Mapping of non-recombining regions via molecular markers
B. Janoušek, J. Žlůvováhttps://doi.org/10.17221/2207-PSECitation:Janoušek B., Žlůvová J. (2007): Mapping of non-recombining regions via molecular markers. Plant Soil Environ., 53: 321-324.
The lack of recombination in some genomic regions represents a serious obstacle in mapping studies. In this review, we describe methods that are currently used to overcome this problem. Main attention is given to the comparison of methods that are based on the principle of radiation hybrid mapping. We also discuss a strategy called HAPPY mapping (mapping based on the analysis of approximately HAPloid DNA samples using the PolYmerase chain reaction). In addition to reviewing the methods used by other authors, we also summarize our experience with deletion mapping of Y-chromosome in dioecious model plant species (Silene latifolia).Keywords:physical maps; radiation hybrid mapping; recombination; deletion; HAPPY mapping; RH mapping software