Functional diversity of microorganisms in metal- and alkali-contaminated soils of Central and North-eastern Slovakia

https://doi.org/10.17221/37/2018-SWRCitation:Fazekaš J., Fazekašová D., Adamišin P., Huličová P., Benková E. (2019): Functional diversity of microorganisms in metal- and alkali-contaminated soils of Central and North-eastern Slovakia. Soil & Water Res., 14: 32-39.
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A field-based study and laboratory tests were undertaken to determine the functional diversity of microorganisms in metal- and alkali-contaminated soils in Central and North-eastern Slovakia where iron ore and magnesite have been mined and processed for a long time. To improve the understanding of the functional diversity of microorganisms, we examined the effects of environmental factors on the functional diversity of microorganisms in metal- and alkali-contaminated soils in the emission field of heaps and tailings impoundments of iron ore mines (Central Spiš) and magnesite factories (Jelšava and Lubeník). Biolog® Eco Plates were used to determine and assess metabolic profiles of microbial communities. The examined area of Central Spiš showed extremely high values of Hg and Cu and the values of Zn, Cd, Pb and Cr exceeding the permissible limit were determined. Soil reaction was extremely acidic to strongly acidic. The Jelšava and Lubeník area was characterized by alkaline contamination and the soil reaction was slightly acidic to strongly alkaline. The values of Cr, Mn, and Mg exceeding the permissible limit were measured there. The results indicate harmful and even toxic contamination. Our results showed that the diversity of microorganisms was different in the investigated areas and it was significantly influenced by environmental factors such as soil reaction, bulk density, porosity, and heavy metals Hg, Pb, Cr, Zn, Cu, Mn and Mg. Based on the results of the Shannon index, we can conclude that the diversity was low to moderate (2.5–3.3) and medium (3.3–4.0). Correlations between functional diversity of microorganisms and soil reaction, Hg, Cr, and Cu were determined. Our findings are decisive for understanding the microbial diversity in metal- and alkali-contaminated soils and they can be used to assess the quality and health of soil, as well as for scientific applications of remediation techniques.

 

References:
Act No. 220/2004 (2004): Coll. of Laws on the Protection and Use of Agricultural Land. Bratislava, National Council of Slovak Republic.
 
Angelovičová L., Fazekašová D. (2014): Contamination of the soil and water environment by heavy metals in the former mining area of Rudňany (Slovakia). Soil and Water Research, 9, 18-24 https://doi.org/10.17221/24/2013-SWR
 
Čurlík J., Šefčík P. (1999): Geochemical Atlas of the Slovak Republic. Bratislava, MŽP SR. (in Slovak)
 
Doelman P. (1985): Resistance of soil microbial communities to heavy metals. In: Jensen V., Kjoller A., Sorensen L.H. (eds.): Microbial Communities in Soil. London, Elsevier: 369–384.
 
Jansen E., Michels M., van Til M., Doelman P. (1994): Effects of heavy metals in soil on microbial diversity and activity as shown by the sensitivity-resistance index, an ecologically relevant parameter. Biology and Fertility of Soils, 17, 177-184 https://doi.org/10.1007/BF00336319
 
Fathizadeh M., Fakhraee H., Aroujalian A. (2011): Decontamination of hexavalent chromium and tri-ethyl phosphate stimulants through photacatalytic oxidation. International Journal of Environmental Science & Technology, 8: 863–871.
 
Fazekašová D. (2012): Evaluation of soil quality parameters development in terms of sustainable land use. In: Curkovic S. (ed.): Sustainable Development – Authoritative and Leading Edge Content for Environmental Management. Rijeka, InTech: 435-458.
 
Fazekašová D., Boguská Z., Fazekaš J., Škvareninová J., Chovancová J. (2016): Contamination of vegetation growing on soil and substrates in the unhygienic region of central Spiš (Slovakia) polluted by heavy metals. Journal of Environmental Biology, 37: 1335–1340.
 
Fiala K., Barančikova G., Brečkova V., Burik V., Houškova B., Chomaničova A., Kobza J., Litavec T., Makovnikova L., Pechova B., Varadiova D. (1999): Partial Monitoring System – Soil, Binding Methods. Bratislava, Research Institute of Soil Science and Conservation. (in Slovak)
 
Garland Jay L (1997): Analysis and interpretation of community-level physiological profiles in microbial ecology. FEMS Microbiology Ecology, 24, 289-300 https://doi.org/10.1111/j.1574-6941.1997.tb00446.x
 
He Huaidong, Tam Nora F. Y., Yao Aijun, Qiu Rongliang, Li Wai Chin, Ye Zhihong (2016): Effects of alkaline and bioorganic amendments on cadmium, lead, zinc, and nutrient accumulation in brown rice and grain yield in acidic paddy fields contaminated with a mixture of heavy metals. Environmental Science and Pollution Research, 23, 23551-23560 https://doi.org/10.1007/s11356-016-7593-1
 
Hohl H., Varma A. (2010): Soil: the living matrix. In: Sherameti I., Varma A. (eds.): Soil Heavy Metals, Soil Biology. Berlin, Heidelberg, Springer-Verlag: 1–18.
 
Hronec O., Vilček J., Andejovský P., Andrejovská A., Daňová M., Huttmanová E., Vilimová M., Škultéty P., Juhászová M. (2008): Heavy metals in plants and soils of Rudnianska-Gelnická loaded area. Acta regionalia et environmentalica, 12: 24–28.
 
Izah Sylvester Chibueze, Bassey Sunday Etim, Ohimain Elijah Ige (2017): Assessment of Pollution Load Indices of Heavy Metals in Cassava Mill Effluents Contaminated Soil: a Case Study of Small-scale Processors in a Rural Community in the Niger Delta, Nigeria. Bioscience Methods, , - https://doi.org/10.5376/bm.2017.08.0001
 
Jordan Gyozo, Abdaal Ahmed (2013): Decision support methods for the environmental assessment of contamination at mining sites. Environmental Monitoring and Assessment, 185, 7809-7832 https://doi.org/10.1007/s10661-013-3137-z
 
Kabata–Pendias A. (2011): Trace Elements in Soil and Plants. 4th Ed. London, CRC Press.
 
Kızılkaya Rıdvan, Aşkın Tayfun, Bayraklı Betül, Sağlam Mustafa (2004): Microbiological characteristics of soils contaminated with heavy metals. European Journal of Soil Biology, 40, 95-102 https://doi.org/10.1016/j.ejsobi.2004.10.002
 
Knight B.P., McGrath S.P., Chaudri A.M. (1997): Biomass carbon measurements and substrate utilization patterns of microbial populations from soils amended with cadmium, copper, or zinc. Applied and Environmental Microbiology, 63: 39–43.
 
Konopka A., Zakharova T., Bischoff M., Oliver L., Nakatsu C., Turco R.F. (1995): Microbial biomass and activity in lead-contaminated soil. Applied and Environmental Microbiology, 65: 2256–2259.
 
Liao Min, Xie Xiao M. (2007): Effect of heavy metals on substrate utilization pattern, biomass, and activity of microbial communities in a reclaimed mining wasteland of red soil area. Ecotoxicology and Environmental Safety, 66, 217-223 https://doi.org/10.1016/j.ecoenv.2005.12.013
 
Romaniuk Romina, Giuffré Lidia, Costantini Alejandro, Bartoloni Norberto, Nannipieri Paolo (2011): A comparison of indexing methods to evaluate quality of soils: the role of soil microbiological properties. Soil Research, 49, 733- https://doi.org/10.1071/SR11147
 
Ševčík P., Pramuka S., Gluch A. (2008): Assessment of soil contamination in Slovakia according index of geoaccumulation. Agriculture, 54: 119–130.
 
Shannon C. E. (1948): A Mathematical Theory of Communication. Bell System Technical Journal, 27, 379-423 https://doi.org/10.1002/j.1538-7305.1948.tb01338.x
 
Song Jing, Zhao Fang-Jie, McGrath Steve P., Luo Yong-Ming (2006): INFLUENCE OF SOIL PROPERTIES AND AGING ON ARSENIC PHYTOTOXICITY. Environmental Toxicology and Chemistry, 25, 1663- https://doi.org/10.1897/05-480R2.1
 
TENG Ying, LUO Yong-Ming, HUANG Chang-Yong, LONG Jian, LI Zhen-Gao, CHRISTIE P. (2008): Tolerance of Grasses to Heavy Metals and Microbial Functional Diversity in Soils Contaminated with Copper Mine Tailings. Pedosphere, 18, 363-370 https://doi.org/10.1016/S1002-0160(08)60026-0
 
Tischer S., Tannaberg H., Guggenberger G. (2008): Microbial parameters of soils contaminated with heavy metals: Assesment for ecotoxicological monitoring. Polish Journal of Ecology, 56: 471–479.
 
Torsvik Vigdis, Øvreås Lise (2002): Microbial diversity and function in soil: from genes to ecosystems. Current Opinion in Microbiology, 5, 240-245 https://doi.org/10.1016/S1369-5274(02)00324-7
 
Wang Lei, Tai Peidong, Jia Chunyun, Li Xiaojun, Li Peijun, Xiong Xianzhe (2015): Magnesium Contamination in Soil at a Magnesite Mining Region of Liaoning Province, China. Bulletin of Environmental Contamination and Toxicology, 95, 90-96 https://doi.org/10.1007/s00128-015-1530-8
 
Xie Xiaomei, Liao Min, Ma Aili, Zhang Haijun (2011): Effects of contamination of single and combined cadmium and mercury on the soil microbial community structural diversity and functional diversity. Chinese Journal of Geochemistry, 30, 366-374 https://doi.org/10.1007/s11631-011-0521-7
 
Zhu Li-xia, Xiao Qian, Shen Yu-fang, Li Shi-qing (2017): Microbial functional diversity responses to 2 years since biochar application in silt-loam soils on the Loess Plateau. Ecotoxicology and Environmental Safety, 144, 578-584 https://doi.org/10.1016/j.ecoenv.2017.06.075
 
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