Relationships between soil physicochemical properties and nitrogen fixing, nitrifying and denitrifying under varying land-use practices in the northwest region of Argentinaérez Brandan C., Meyer A., Meriles J.M., Huidobro J., Schloter M., Vargas Gil S. (2019): Relationships between soil physicochemical properties and nitrogen fixing, nitrifying and denitrifying under varying land-use practices in the northwest region of Argentina. Soil & Water Res., 14: 1-9.
download PDF

The aim of this study was to evaluate the response pattern of diazotrophic microbes, denitrifiers and nitrifiers to different types of land use management, such as soybean monoculture (M) during 5 and 24 years (M5 and M24) and soybean-maize rotation (R) during 4 and 15 years (R4 and R15) in two subsequent years at the time point of flowering. Soil samples from a site recently introduced into agriculture (RUA) and a pristine soil under native vegetation (NV) were used as controls. Abundances of different functional groups of microbes were assessed using the direct quantification of marker genes by quantitative real-time PCR using extracted DNA from rhizosphere samples. In addition, soil chemical and physical properties were analysed and correlated with the abundance data from the functional microbial groups under investigation. Overall, the results indicate that the abundance of nifH genes was higher under R treatments compared to M treatments. The abundance of ammonium monooxygenase genes amoA (AOA) was generally higher under rotation systems and decreased under M24. RUA evidenced a negative effect on the establishment and development of AOA communities. The influence of land use on nirS abundance was inconsistent. However, R treatments showed a high abundance of nirK genes compared to M treatments. In both growing seasons, the abundance of nosZ genes was higher under NV compared with the other treatments. Furthermore, M24 treatment was related to strongly changed chemical and physical soil properties compared with the other sites. As expected, soil samples from RUA showed the strong dynamics of measured parameters indicating the high sensitivity of soils under transition to environmental parameters. Our results also indicated that the long-term crop rotation modified the abundance of the investigated microbial groups compared to the monoculture and increased soil chemical and physical quality. Therefore, our results provide evidence for a stimulatory effect of the long-term crop rotation on the abundance of microbes involved in N transformation.

Barrena Iskander, Menéndez Sergio, Correa-Galeote David, Vega-Mas Izargi, Bedmar Eulogio J., González-Murua Carmen, Estavillo José M. (2017): Soil water content modulates the effect of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on nitrifying and denitrifying bacteria. Geoderma, 303, 1-8
Bárta Jiří, Melichová Tereza, Vaněk Daniel, Picek Tomáš, Šantrůčková Hana (2010): Effect of pH and dissolved organic matter on the abundance of nirK and nirS denitrifiers in spruce forest soil. Biogeochemistry, 101, 123-132
Black C., Allison L. (1965): Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties. Agronomy Monograph No. 9, Wisconsin, American Society of Agronomy: 1367–1378.
Blake G.R., Hartge K.H. (1986): Bulk Density. Madison, Soil Science Society of America Press.
Bremner J.M. (1996): Nitrogen Total. Madison, Soil Science Society of America Press.
Castrillo A., Schulz G., Rodríguez D. (2014): Soil Map of Argentina, Las Lajitas, Anta Department. Salta Province. INTA Editions.
Ciccolini Valentina, Bonari Enrico, Ercoli Laura, Pellegrino Elisa (2016): Phylogenetic and multivariate analyses to determine the effect of agricultural land-use intensification and soil physico-chemical properties on N-cycling microbial communities in drained Mediterranean peaty soils. Biology and Fertility of Soils, 52, 811-824
Corvalán E., Franzoni A., Huidobro J., Arzeno J. (2000): Microsieves method to estimate soil aggregate stability 1−2 mm. In: 14th Soil Science Congress, Mar del Plata. Commission I − Panel 25.
Di Rienzo J.A., Casanoves F., Balzarini M.G., González L., Tablada M., Robledo C.W. (2016): Infostat Group. Cordoba, FCA, UNC.
Hai B., Diallo N. H., Sall S., Haesler F., Schauss K., Bonzi M., Assigbetse K., Chotte J.-L., Munch J. C., Schloter M. (2009): Quantification of Key Genes Steering the Microbial Nitrogen Cycle in the Rhizosphere of Sorghum Cultivars in Tropical Agroecosystems. Applied and Environmental Microbiology, 75, 4993-5000
Hatzenpichler R., Lebedeva E. V., Spieck E., Stoecker K., Richter A., Daims H., Wagner M. (2008): A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring. Proceedings of the National Academy of Sciences, 105, 2134-2139
Henry S., Bru D., Stres B., Hallet S., Philippot L. (2006): Quantitative Detection of the nosZ Gene, Encoding Nitrous Oxide Reductase, and Comparison of the Abundances of 16S rRNA, narG, nirK, and nosZ Genes in Soils. Applied and Environmental Microbiology, 72, 5181-5189
Burger Martin, Jackson Louise E (2003): Microbial immobilization of ammonium and nitrate in relation to ammonification and nitrification rates in organic and conventional cropping systems. Soil Biology and Biochemistry, 35, 29-36
Kowalchuk George A., Stienstra Atie W., Heilig G.Hans J., Stephen John R., Woldendorp Jan W. (2000): Molecular analysis of ammonia-oxidising bacteria in soil of successional grasslands of the Drentsche A (The Netherlands). FEMS Microbiology Ecology, 31, 207-215
Lauber Christian L., Strickland Michael S., Bradford Mark A., Fierer Noah (2008): The influence of soil properties on the structure of bacterial and fungal communities across land-use types. Soil Biology and Biochemistry, 40, 2407-2415
Li Lingling, Larney Francis J., Angers Denis A., Pearson Drusilla C., Blackshaw Robert E. (2015): Surface Soil Quality Attributes following 12 Years of Conventional and Conservation Management on Irrigated Rotations in Southern Alberta. Soil Science Society of America Journal, 79, 930-
Maharjan Menuka, Sanaullah Muhammad, Razavi Bahar S., Kuzyakov Yakov (2017): Effect of land use and management practices on microbial biomass and enzyme activities in subtropical top-and sub-soils. Applied Soil Ecology, 113, 22-28
Martir-Torres Maina C., Bruns Mary Ann (2013): Comparative diversity and abundance of ammonia monooxygenase genes in mulched and vegetated soils. Soil Biology and Biochemistry, 57, 758-768
Prosser James I. (2012): Ecosystem processes and interactions in a morass of diversity. FEMS Microbiology Ecology, 81, 507-519
Rabbi A.T.M.Z., Cameron D.A., Rahman M.M. (2014): Effect of initial partial saturation on collapse behavior of glacial sand with fines. In: Geo-Congress 2014. Technical Papers. ASCE Library. Available at
Schloter M., Dilly O., Munch J.C. (2003): Indicators for evaluating soil quality. Agriculture Ecosystem & Environment, 98: 255–262.
Stone Madeleine M., Kan Jinjun, Plante Alain F. (2015): Parent material and vegetation influence bacterial community structure and nitrogen functional genes along deep tropical soil profiles at the Luquillo Critical Zone Observatory. Soil Biology and Biochemistry, 80, 273-282
Wang Ying, Marschner Petra, Zhang Fusuo (2012): Phosphorus pools and other soil properties in the rhizosphere of wheat and legumes growing in three soils in monoculture or as a mixture of wheat and legume. Plant and Soil, 354, 283-298
Zumft W.G. (1997): Cell biology and molecular basis of denitrification. Microbiology Molecular Biology Review, 61: 533–616.
download PDF

© 2019 Czech Academy of Agricultural Sciences