Alleles controlling apple skin colour and incompatibility in new Czech apple varieties with different degrees of resistance against Venturia inaequalis CKE.

https://doi.org/10.17221/3557-PSECitation:Melounová M., Vejl P., Sedlák P., Blažek J., Zoufalá J., Milec Z., Blažková H. (2005): Alleles controlling apple skin colour and incompatibility in new Czech apple varieties with different degrees of resistance against Venturia inaequalis CKE. Plant Soil Environ., 51: 65-73.
download PDF
The skin colour of 21 varieties was assessed on a molecular level. Four varieties had yellow skin colour with the genotype constitution a1a1, a1a2 or a2a2. Seventeen varieties had dominant red colour. Homozygous A1A1 constitution was present in 7 varieties, and heterozygous constitution A1a1 or A1a2 was found in the rest of the assessed varieties. The S-alleles controlled the incompatibility system of the pistil. Their detection is possible by means of the PCR method on the basis of allele specific primers. Six S-alleles (S1, S2, S3, S5, S7 and S9) were studied in the same collection of 21 diploid Czech varieties. This paper brings new findings on S-allele characterization, because the Czech varieties have not yet been studied on a molecular level. Both types of S-allele were found in 12 varieties. Only one type of S-allele was described in 9 varieties. Simultaneously, the presence of the Vf gene was screened in the collection of 21 Czech apple varieties. Ten varieties with a field resistance against the scab had a heterozygous constitution of the Vf gene. All 11 susceptible varieties were recessive homozygous vfvf.
download PDF

© 2020 Czech Academy of Agricultural Sciences