infection tests were used for the study of tomatoes (Lycopersicon esculentum L.) resistance against the root-knot nematode (Meloidogyne incognita Kofoit and White). The CAPS method was used to determine genotypic constitution of Mi gene in ten Czech and four foreign varieties of tomatoes. Similarly were verified one hundred and twenty individuals of F2 segregated progeny of Nema variety and the same number of F2 progeny of Petopride variety. A comparison of results of infection test with results of molecular-genetic analyse of DNA showed 100% congruence in detection resistant (susceptible respectively) genotypes. Result of 2 test confirmed in the level of mathematical expectation P (0.5, 0.7) congruence between actually obtained and theoretical segregation ratios. The CAPS method can be recommended for fast and precise determination of resistance or susceptibility of tomato plants against the root-knot nematodes. The goal of the method is the possibility of detection of genotypic constitution of Mi gene that can significantly accelerate the process of creation of new resistant varieties.Keywords:
tomato; Lycopersicon esculentum L.; Meloidogyne incognita Kofoit and White; resistance; CAPS; DNA; marker; PCR