Differences in humic acids structure of various soil types studied by DRIFT spectroscopy

https://doi.org/10.17221/76/2017-SWRCitation:Pavlů L., Mühlhanselová M. (2018): Differences in humic acids structure of various soil types studied by DRIFT spectroscopy. Soil & Water Res., 13: 29-35.
download PDF

The method of diffuse reflectance infrared spectroscopy (DRIFT) proved to be useful for studying the soil organic matter structure. The aim of this study was to compare DRIFT spectra of humic acids (HAs) separated from various soils and to identify their specific nature. Samples of agricultural soils (Dystric Cambisol, Haplic Chernozem, Greyic Phaeozem, and Haplic Luvisol) were collected from humic (A) horizons. Soil samples of reclaimed dumpsites (Haplic Technosol (molic) and (calcic)) were taken from the upper part of the soil profile. Samples of forest soils were collected from surface organic (H) horizons of Cambisols in spruce (Picea abies L. Karst.) and beech (Fagus silvatica L.) forests. The selected soil types differ in pH, carbon content, texture, and humus quality. An extraction of HAs was performed using a mixture of 0.5 M NaOH and 0.1 M Na4P2O7. The separated HAs were freeze-dried and analyzed using a DRIFT spectrometer (Thermo Nicolet Nexus) without KBr dilution. There were found differences in relative aromaticity among the agricultural soils HAs. The most aromatic character was observed in HAs from Chernozems and the least in Cambisols. Preservation of the natural structure of Chernozem HAs during the reclamation process was documented on the HAs spectra from Haplic Technosols (molic). By using the DRIFT spectra of HAs of forest H horizons, it is possible to identify the fragments of decomposed material (gymnosperms or angiosperms). HAs isolated from these horizons represent a certain interstage between lignin and the matured soil humic acids.

References:
Agnelli A., Celi L., Degl'Innocenti A., Corti G., Ugolini F. C. (2000): CHEMICAL AND SPECTROSCOPIC CHARACTERIZATION OF THE HUMIC SUBSTANCES FROM SANDSTONE-DERIVED ROCK FRAGMENTS. Soil Science, 165, 314-327  https://doi.org/10.1097/00010694-200004000-00003
 
Angst Gerrit, Mueller Kevin E., Kögel-Knabner Ingrid, Freeman Katherine H., Mueller Carsten W. (2017): Aggregation controls the stability of lignin and lipids in clay-sized particulate and mineral associated organic matter. Biogeochemistry, 132, 307-324  https://doi.org/10.1007/s10533-017-0304-2
 
Barthès Bernard, Roose Eric (2002): Aggregate stability as an indicator of soil susceptibility to runoff and erosion; validation at several levels. CATENA, 47, 133-149  https://doi.org/10.1016/S0341-8162(01)00180-1
 
Bonanomi Giuliano, Incerti Guido, Giannino Francesco, Mingo Antonio, Lanzotti Virginia, Mazzoleni Stefano (2013): Litter quality assessed by solid state 13C NMR spectroscopy predicts decay rate better than C/N and Lignin/N ratios. Soil Biology and Biochemistry, 56, 40-48  https://doi.org/10.1016/j.soilbio.2012.03.003
 
Boruvka Lubos, Mladkova Lenka, Drabek Ondrej (2005): Factors controlling spatial distribution of soil acidification and Al forms in forest soils. Journal of Inorganic Biochemistry, 99, 1796-1806  https://doi.org/10.1016/j.jinorgbio.2005.06.028
 
Casagrande A. (1934): Die Areometr Methode zur Bestimmung der Kornverteilung von Böden. Berlin, Springer.
 
Cerli C., Celi L., Kaiser K., Guggenberger G., Johansson M.-B., Cignetti A., Zanini E. (2008): Changes in humic substances along an age sequence of Norway spruce stands planted on former agricultural land. Organic Geochemistry, 39, 1269-1280  https://doi.org/10.1016/j.orggeochem.2008.06.001
 
Chai Xiaoli, Takayuki Shimaoka, Cao Xiaoyan, Guo Qiang, Zhao Youcai (2007): Spectroscopic studies of the progress of humification processes in humic substances extracted from refuse in a landfill. Chemosphere, 69, 1446-1453  https://doi.org/10.1016/j.chemosphere.2007.04.076
 
Chenu C., Le Bissonnais Y., Arrouays D. (2000): Organic Matter Influence on Clay Wettability and Soil Aggregate Stability. Soil Science Society of America Journal, 64, 1479-  https://doi.org/10.2136/sssaj2000.6441479x
 
Cunha T.J.F., Novotny E.H., Madari B.E., Martin-Neto L., Rezende M.O., Canelas L.P., Benites V.M. (2009): Spectroscopy Characterization of Humic Acids Isolated from Amazonian Dark Earth Soils (Terra Preta De Índio). In: Woods W.I. et al. (eds): Amazonian Dark Earths: Wim Sombroek’s Vision. Springer Science+Business Media B.V.: 363–372.
 
Ferrari Erika, Francioso Ornella, Nardi Serenella, Saladini Monica, Ferro Nicola Dal, Morari Francesco (2011): DRIFT and HR MAS NMR characterization of humic substances from a soil treated with different organic and mineral fertilizers. Journal of Molecular Structure, 998, 216-224  https://doi.org/10.1016/j.molstruc.2011.05.035
 
IUSS (2014): World Reference Base for Soil Resources. World Soil Resources Reports No. 106. Rome, FAO.
 
Kara O., Bolat I.K., Cakıroglu K., Senturk M. (2014): Litter decomposition and microbial biomass in temperate forests in Northwestern Turkey. Journal of Soil Science and Plant Nutrition, 14: 31–41.
 
Kočárek M., Kodešová R., Kozák J., Drábek O., Vacek O. (2005): Chlortoluron behavior in five different soil types. Plant Soil and Environment, 51: 304–309.
 
Kodešová Radka, Kočárek Martin, Klement Aleš, Golovko Oksana, Koba Olga, Fér Miroslav, Nikodem Antonín, Vondráčková Lenka, Jakšík Ondřej, Grabic Roman (2016): An analysis of the dissipation of pharmaceuticals under thirteen different soil conditions. Science of The Total Environment, 544, 369-381  https://doi.org/10.1016/j.scitotenv.2015.11.085
 
Liu Qian, Wang Shurong, Zheng Yun, Luo Zhongyang, Cen Kefa (2008): Mechanism study of wood lignin pyrolysis by using TG–FTIR analysis. Journal of Analytical and Applied Pyrolysis, 82, 170-177  https://doi.org/10.1016/j.jaap.2008.03.007
 
Mbagwu J.S.C., Piccolo A. (1998): Water-dispersible clay in aggregates of forest and cultivated soils in soutthern Nigeria in relation to organic matter constituents. In: Bergström L., Kirchman H. (eds): Carbon and Nutrient Dynamics in Natural and Agricultural Tropical Ecosystems. Oxford, CAB International: 71–83.
 
Mládková L., Rohošková M., Borůvka L. (2006): Methods for the assessment of humic substances quality in forest soils. Soil and Water Research, 1: 3–9.
 
Mühlhanselová M., Penížek V., Borůvka L. (2006): Study of Anthropogenic soils on a reclaimed dumpsite and their variability by geostatistical methods. Soil and Water Research, 1: 72–78.
 
Němeček J., Smolíková L., Kutílek M. (1990): Soil Science and Paleo Soil Science. Prague, Academia. (in Czech)
 
Piccolo Alessandro (2001): THE SUPRAMOLECULAR STRUCTURE OF HUMIC SUBSTANCES. Soil Science, 166, 810-832  https://doi.org/10.1097/00010694-200111000-00007
 
PICCOLO A., NARDI S., CONCHERI G. (1996): Macromolecular changes of humic substances induced by interaction with organic acids. European Journal of Soil Science, 47, 319-328  https://doi.org/10.1111/j.1365-2389.1996.tb01405.x
 
Piccolo A., Celano G., Conte P. (2000): Methods of isolation and characterization of humic substances to study their interactions with pesticides. In: Cornejo J., Jamet P. (eds): Pesticide/Soil Interactions. Paris, Institut National de la Recherche Agronomique (INRA):103–116.
 
Schulten Hans-Rolf, Schnitzer Morris (1997): Chemical Model Structures for Soil Organic Matter and Soils. Soil Science, 162, 115-130  https://doi.org/10.1097/00010694-199702000-00005
 
Sparks D.L. (1996): Methods of Soil Analysis. Part 3 – Chemical Methods. SSSA Book Series: 5, Madison, Soil Science Society of America, Inc. and American Society of Agronomy, Inc.
 
Sposito G. (2008): The Chemistry of Soils. Oxford, Oxford University Press, Inc.
 
Stevenson F.J. (1994): Humus Chemistry, Genesis, Composition, Reactions. 2nd Ed. New York, John Wiley and Sons, Inc.
 
Šarapatka B., Bednář M. (2015): Soil Science Days 2015. Czech and Slovac Soil Science in the International Year of Soil (Book of Abstracts). Olomouc, UPOL. (in Czech)
 
Tunega D., Aquino A.J.A., Haberhauer G., Lischka H., Schaumann G.E., Gerzabek M.H. (2014): Molecular models of cation and water molecule bridges in humic substances. In Hartemink A.E., McSweeney K. (eds): Soil Carbon. Cham, Springer International Publishing: 107–115.
 
download PDF

© 2020 Czech Academy of Agricultural Sciences