Glomalin – an interesting protein part of the soil organic matterček V., Pohanka M. (2020): Glomalin – an interesting protein part of the soil organic matter. Soil & Water Res., 15: 67-74.
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The negative effects of the current agricultural practices include erosion, acidification, loss of soil organic matter (dehumification), loss of soil structure, soil contamination by risky elements, reduction of biological diversity and land use for non-agricultural purposes. All these effects are a huge risk to the further development of soil quality from an agronomic point of view and its resilience to projected climate change. Organic matter has a crucial role in it. Relatively significant correlations with the quality or the health of soil parameters and the soil organic matter or some fraction of the soil organic matter have been found. In particular, Ctot, Cox, humic and fulvic acids, the C/N ratio, and glomalin. Our work was focused on glomalin, a glycoprotein produced by the hyphae and spores of arbuscular mycorrhizal fungi (AMF), which we classify as Glomeromycota. Arbuscular mycorrhiza, and its molecular pathways, is not a well understood phenomenon. It appears that many proteins are involved in the arbuscular mycorrhiza from which glomalin is probably one of the most significant. This protein is also responsible for the unique chemical and physical properties of soils and has an ecological and economical relevance in this sense and it is a real product of the mycorrhiza. Glomalin is very resistant to destruction (recalcitrant) and difficult to dissolve in water. Its extraction requires specific conditions: high temperature (121°C) and a citrate buffer with a neutral or alkaline pH. Due to these properties, glomalin (or its fractions) are very stable compounds that protect the soil aggregate surface. In this review, the actual literature has been researched and the importance of glomalin is discussed.


Augé R.M. (2004): Arbuscular mycorrhizae and soil/plant water relations. Canadian Journal of Soil Science, 84: 373–381.
Batten K.M., Six J., Scow K.M., Rillig M.C. (2005): Plant invasion of native grassland on serpentine soils has no major effects upon selected physical and biological properties. Soil Biology and Biochemistry, 37: 2277–2282.
Blackwell M. (2000): Terrestrial life – Fungal from the start? Science, 289: 1884–1885.
Błaszkowski J. (2012): Glomeromycota. Kraków, W. Szafer Institute of Botany, Polish Academy of Sciences.
Borowicz V.A. (2001): Do arbuscular mycorrhizal fungi alter plant–pathogen relations? Ecology, 82: 3057–3068.[3057:DAMFAP]2.0.CO;2
Cheng Y.F., Sun J.R., Chen H.B., Adam A., Tang S., Kemper N., Hartung J., Bao E.D. (2016): Expression and location of HSP60 and HSP10 in the heart tissue of heat-stressed rats. Experimental and Therapeutic Medicine, 12: 2759–2765.
Chern E.C., Tsai D.W., Ogunseitan O.A. (2007): Deposition of glomalin-related soil protein and sequestered toxic metals into watersheds. Environmental Science & Technology, 41: 3566–3572.
Cornejo P., Meier S., Borie G., Rillig M.C., Borie F. (2008): Glomalin-related soil protein in a Mediterranean ecosystem affected by a copper smelter and its contribution to Cu and Zn sequestration. Science of the Total Environment, 406: 154–160.
Curaqueo G., Barea J.M., Acevedo E., Rubio R., Cornejo P., Borie F. (2011): Effects of different tillage system on arbuscular mycorrhizal fungal propagules and physical properties in a Mediterranean agroecosystem in central Chile. Soil and Tillage Research, 113: 11–18.
Driver J.D., Holben W.E., Rillig M.C. (2005): Characterization of glomalin as a hyphal wall component of arbuscular mycorrhizal fungi. Soil Biology and Biochemistry, 37: 101–106.
Fierer N., Schimel J.P., Cates R.G., Zou J. (2001): Influence of balsam poplar tannin fractions on carbon and nitrogen dynamics in Alaskan taiga floodplain soils. Soil Biology and Biochemistry, 33: 1827–1839.
Gadkar V., Rillig M.C. (2006): The arbuscular mycorrhizal fungal protein glomalin is a putative homolog of heat shock protein 60. FEMS Microbiology Letters, 263: 93–101.
Gałązka A., Gawryjołek K. (2015): Glomalin – soil glicoprotein produced by arbuscular mycorhizal fungus. Advancements of Microbiology, 54: 331–343. (in Polish)
Gałązka A., Gawryjołek K., Grządziel J., Księżak J. (2017): Effect of different agricultural management practices on soil biological parameters including glomalin fraction. Plant, Soil and Environment, 63: 300–306.
Gałązka A., Gawryjołek K., Gajda A., Furtak K., Księżniak A., Jończyk K. (2018): Assessment of the glomalins content in the soil under winter wheat in different crop production systems. Plant, Soil and Environment, 64: 32–37.
Gammazza A.M., Bucchieri F., Grimaldi L.M.E., Benigno A., de Macario E.C., Macario A.J.L., Zummo G., Cappello F. (2012): The molecular anatomy of human hsp60 and its similarity with that of bacterial orthologs and acetylcholine receptor reveal a potential pathogenetic role of anti-chaperonin immunity in myasthenia gravis. Cellular and Molecular Neurobiology, 32: 943–947.
Gao Y., Zhou Z., Ling W., Hu X., Chen S. (2017): Glomalin-related soil protein enhances the availability of polycyclic aromatic hydrocarbons in soil. Soil Biology and Biochemistry, 107: 129–132.
García-Orenes F., Roldán A., Mataix-Solera J., Cerdà A., Campoy M., Arcenegui V., Caravaca F. (2012): Soil structural stability and erosion rates influenced by agricultural management practices in a semi-arid Mediterranean agro-ecosystem. Soil Use and Management, 28: 571–579.
Gillespie A.W., Farrell R.E., Walley F.L., Ross A.R.S., Leinweber P., Eckhardt K.U., Regier T.Z., Blyth R.I.R. (2011): Glomalin-related soil protein contains non-mycorrhizal-related heat-stable proteins, lipids and humic materials. Soil Biology and Biochemistry, 43: 766–777.
Gomez-Bellot M.J., Nortes P.A., Ortuno M.F., Romero-Trigueros C., Fernandez-Garcia N., Sanchez-Blanco M.J. (2015): Influence of arbuscular mycorrhizal fungi and treated wastewater on water relations and leaf structure alterations of Viburnum tinus L. plants during both saline and recovery periods. Journal of Plant Physiology, 188: 96–105.
Harley J.L., Smith S.E. (1983): Mycorrhizal Symbiosis. London, Academic Press.
Harner M.J., Ramsey P.W., Rillig M.C. (2004): Protein accumulation and distribution in floodplain soils and river foam. Ecology Letters, 7: 829–836.
Harper C.J., Taylor T.N., Krings M., Taylor E.L. (2013): Mycorrhizal symbiosis in the Paleozoic seed fern Glossopteris from Antarctica. Review of Palaeobotany and Palynology, 192: 22–31.
Harris K.K., Paul E.A. (1987): Carbon requirements of vesicular arbuscular mycorrhizae. In: Safir G.R. (ed.): Ecophysiology of VA Mycorrhizal Plants. Boca Raton, CRC Press: 93–105.
Hättenschwiler S., Vitousek P.M. (2000): The role of polyphenols in terrestrial ecosystem nutrient cycling. Trends in Ecology and Evolution, 15: 238–243.
Helgason T., Fitter A.H., Young J.P.W. (1999): Molecular diversity of arbuscular mycorrhizal fungi colonising Hyacinthoides non-scripta (bluebell) in a seminatural woodland. Molecular Ecology, 8: 659–666.
Huang Y., Wang D.-W., Cai J.-L., Zheng W.-S. (2011): Review of glomalin-related soil protein and its environmental function in the rhizosphere. Chinese Journal of Plant Ecology, 35: 232–236.
Jakobsen I., Smith S.E., Smith F.A. (2002): Function and diversity of arbuscular mycorrhizae in carbon and mineral nutrition. In: van der Heijden M.G.A., Sanders I. (eds.): Mycorrhizal Ecology. Ecological Studies (Analysis and Synthesis), Vol. 157, Berlin, Heidelberg, Springer: 75–92.
Jones T.H., Thompson L.J., Lawton J.H., Bezemer T.M., Bardgett R.D., Blackburn T.M., Bruce K.D., Cannon P.F., Hall G.S., Hartley S.E., Howson G., Jones C.G., Kampichler C., Kandeler E., Ritchie D.A. (1998): Impacts of rising atmospheric carbon dioxideon model terrestrial ecosystems. Science, 280: 441–443.
Kagawa H.K., Osipiuk J., Maltsev N., Overbeek R., Quaite-Randall E., Joachimiak A., Trent J. D. (1995): The 60 kDa heat shock proteins in the hyperthermophilic archaeon Sulfolobus shibatae. Journal of Molecular Biology, 253: 712–725.
Kasurinen A., Helmisaari H.S., Holopainen T. (1999): The influence of elevated CO2 and O3 on fine roots and mycorrhizas of naturally growing young Scots pine trees during three exposure years. Global Change Biology, 5: 771–780.
Lovelock C.E., Wright S.F., Clark D.A., Ruess R.W. (2004): Soil stocks of glomalin produced by arbuscular mycorrhizal fungi across a tropical rain forest landscape. Journal of Ecology, 92: 278–287.
Lutgen E.R., Muir-Clairmont D., Graham J., Rillig M.C. (2003): Seasonality of arbuscular mycorrhizal hyphae and glomalin in a western Montana grassland. Plant and Soil, 257: 71–83.
Malekzadeh E., Aliasgharzad N., Majidi J., Abdolalizadeh J., Aghebati-Maleki L. (2016): Contribution of glomalin to Pb sequestration by arbuscular mycorrhizal fungus in a sand culture system with clover plant. European Journal of Soil Biology, 74: 45–51.
Miller R.M., Kling M. (2000): The importance of integration and scale in the arbuscular mycorrhizal symbiosis. Plant Soil, 226: 295–309.
Millner P.D., Wright S.F. (2002): Tools for support of ecological research on arbuscular mycorrhizal fungi. Symbiosis, 33: 101–123.
Mirás-Avalos J.M., Antunes P.M., Koch A., Khosla K., Klironomos J.N., Dunfield K.E. (2011): The influence of tillage on the structure of rhizosphere and root-associated arbuscular mycorrhizal fungal communities. Pedobiologia, 54: 235–241.
Nichols K.A., Wright S.F. (2004): Contributions of fungi to soil organic matter in agroecosystems. In: Magdoff F., Weil R.R. (eds.): Soil Organic Matter in Sustainable Agriculture. Florida, CRC: 179–198.
Nichols K.A., Wright S.F. (2005): Comparison of glomalin and humic acid in eight native US soils. Soil Science, 170: 985–997.
Plenchette C. (1983): Growth responses of several plant species to mycorrhizae in a soil of moderate P fertility. Plant and Soil, 70: 199–209.
Pohanka M., Vlček V. (2018): Assay of glomalin using a quartz crystal microbalance biosensor. Electroanalysis, 30: 453–458.
Read D.J. (1991): Mycorrhizas in ecosystems. Experientia, 47: 376–391.
Rillig M.C. (2004): Arbuscular mycorrhizae, glomalin, and soil aggregation. Canadian Journal of Soil Science, 84: 355–363.
Rillig M.C., Wright S.F., Nichols K.A., Schmidt W.F., Torn M.S. (2001): Large contribution of arbuscular mycorrhizal fungi to soil carbon pools in tropical forest soils. Plant and Soil, 233: 167–177.
Rillig M.C., Ramsey P.W., Morris S., Paul E.A. (2003): Glomalin, an arbuscular-mycorrhizal fungal soil protein, responds to land-use change. Plant and Soil, 253: 293–299.
Seguel A., Cumming J.R., Klugh-Stewart K., Cornejo P., Borie F. (2013): The role of arbuscular mycorrhizas in decreasing aluminium phytotoxicity in acidic soils: a review. Mycorrhiza, 23: 167–183.
Seguel A., Cumming J., Cornejo P., Borie F. (2016): Aluminum tolerance of wheat cultivars and relation to arbuscular mycorrhizal colonization in a non-limed and limed Andisol. Applied Soil Ecology, 108: 228–237.
Shi J.X., Fu M.J., Zhao C., Zhou F.L., Yang Q.B., Qiu L.H. (2016): Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon. Cell Stress Chaperones, 21: 295–312.
Singh P.K., Singh M., Tripathi B.N. (2013): Glomalin: an arbuscular mycorrhizal fungal soil protein. Protoplasma, 250: 663–669.
Smith S.E., Read D.J. (2008): Mycorrhizal Symbiosis. San Diego, Academic Press.
Smith S.E., Jakobsen I., Grønlund M., Smith F.A. (2011): Roles of arbuscular mycorrhizas in plant phosphorus nutrition: interactions between pathways of phosphorus uptake in arbuscular mycorrhizal roots have important implications for understanding and manipulating plant phosphorus acquisition. Plant Physiology, 156: 1050–1057.
St-Arnaud M., Hamel C., Vimard B., Caron M., Fortin J.A. (1996): Enhanced hyphal growth and spore production of the arbuscular mycorrhizal fungus Glomus intraradices in an in vitro system in the absence of host roots. Mycological research, 100: 328–332.
Steinberg P.D., Rillig M.C. (2003): Differential decomposition of arbuscular mycorrhizal fungal hyphae and glomalin. Soil Biology and Biochemistry, 35: 191–194.
Treseder K.K., Allen M.F. (2000): Mycorrhizal fungi have a potential role in soil carbon storage under elevated CO2 and nitrogen deposition. New Phytologist, 147: 189–200.
Treseder K.K., Turner K.M. (2007): Glomalin in ecosystems. Soil Science Society of America Journal, 71: 1257–1266.
Treseder K.K., Mack M.C., Cross A. (2004): Relationships among fires, fungi, and soil dynamics in Alaskan boreal forests. Ecological Applications, 14: 1826–1838.
van der Heijden M.G.A., Klironomos J.N., Ursic M., Moutoglis P., Streitwolf-Engel R., Boller T., Wiemken A., Sanders I.R. (1998): Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature, 396: 69–72.
Vodnik D., Grčman H., Maček I., Van Elteren J.T., Kovače-vič M. (2008): The contribution of glomalin-related soil protein to Pb and Zn sequestration in polluted soil. Science of the Total Environment, 392: 130–136.
Whipps J.M. (2004): Prospects and limitations for mycorrhizas in biocontrol of root pathogens. Canadian Journal of Botany, 82: 1198–1227.
Wright S.F., Upadhyaya A. (1998): A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi. Plant and Soil, 198: 97–107.
Wright S.F., Anderson R.L. (2000): Aggregate stability and glomalin in alternative crop rotations for the central Great Plains. Biology and Fertility of Soils, 31: 249–253.
Wright S.F., Nichols K. (2002): Glomalin: Hiding place for a third of the world’s stored soil carbon. Agricultural Research, 50: 4–7.
Wright S.F., Starr J.L., Paltineanu I.C. (1999): Changes in aggregate stability and concentration of glomalin during tillage management transition. Soil Science Society of America Journal, 63: 1825–1829.
Wuest S.B., Caesar-TonThat T.C., Wright S.F., Williams J.D. (2005): Organic matter addition, N, and residue burning effects on infiltration, biological, and physical properties of an intensively tilled silt–loam soil. Soil and Tillage Research, 84: 154–167.
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