Cot-based cloning and sequencing of the short arm of wheat chromosome 1B
H. Šimková, J. Janda, E. Hřibová, J. Šafář, J. Doleželhttps://doi.org/10.17221/2195-PSECitation:Šimková H., Janda J., Hřibová E., Šafář J., Doležel J. (2007): Cot-based cloning and sequencing of the short arm of wheat chromosome 1B. Plant Soil Environ., 53: 437-441.
Sequencing of cereal genomes is not a feasible task due to their large size and high content of repetitive DNA sequences. There are two basic approaches to simplify analysis of such genomes: reduced representation approaches, such as EST sequencing, methyl filtration and Cot-based cloning and sequencing; on the other side there is analysis of genomes in a step-wise manner, e.g. through creation of chromosome-specific genomic resources. Combination of both approaches – i.e. Cot-based cloning and sequencing of DNA obtained from a chromosome-arm-specific BAC library – was tested in this work.Keywords:Cot; CBCS; Cot filtration; BAC library; 1 BS; wheat; genome analysis