Pax5 as a potential candidate marker for canine B-cell lymphoma S., Techangamsuwan S., Tangkawattana S., Rungsipipat A. (2017): Pax5 as a potential candidate marker for canine B-cell lymphoma. Veterinarni Medicina, 62: 74-80.
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Immunophenotyping is a valuable method for prognosis in canine malignant lymphoma. The general B-cell marker is CD79a; however, Pax5 or B-cell specific activator protein, a transcription factor that controls B-cell identity and cell maturation, could also be used as a B-cell indicator in canine lymphomas. This study aimed to use Pax5, CD79a and CD3 expression in immunohistochemistry of spontaneous canine lymphomas, in order to carry out diagnosis and histopathological classification according to the World Health Organization guidelines. Forty-six retrospective cases including 33 multicentric, eight extranodal, and five alimentary lymphomas in dogs were immunostained by anti-Pax5 and anti-CD79a antibodies for B-cell identification, and anti-CD3 antibody for T-cell identification. T-cell lymphomas (CD3+/Pax5–/CD79a–) accounted for 30.43% of cases (14/46), and four of the lymphomas (28.57%) presented with CD3+/Pax5–/CD79a+. Conversely, B-cell lymphomas (CD3–/Pax5+/CD79a+) accounted for 69.57% of cases (32/46) and 12.5% of these (4/32) showed only Pax5-positive cells (CD3–/Pax5+/CD79a–). Therefore, in dogs, Pax5 appears to be a more useful marker for staining all B-cell subtypes compared to CD79a. Immunophenotyping with both Pax5 and CD3 are necessary for lymphoid lineage identification in canine lymphomas.
Agostinelli Claudio, Sabattini Elena, Gjørret Jakob Oemar, Righi Simona, Rossi Maura, Mancini Manuela, Piccaluga Pier Paolo, Bacci Francesco, Marafioti Teresa, Bettini Giuliano, Falini Brunangelo, Pileri Stefano A. (2010): Characterization of a New Monoclonal Antibody Against PAX5/BASP in 1525 Paraffin-embedded Human and Animal Tissue Samples. Applied Immunohistochemistry & Molecular Morphology, 18, 561-572
Browne Patrick, Petrosyan Karen, Hernandez Antonio, Chan Joel A. (2003): The B-Cell Transcription Factors BSAP, Oct-2, and BOB.1 and the Pan-B-Cell Markers CD20, CD22, and CD79a Are Useful in the Differential Diagnosis of Classic Hodgkin Lymphoma. American Journal of Clinical Pathology, 120, 767-777
Guija de Arespacochaga A., Schwendenwein I., Weissenböck H. (2007): Retrospective Study of 82 Cases of Canine Lymphoma in Austria based on the Working Formulation and Immunophenotyping. Journal of Comparative Pathology, 136, 186-192
Desouki M. M., Post G. R., Cherry D., Lazarchick J. (): PAX-5: A Valuable Immunohistochemical Marker in the Differential Diagnosis of Lymphoid Neoplasms. Clinical Medicine & Research, 8, 84-88
Dobson J. M., Samuel S., Milstein H., Rogers K., Wood J. L. N. (2002): Canine neoplasia in the UK: estimates of incidence rates from a population of insured dogs. Journal of Small Animal Practice, 43, 240-246
DVIR E., SCHOEMAN J. P., CLIFT S. J., McNEILLY T. N., MELLANBY R. J. (2011): Immunohistochemical characterization of lymphocyte and myeloid cell infiltrates in spirocercosis-induced oesophageal nodules. Parasite Immunology, 33, 545-553
Fernandes N. C.C. A., Guerra J. M., Réssio R. A., Wasques D. G., Etlinger-Colonelli D., Lorente S., Nogueira E., Dagli M. L. Z. (2016): Liquid-based cytology and cell block immunocytochemistry in veterinary medicine: comparison with standard cytology for the evaluation of canine lymphoid samples. Veterinary and Comparative Oncology, 14, 107-116
Fernandez V. (2005): Pathogenesis of Mantle-Cell Lymphoma: All Oncogenic Roads Lead to Dysregulation of Cell Cycle and DNA Damage Response Pathways. Journal of Clinical Oncology, 23, 6364-6369
Ferrer L., Fondevila D., Rabanal R., Tarres J., Ramis A. (1993): Immunohistochemical Detection of CD3 Antigen (Pan T Marker) in Canine Lymphomas. Journal of Veterinary Diagnostic Investigation, 5, 616-620
Fournel-Fleury C., Magnol J.P., Bricaire P., Marchal T., Chabanne L., Delverdier A., Bryon P.A., Felman P. (1997): Cytohistological and immunological classification of canine malignant lymphomas: Comparison with human non-Hodgkin's lymphomas. Journal of Comparative Pathology, 117, 35-59
Fournel-Fleury C., Ponce F., Felman P., Blavier A., Bonnefont C., Chabanne L., Marchal T., Cadore J. L., Goy-Thollot I., Ledieu D., Ghernati I., Magnol J. P. (2002): Canine T-cell Lymphomas: A Morphological, Immunological, and Clinical Study of 46 New Cases. Veterinary Pathology, 39, 92-109
Horcher Markus, Souabni Abdallah, Busslinger Meinrad (2001): Pax5/BSAP Maintains the Identity of B Cells in Late B Lymphopoiesis. Immunity, 14, 779-790
Jensen Kristin C, Higgins John P T, Montgomery Kelli, Kaygusuz Gulsah, van de Rijn Matt, Natkunam Yasodha (): The utility of PAX5 immunohistochemistry in the diagnosis of undifferentiated malignant neoplasms. Modern Pathology, 20, 871-877
Nasr M. R., Rosenthal N., Syrbu S. (): Expression Profiling of Transcription Factors in B- or T-Acute Lymphoblastic Leukemia/Lymphoma and Burkitt Lymphoma: Usefulness of PAX5 Immunostaining as Pan-Pre-B-Cell Marker. American Journal of Clinical Pathology, 133, 41-48
Ponce Frédérique, Magnol Jean-Pierre, Ledieu David, Marchal Thierry, Turinelli Vanessa, Chalvet-Monfray Karine, Fournel-Fleury Corinne (2004): Prognostic significance of morphological subtypes in canine malignant lymphomas during chemotherapy. The Veterinary Journal, 167, 158-166
Rebhun R. B., Kent M. S., Borrofka S. A. E. B., Frazier S., Skorupski K., Rodriguez C. O. (2011): CHOP chemotherapy for the treatment of canine multicentric T-cell lymphoma. Veterinary and Comparative Oncology, 9, 38-44
Regan R. C., Kaplan M. S. W., Bailey D. B. (2013): Diagnostic evaluation and treatment recommendations for dogs with substage-a high-grade multicentric lymphoma: results of a survey of veterinarians. Veterinary and Comparative Oncology, 11, 287-295
Rungsipipat Anudep, Chayapong Jutapon, Jongchalermchai Juliluck, Thongruk Thunchanok, Manachai Nawin, Wangnaitham Supradit, Techangamsuwan Somporn (2014): Histopathological classification and immunophenotyping of spontaneous canine lymphoma in Bangkok metropolitan. Comparative Clinical Pathology, 23, 213-222
Schaffer P. A., Charles J. B., Tzipory L., Ficociello J. E., Marvel S. J., Barrera J., Spraker T. R., Ehrhart E. J. (): Neurolymphomatosis in a Dog With B-Cell Lymphoma. Veterinary Pathology, 49, 771-774
Sirivisoot S, Techangamsuwan S, Tangkawattana S, Rungsipipat A (2016): Application of immunophenotyping and heteroduplex polymerase chain reaction (hPARR) for diagnosis of canine lymphomas. Asian Pacific Journal of Cancer Prevention 17, 2909–2916.
Stone Brett M., Gan David (2014): Application of the tissue transfer technique in veterinary cytopathology. Veterinary Clinical Pathology, 43, 295-302
Valli V. E., Myint M. S., Barthel A., Bienzle D., Caswell J., Colbatzky F., Durham A., Ehrhart E. J., Johnson Y., Jones C., Kiupel M., Labelle P., Lester S., Miller M., Moore P., Moroff S., Roccabianca P., Ramos-Vara J., Ross A., Scase T., Tvedten H., Vernau W. (): Classification of Canine Malignant Lymphomas According to the World Health Organization Criteria. Veterinary Pathology, 48, 198-211
Valli V. E., Kass P. H., Myint M. S., Scott F. (): Canine Lymphomas: Association of Classification Type, Disease Stage, Tumor Subtype, Mitotic Rate, and Treatment With Survival. Veterinary Pathology, 50, 738-748
Vezzali E., Parodi A. L., Marcato P. S., Bettini G. (2010): Histopathologic classification of 171 cases of canine and feline non-Hodgkin lymphoma according to the WHO. Veterinary and Comparative Oncology, 8, 38-49
Weiss Douglas J. (2006): A Retrospective Study of the Incidence and the Classification of Bone Marrow Disorders in the Dog at a Veterinary Teaching Hospital (1996-2004). Journal of Veterinary Internal Medicine, 20, 955-961
Wilkerson M.J., Dolce K., Koopman T., Shuman W., Chun R., Garrett L., Barber L., Avery A. (2005): Lineage differentiation of canine lymphoma/leukemias and aberrant expression of CD molecules. Veterinary Immunology and Immunopathology, 106, 179-196
Willmann M., Müllauer L., Guija de Arespacochaga A., Reifinger M., Mosberger I., Thalhammer J.G. (2009): Pax5 immunostaining in paraffin-embedded sections of canine non-Hodgkin lymphoma: A novel canine pan pre-B- and B-cell marker. Veterinary Immunology and Immunopathology, 128, 359-365
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