The effect of induced training on selected equine blood plasma indicators on treadmill trained horses

https://doi.org/10.17221/102/2020-VETMEDCitation:

Massanyi M, Halo Jr M, Kovacik A, Halo M, Imrich I, Formicki G, Mlynekova E, Massanyi P (2020): The effect of induced training on selected equine blood plasma indicators on treadmill trained horses. Vet Med-Czech 65, 528–536.

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The aim of this study was to evaluate the effect of induced training on the horses’ metabolism during an experiment lasting nine weeks where we continually scaled up the load on the horses by three defined stages. Blood was obtained from eighteen horses – two stallions, eight mares and eight geldings. In the experiment, we focused on the biochemical analysis of the blood plasma on multiple mineral profile indicators – Ca, P, Mg, K, Cl and Na, and some other variables (energy, nitrogen, AST, ALT, glucose, urea, creatinine kinase, total proteins). The result showed significant changes between the groups in most indicators. A significant increase in the potassium, phosphorus and calcium and a decrease in the concentrations of magnesium over the course of the experiment were found. For the other indicators, a significant increase in the activities of the AST and ALT out of the other indicators and the fluctuating values in the total proteins were noticed. Summarised, significant changes of multiple indicators were observed in different stages of the experiment. These changes had no visible effect on the horses’ organisms throughout entire duration of the experiment and were most probably caused by the muscular work and possible muscular damage during training.

References:
Art T, Amory H, Desmecht D, Lekeux P. Effect of show jumping on heart rate, blood lactate and other plasma biochemical values. Equine Vet J Suppl. 1990 Jun;(9):78-82. https://doi.org/10.1111/j.2042-3306.1990.tb04740.x
 
Balogh N, Gaal T, Ribiczeyne PS, Petri A. Biochemical and antioxidant changes in plasma and erythrocytes of pentathlon horses before and after exercise. Vet Clin Pathol. 2001;30(4):214-8. https://doi.org/10.1111/j.1939-165X.2001.tb00434.x
 
Dahalborn K, Jansson A, Nyman S, Lindholm A. Effects of dehydration and hyperhydration on fluid balance in the exercising Standardbred horse. Equine Res Centrum. 1996:52-7.
 
Halo M, Strapak P, Holly A, Mlynekova E, Kovalcik E, Horny M. Influence stress on the training process of the horses. J Cent Eur Agric. 2008 Apr 23;9(1):201-7.
 
Halo M, Holly A, Mlynekova E, Polyakova L, Horny M, Kovalcik E. Influence feeding and training on the metabolic profil sport horses. J Central Europ Agric. 2009 Nov 27;10(4):411-6.
 
Halo M, Tirpak F, Kovacik A, Komova P, Massanyi P. Correlations between motility parameters and biochemical parameters of stallion spermatozoa. In: Animal physiology; 2017 Jun 21–23; Stará Lesná: Slovak Academy of Sciences. 2017. p. 76.
 
Hinchcliff KW, Geor RJ. Integrative physiology of exercise. In: Hinchcliff KW, Kaneps AJ, Geor RJ, editors. Equine sports medicine and surgery. Edinburgh, London: Saunders Company; 2004. p. 3-8.
 
Jablonska EM, Ziolkowska SM, Gill J, Szykula R, Faff J. Changes in some haematological and metabolic indices in young horses during the first year of jump training. Equine Vet J. 1991 Jul;23(4):309-11. https://doi.org/10.1111/j.2042-3306.1991.tb03724.x
 
Johansson AM, Gardner SY, Jones SL, Faquay LR, Reagan VH, Levine JH. Hypomagnesemia in hospitalized horses. J Vet Intern Med. 2003 Nov-Dec;17(3):860-7. https://doi.org/10.1111/j.1939-1676.2003.tb02526.x
 
Jones EW. Scientific training. J Equine Vet Sci. 2005;25(7):320-1. https://doi.org/10.1016/j.jevs.2005.06.014
 
Kibler WB, Chandler TJ, Stracener ES. Musculoskeletal adaptations and injuries due to overtraining. Exerc Sport Sci Rev. 1992;20:1-99.
 
Kim JM, TaeYong K, Yoongjin C, Sejun O, Kabsu K, BumChul Y. The effect of horse riding simulator on energy expenditure, enjoyment and task difficulty in the elderly. Eur J Integr Med. 2016;8(5):723-30. https://doi.org/10.1016/j.eujim.2016.05.003
 
Kovacik A, Arvay J, Tusimova E, Harangozo L, Tvrda E, Zbynovska K, Cupka P, Andrascikova S, Tomas J, Massanyi P. Seasonal variations in the blood concentration of selected heavy metals in sheep and their effects on the biochemical and hematological parameters. Chemosphere. 2017 Feb;168:365-71. https://doi.org/10.1016/j.chemosphere.2016.10.090
 
Kovacik A, Tvrda E, Miskeje M, Arvay J, Tomka M, Zby-novska K, Andreji J, Hleba L, Kovacikova E, Fik M, Cupka P, Nahacky J, Massanyi P. Trace metals in the freshwater fish Cyprinus carpio: Effect to serum biochemistry and oxidative status markers. Biol Trace Elem Res. 2019 Apr;188(2):494-507. https://doi.org/10.1007/s12011-018-1415-x
 
Larsson J, Pilborg PH, Johansen M, Christophersen MT, Holte A, Roepstorff L, Olsen LH, Harrison AP. Physiological parameters of endurance horses pre- compared to post-race, correlated with performance: A two race study from scandinavia. ISRN Vet Sci. 2013 Sep 18;2013:684353. https://doi.org/10.1155/2013/684353
 
Lindinger MI, Geor RJ, Ecker GL, McCutcheon LJ. Plasma volume and ions during exercise in cool, dry; hot, dry; and hot, humid conditions. Equine Vet J Suppl. 1995 Nov;(20):133-9.  https://doi.org/10.1111/j.2042-3306.1995.tb05019.x
 
Manna I, Khanna GL, Dhara PC. Effect of training on physiological and biochemical variables of soccer players of different age groups. Asian J Sports Med. 2010;26(1):33-43. https://doi.org/10.5812/asjsm.34875
 
Massanyi P, Stawarz R, Halo M, Formicki G, Lukac N, Cup-ka P, Schwarcz P, Kovacik A, Tusimova E, Kovacik J. Blood concentration of copper, cadmium, zinc and lead in horses and its relation to hematological and biochemical parameters. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2014;49(8):973-9. https://doi.org/10.1080/10934529.2014.894322
 
McKeever KH, Schurg WA, Jarrett SH, Convertino VA. Exercise training-induced hypervolemia in the horse. Med Sci Sports Exerc. 1987 Feb;19(1):21-7. https://doi.org/10.1249/00005768-198702000-00005
 
Miglio A, Cappelli K, Capomaccio S, Mecocci S, Silvestrelli M, Antognoni MT. Metabolic and biomolecular changes induced by incremental long-term training in young thoroughbred racehorses during first workout season. Animals (Basel). 2020 Feb 18;10(2):317. https://doi.org/10.3390/ani10020317
 
Mlynekova E, Halo M, Marsalek M, Starostova L. Impact of training load on the heart rate of horses. Acta Fytotech Zootech. 2016;19(4):167-70. https://doi.org/10.15414/afz.2016.19.04.167-170
 
Nielsen BD, Potter GD, Greene LW, Morris L, Murray-Gerzik M, Smith WB, Martin MT. Response of young horses in training to varying concentration of dietary calcium and phosphorus. J Equine Vet Sci. 1998;18(6):397-404. https://doi.org/10.1016/S0737-0806(98)80586-8
 
Octura JER, Kyung-Joo L, Woo CH, Vega RSA, Choi J, Park J, Shin T, Cho S, Kim B, Cho B. Elevation of blood creatine kinase and selected blood parameters after exercise in thoroughbred racehorses (Equus caballus L.). J Res Agri Ani Sci. 2014;2(5):7-13.
 
Rose RJ, Allen JR, Hodgson DR, Stewart JH, Chan W. Responses to submaximal treadmill exercise and training in the horse: changes in haematology, arterial blood gas and acid base measurements, plasma biochemical values and heart rate. Vet Rec. 1983 Dec;113(26-27):612-8.
 
Schott HC 2nd, Marlin DJ, Geor RJ, Holbrook TC, Deaton CM, Vincent T, Dacre K, Schroter RC, Jose-Cunilleras E, Cornelisse CJ. Changes in selected physiological and laboratory measurements in elite horses competing in a 160 km endurance ride. Equine Vet J Suppl. 2006 Aug;(36):37-42. https://doi.org/10.1111/j.2042-3306.2006.tb05510.x
 
Siciliano PD, Lawrence LM, Danielsen K, Powell DM, Thompson KN. Effect of conditioning and exercise type on serum creatine kinase and aspartate aminotransferase activity. Equine Vet J. 1995;27(S18):243-7. https://doi.org/10.1111/j.2042-3306.1995.tb04929.x
 
Sommardabl CS, Andrews FM, Saxton AM, Geiser DR, Maykuth PL. Alterations in blood viscosity in horses competing in cross country jumping. Am J Vet Med Res. 1994 Mar;55(3):389-94.
 
Szarska E. Investigations of blood parameters for evaluation of health status and training effects in race and sport horses. AR Wroclaw. 2003:115.
 
Tyler-McGowan C, Golland L, Evans DL, Hodgson DR, Rose RJ. Haematological and biochemical responses to training and over training. Equine Vet J Supplement. 1999;31(S30):621-5. https://doi.org/10.1111/j.2042-3306.1999.tb05297.x
 
Valberg SJ, Mickelson JR, Gallant EM, Macleay JM, Lentz L, De La Corte F. Excertional rhabdomyolysis in quarter horses and thoroughbreds: one syndrome, multiple aetiologies. Equine Vet J Supplement. 1999 Jul;31(S30):533-8. https://doi.org/10.1111/j.2042-3306.1999.tb05279.x
 
Winnicka A. Reference values of basic laboratory tests in veterinary medicine. SGGW Warszawa, Poland. 2011;5:136.
 
Witkowska-Pilaszewicz O, Baska P, Czopowicz M, Zmigrodzka M, Szarska E, Szczepaniak J, Nowak Z, Winnicka A, Cywinska A. Anti-inflammatory state in Arabian horses introduced to the endurance training. Animals (Basel). 2019a Aug 27;9(9):616. https://doi.org/10.3390/ani9090616
 
Witkowska-Pilaszewicz O, Baska P, Czopowicz M, Zmigrodzka M, Szczepaniak J, Szarska E, Winnicka A, Cywinska A. Changes in serum amyloid A (SAA) concentration in Arabian endurance horses during first training season. Animals (Basel). 2019b Jun 8;9(6):330. https://doi.org/10.3390/ani9060330
 
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