Already a short-term soils exposure to the field-rate glufosinate concentration significantly influences soil bacterial communitiesčáková A., Legáth J., Pristaš P., Javorský P. (2015): Already a short-term soils exposure to the field-rate glufosinate concentration significantly influences soil bacterial communities. Soil & Water Res., 10: 271-277.
download PDF
The early impact of glufosinate derived herbicide Basta® 15 on bacterial communities of two different soils never exposed to this herbicide was investigated using cultivation approach and non-cultivation based denaturing gradient gel electrophoresis (DGGE) analysis of amplified 16S rRNA genes. Under the simulated laboratory conditions glufosinate treatment increased numbers of total cultivable heterotrophic bacteria in both tested soils. Surprisingly even the lowest glufosinate concentration (1 mmol) significantly affected bacterial community composition in both tested soils and original populations were replaced by new ones upon the 2 days glufosinate treatment. In nutrient rich Haniska soil the effect was dose dependent and glufosinate treatment decreased genetic diversity of bacterial population. In nutrient poor Kaľava soil the highest glufosinate concentration (16 mmol) increased the diversity of bacterial population probably as a result of carbon source supplementation. Glufosinate treatment selected Gram-negative bacteria in both soils. Two species of Enterobacter genus were found to be dominant in glufosinate treated Haniska soil and Pseudomonas beteli and Brevundimonas diminuta were found to be dominant in glufosinate treated Kaľava soil using non-cultivation based DGGE method. Our data indicated that under the simulated soil conditions the soil bacterial community was significantly affected even by a short-term exposure to glufosinate.
Ahmad I., Malloch D. (1995): Interaction of soil microflora with the bioherbicide phosphinothricin. Agriculture, Ecosystems & Environment, 54: 165–174.
Bartsch K., Tebbe C.C. (1989): Initial steps in degradation of phosphinothricin (glufosinate) by soil bacteria. Applied and Environmental Microbiology, 55: 711–716.
Chen W.C, Yen J.H., Chang C.S., Wang Y.S. (2008): Effects of herbicide butachlor on soil microorganisms and on nitrogen-fixing abilities in paddy soil. Ecotoxicology and Environmental Safety, 72: 120–27.
Colanduoni John A., Villafranca Joseph J. (1986): Inhibition of Escherichia coli glutamine synthetase by phosphinothricin. Bioorganic Chemistry, 14, 163-169
el Fantroussi S., Verschuere L., Verstraete W., Top E.M. (1999): Effect of phenylurea herbicides on soil microbial communities estimated by analysis of 16S rRNA gene fingerprints and community-level physiological profiles. Applied and Environmental Microbiology, 65: 982–988.
Ernst Dieter, Rosenbrock-Krestel Hilkea, Kirchhof Gudrun, Bieber Evi, Giunaschwili Nathela, Müller Rüdiger, Fischbeck Gerhard, Wagner Tobias, Sandermann Heinrich, Hartmann Anton (2008): Molecular Investigations of the Soil, Rhizosphere and Transgenic Glufosinate-Resistant Rape and Maize Plants in Combination with Herbicide (Basta®) Application under Field Conditions. Zeitschrift für Naturforschung C, 63, -
Gallina Marco A., Stephenson Gerald R. (1992): Dissipation of [14C]glufosinate ammonium in two Ontario soils. Journal of Agricultural and Food Chemistry, 40, 165-168
Griffiths B. S., Caul S., Thompson J., Hackett C. A., Cortet J., Pernin C., Krogh P. H. (2008): Soil microbial and faunal responses to herbicide tolerant maize and herbicide in two soils. Plant and Soil, 308, 93-103
Hsiao Chau-Ling, Young Chiu-Chung, Wang Ching-Yuh (2007): Screening and Identification of Glufosinate-Degrading Bacteria from Glufosinate-Treated Soils. Weed Science, 55, 631-637
Jensen Sigmund, ØVreÃ¥s Lise, Daae Frida Lise, Torsvik Vigdis (1998): Diversity in methane enrichments from agricultural soil revealed by DGGE separation of PCR amplified 16S rDNA fragments. FEMS Microbiology Ecology, 26, 17-26
Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H., Yi H., Won S., Chun J. (): Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 62, 716-721
Kriete G., Broer I. (1996): Influence of the herbicide phosphinothricin on growth and nodulation capacity of Rhizobium meliloti. Applied Microbiology and Biotechnology, 46, 580-586
Lerner A., Shor Y., Vinokurov A., Okon Y., Jurkevitch E. (2006): Can denaturing gradient gel elektrophoresis (DGGE) analysis of amplified 16S rDNA of soil bacterial populations be used in forensic invenstigations? Soil Biology and Biochemistry, 38: 1188–1192.
L�pez-Siles F. J., C�rdenas J., Franco A. R. (1999): Biochemical and genetic analysis of a Chlamydomonas reinhardtii mutant devoid of chloroplastic glutamine synthetase activity. Planta, 207, 436-441
Luo H.F., Qi H.Y., Zhang H.X. (2004): Diversity surveys of soil bacterial community by cultivation-based methods and molecular fingerprinting techniques. Journal of Environmental Science (China), 16: 581–584.
Morel Melanie, Buee Marc, Chalot Michel, Brun Annick (2006): NADP-dependent glutamate dehydrogenase: a dispensable function in ectomycorrhizal fungi. New Phytologist, 169, 179-190
Mueller J.G., Skipper H.D., Lawrence E.G., Kline E.L. (1989): Bacterial stimulation by carbamothioate herbicides. Weed Science, 37: 424–427.
Murray A.E., Hollibaugh J.T., Orrego C. (1996): Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments. Applied and Environmental Microbiology, 62: 2676–2680.
Nakatsu Cindy H., Torsvik Vigdis, Øvreås Lise (2000): Soil Community Analysis Using DGGE of 16S rDNA Polymerase Chain Reaction Products. Soil Science Society of America Journal, 64, 1382-
Nübel U., Engelen B., Felske A., Snaidr J., Wieshuber A., Amann R.I., Ludwig W., Backhaus H. (1996): Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. Journal of Bacteriology, 178: 5636–5643.
Nur Masirah M.Z., Mohamad R.B., Kamaruzaman S., Morshed M.M., Awang Y.H. (2013): Growth-inhibitory effects of herbicides on soil bacterial population in oil palm plantation. Journal of Pure and Applied Microbiology, 7: 1799–1808.
Rappé Michael S., Giovannoni Stephen J. (2003): The Uncultured Microbial Majority. Annual Review of Microbiology, 57, 369-394
Seghers Dave, Verthé Kristof, Reheul Dirk, Bulcke Robert, Siciliano Steven D, Verstraete Willy, Top Eva M (2003): Effect of long-term herbicide applications on the bacterial community structure and function in an agricultural soil. FEMS Microbiology Ecology, 46, 139-146
Smith Allan E., Belyk Murray B. (1989): Field Persistence Studies with the Herbicide Glufosinate-Ammonium in Saskatchewan Soils. Journal of Environment Quality, 18, 475-
Tebbe Christoph C., Reber Hans H. (1988): Utilization of the herbicide phosphinothricin as a nitrogen source by soil bacteria. Applied Microbiology and Biotechnology, 29, 103-105
Torsvik V., Goksoyr J., Daae F.L. (1990): High diversity in DNA of soil bacteria. Applied and Environmental Microbiology, 56: 782–787.
Tothova Timea, Sobekova Anna, Holovska Katarina, Legath Jaroslav, Pristas Peter, Javorsky Peter (2010): Natural glufosinate resistance of soil microorganisms and GMO safety. Open Life Sciences, 5, -
Vallaeys Tatiana, Topp Edward, Muyzer Gerard, Macheret Valérie, Laguerre Gisèle, Rigaud Annabel, Soulas Guy (1997): Evaluation of denaturing gradient gel electrophoresis in the detection of 16S rDNA sequence variation in rhizobia and methanotrophs. FEMS Microbiology Ecology, 24, 279-285
Varadyova Z., Mihalikova K., Kisidayova S., Javorsky P. (2006): Fermentation pattern of the rumen and hindgut inocula of sheep grazing on the area polluted from non-ferrous metal industry. Czech Journal of Animal Science, 51: 66–72.
Quinn J.P., Heron J.K., McMullan G. (1993): Glufosinate tolerance and utilization by soil and aquatic bacteria. Biology and Environment Proceedings of the Royal Irish Academy, 93: 181–186.
Ward David M., Weller Roland, Bateson Mary M. (1990): 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature, 345, 63-65
Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. (1991): 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173: 697–703.
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti