The extremely low frequency electromagnetic field (ELF-EMF) occurs naturally from the earth and artificially as a human invention. The objectives of this study were to develop a suitable ELF-EMF generator for in vitro plants culture studies and to determine the effect of ELF-EMF exposure on in vitro tobacco (Nicotiana tabacum) growth and chlorophyll content. An ELF-EMF generator, the coGEM 1,000 was constructed using four coils of copper wires that were connected to a transformer, multimeter and rheostat. The coGEM 1,000 suitable for tissue culture plants is able to produce stable and uniform 6 and 12 mT 50Hz ELF-EMF in the four coils of the ELF-EMF generator. The tobacco in vitro plantlets were exposed to 6 and 12 mT of 50 Hz ELF-EMF for a period of 0.5, 1, 2 and 4 hours. The exposure to 12 mT ELF-EMF for an hour increased plant growth (shoot height); whereas the exposure to 6 mT Elf-EMF for an hour increased chlorophyll a, chlorophyll b and the total chlorophyll content.
Afreen F. (2005): Physiological and anatomical characteristics of in vitro photoautotrophic plants. In: Kozai T. (ed.): Photoautotrophic (Sugar-FRee Medium) Micropropagation as a New Propagation and Transplant Production System. The Netherlands, Springer: 61–90.
Aladjadjiyan A., Ylieva T. (2003): Influence of stationary magnetic field on the early stages of the development of the tobacco seeds (Nicotiana tabacum L.). Journal of Central European Agriculture, 4: 131–138.
Budzianowska A. ( 2009): In vitro cultures of tobacco and their impact on development of plant biotechnology. Przeglad Lekarski, 66: 890–893.
Calestino C., Picazo M.L., Toribio M., Alvarez-Ude J.A., Bardasano J.L (1998): Influence of 50 Hz electromagnetic fields on recurrent embryogenesis and germination of cork oak somatic embryo. Plant Cell, Tissue and Organ Culture, 54: 65–69. https://doi.org/10.1023/A:1006034510170
Chrysikopoulos H.S. (2009): Clinical MR imaging and physics. Berlin, Heidelberg, Springer.
Damaraju Sridevi, Schlede Stephanie, Eckhardt Ulrich, Lokstein Heiko, Grimm Bernhard (2011): Functions of the water soluble chlorophyll-binding protein in plants. Journal of Plant Physiology, 168, 1444-1451 https://doi.org/10.1016/j.jplph.2011.02.007
Dardeniz A., Tayyar S., Yalcin S. (2006): Influence of low frequency electromagnetic field on the vegetative growth grape CV. Uslu. Journal of Central European Agriculture, 7: 389–396.
Ganapathi T.R., Suprasanna P., Rao P.S., Bapat V.A. (2004): Tobacco (Nicotiana tabacum L.) – A modeling system for tissue culture interventions and genetic engineering. Indian Journal of Biotechnology, 3: 171–184.
Genkov T., Tsoneva P., Ivanova I. (1997): Effect of cytokinins on photosynthetic pigments and chlorophyllase activity in in vitro cultures of axillary buds of Dianthus caryophyllus L.
Journal of Plant Growth Regulator, 16: 169–172.
Gitelson Anatoly A., Gritz † Yuri, Merzlyak Mark N. (2003): Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves. Journal of Plant Physiology, 160, 271-282 https://doi.org/10.1078/0176-1617-00887
Goldsworthy A. (2006): Effects of electrical and electromagnetic fields on plants and related topics. In: Goldsworthy A. (ed.): Plant Electrophysiology: Theory and Methods. Volkov, Springer, Verlag: 247–267.
Huang H.H., Wang S.R. (2007): The effects of 60Hz magnetic fields on plant growth. Nature and Science, 5: 59–68.
Kadleček Petr, Rank Barbara, Tichá Ingrid (2003): Photosynthesis and photoprotection inNicotiana tabacumL.in vitro-grown plantlets. Journal of Plant Physiology, 160, 1017-1024 https://doi.org/10.1078/0176-1617-00980
Mihai Radu, Cogalniceanu Gina, Brezeanu Aurelia (2009): Control of Nicotiana Tabacum Callus Growth by Alternating and Pulsed Electric Field. Electro- and Magnetobiology, 13, 195-201 https://doi.org/10.3109/15368379409030716
Ben-Izhak Monselise Edna, Parola Abraham H, Kost Daniel (2003): Low-frequency electromagnetic fields induce a stress effect upon higher plants, as evident by the universal stress signal, alanine. Biochemical and Biophysical Research Communications, 302, 427-434 https://doi.org/10.1016/S0006-291X(03)00194-3
Mrozynski G., Stallein M. (2013): Electromagnetic field theory. Weosbaden, Vieweg+Teubner Verlag.
Murashige Toshio, Skoog Folke (1962): A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Ni Z., Kim E.D., Ha M., Lackey E., Liu J., Zhang Y., Sun Q., Chen Z.J. (2009): Altered circadian rhythms regulate growth vigor in hybrids and allopolyploids. Nature Protocols, 457: 327–332.
Nimmi V., Madhu G. (2009): Effect of pre-sowing treatment with permanent magnetic field on germination and growth of chilli (Capsicum annum L.). International Agrophysics, 23: 195–198.
Odhiambo O.J., Francis N.G., Isabel W.N. (2009): The influence of electromagnetic fields on the initial growth rate of Phaseolus vulgaris . Journal of Applied Biosciences, 22: 1350–1358.
Oláh Roman, Masarovičová Elena (1998): Photosynthesis, respiration, and chlorophylls in presenescent, regreened, and senescent leaves of forest herb Smyrnium perfoliatum L. (Apiaceae). Acta Physiologiae Plantarum, 20, 173-178 https://doi.org/10.1007/s11738-998-0010-8
Pazur A., Rassadina V. (2009): Transient effect of weak electromagnetic fields on calcium ion concentration in Arabidopsis thaliana. Available at http://www.biomedcentral.com/1471-2229/9/4
Piacentini Maria Piera, Fraternale Daniele, Piatti Elena, Ricci Donata, Vetrano Flavio, Dachà Marina, Accorsi Augusto (2001): Senescence delay and change of antioxidant enzyme levels in Cucumis sativus L. etiolated seedlings by ELF magnetic fields. Plant Science, 161, 45-53 https://doi.org/10.1016/S0168-9452(01)00380-6
Pietruszewski P., Muszynski S., Dziwulska A. (2007): Electromagnetic fields and electromagnetic radiation as non-invasive external stimulations for seeds (selected methods and responses). International Agrophysics, 21: 95–100.
Reed David D., Jones Elizabeth A., Mroz Glenn D., Liechty Hal O., Cattelino Peter J., J�rgensen Martin F. (1993): Effects of 76 Hz electromagnetic fields on forest ecosystems in northern Michigan: Tree growth. International Journal of Biometeorology, 37, 229-234 https://doi.org/10.1007/BF01387529
Rudiger W. (2009): Regulation of the late steps of chlorophylls biosynthesis. In: Waren, M.J., A.G. Smith (eds.): Tetrapyrroles: Birth, life, and death. Landes Bioscience and Springer Science+Bussiness Media: 263–273.
Shabrangi A., Majd A., Sheidai M. (2013): Effect of extremely low frequency electromagnetic field on growth, cytogenetic, protein content, and antioxidant enzymes of Zea Mays L. African Journal of Biotechnology, 10: 9362–9369.
Tanaka Ryouichi, Tanaka Ayumi (2011): Chlorophyll cycle regulates the construction and destruction of the light-harvesting complexes. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1807, 968-976 https://doi.org/10.1016/j.bbabio.2011.01.002
Dao-liang Yan, Yu-qi Guo, Xue-ming Zai, Shu-wen Wan, Qin Pei (2009): Effects of electromagnetic fields exposure on rapid micropropagation of beach plum (Prunus maritima). Ecological Engineering, 35, 597-601 https://doi.org/10.1016/j.ecoleng.2008.04.017