Gram-positive aerobic and microaerophilic microorganisms isolated from pathological processes and lesions of horses

https://doi.org/10.17221/107/2016-VETMEDCitation:Bzdil J., Holy O., Chmelar D. (2017): Gram-positive aerobic and microaerophilic microorganisms isolated from pathological processes and lesions of horses. Veterinarni Medicina, 62: 1-9.
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The aim of this study was to characterise the genera and species of Gram-positive aerobic and microaerophilic microorganisms isolated from pathological processes and lesions in horses. In the period 2009–2014, 449 clinical samples from horses were examined. Of these, 229 (51%) were collected from the respiratory tract, 121 (26.9%) from the skin, 40 (8.9%) from the gastrointestinal tract, 40 (8.9%) from the eyes, 8 (1.8%) from the urinary tract, 6 (1.3%) from the musculoskeletal system, 4 (0.9%) from the lymphatic system and 1 (0.2%) from milk. The isolates were presumptively identified phenotypically, and identification was confirmed by molecular phenotypic MALDI-TOF. The most frequently detected strains (n = 330) were Staphylococcus spp., Streptococcus spp., Corynebacterium spp. with prevalence rates of 37.2%, 23.4% and 7.6%, respectively. In addition, 24 other taxa were identified, including Enterococcus spp., Bacillus spp., Trueperella pyogenes, Aerococcus viridans, Dermatophilus congolensis, Lysinibacillus fusiformis, Nocardiopsis alba and Streptomyces spp. Most of these are described as opportunistic pathogens of animals, including horses. Antibiotic susceptibility was tested using the disc diffusion method. Florfenicol and amoxycillin with clavulanic acid were the most effective antibiotics. The susceptibility to florfenicol was 100% for tested strains of Bacillus spp., Lysinibacillus spp., Corynebacterium spp., Dermatophilus congolensis, Streptococcus spp., Enterococcus spp., Aerococcus spp., Nocardiopsis alba and Trueperella pyogenes. The susceptibilities of Staphylococcus aureus and other staphylococci to florfenicol were 96.2% and 98.5% in tested strains, respectively. Amoxycillin with clavulanic acid exhibited 100% effectiveness against Corynebacterium spp., Dermatophilus congolensis, Streptococcus spp., Aerococcus spp., Enterococcus spp., Streptomyces spp., Nocardiopsis alba and Trueperella pyogenes tested strains. The susceptibilities of Staphylococcus aureus, other staphylococci and Bacillus/Lysinibacillus spp. to amoxycillin with clavulanic acid were 89.8%, 98.8% and 20.0% of tested strains, respectively.
References:
Bednarczyk A, Daczkowska-Kozon EG (2007): Pathogenic features of bacteria from the Bacillus cereus group (in Polish). Postepy Microbiologii 47, 51–63.
 
Bek-Thomsen M., Tettelin H., Hance I., Nelson K. E., Kilian M. (): Population Diversity and Dynamics of Streptococcus mitis, Streptococcus oralis, and Streptococcus infantis in the Upper Respiratory Tracts of Adults, Determined by a Nonculture Strategy. Infection and Immunity, 76, 1889-1896  https://doi.org/10.1128/IAI.01511-07
 
Bolin D. C., Donahue J. M., Vickers M. L., Harrison L., Sells S., Giles R. C., Hong C. B., Poonacha K. B., Roberts J., Sebastian M. M., Swerczek T. W., Tramontin R., Williams N. M. (2005): Microbiologic and Pathologic Findings in an Epidemic of Equine Pericarditis. Journal of Veterinary Diagnostic Investigation, 17, 38-44  https://doi.org/10.1177/104063870501700108
 
Erol E, Locke SJ, Donahoe JK, Mackin MA, Carter CN (2012a): Beta-hemolytic Streptococcus spp. from horses: a retrospective study (2000–2010). Journal of Veterinary Diagnostic Investigation 24, 142–147.
 
Erol E, Sells SF, Williams NM, Kennedy L, Locke SJ, Labeda DP, Donahue JM, Carter CN (2012b): An investigation of a recent outbreak of nocardioform placentitis caused abortions in horses. Veterinary Microbiology 158, 425–430.
 
Cochrane Christine A., Freeman Karen, Woods Emma, Welsby Sarah, Percival Steven L. (2009): Biofilm evidence and the microbial diversity of horse wounds. Canadian Journal of Microbiology, 55, 197-202  https://doi.org/10.1139/W08-115
 
Funke G, Von Gravenitz A, Clarridge JE, Bernard KA (1997): Clinical microbiology of coryneform bacteria. Clinical Microbiology Reviews 10, 125–159.
 
Herdan CL, Acke E, Dicken M, Archer RM, Forsyth SF, Gee EK, Pauwels FET (2012): Multi-drug-resistant Enterococcus spp. as a cause of non-responsive septic synovitis in three horses. New Zealand Veterinary Journal, 60, 297-304  https://doi.org/10.1080/00480169.2011.651702
 
Laus F., Preziuso S., Spaterna A., Beribè F., Tesei B., Cuteri V. (2007): Clinical and epidemiological investigation of chronic upper respiratory diseases caused by beta-haemolytic Streptococci in horses. Comparative Immunology, Microbiology and Infectious Diseases, 30, 247-260  https://doi.org/10.1016/j.cimid.2007.02.003
 
Leite A, Vinhas-Da-Silva A, Felicio L, Vilarinho AC, Ferreira G (2010): Aerococcus viridans urinary tract infection in a pediatric patient with secondary pseudohypoaldosteronism. Revista Argentina de Microbiologia 42, 269–270.
 
León-Galván Ma. Fabiola, Barboza-Corona José E., Lechuga-Arana A. Arianna, Valencia-Posadas Mauricio, Aguayo Daniel D., Cedillo-Pelaez Carlos, Martínez-Ortega Erika A., Gutierrez-Chavez Abner J. (2015): Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico. BioMed Research International, 2015, 1-9  https://doi.org/10.1155/2015/615153
 
Liu Gang, Liu Yongxia, Ali Tariq, Ferreri Miro, Gao Jian, Chen Wei, Yin Jinhua, Su Jingliang, Fanning Séamus, Han Bo, Woo Patrick CY (2015): Molecular and Phenotypic Characterization of Aerococcus viridans Associated with Subclinical Bovine Mastitis. PLOS ONE, 10, e0125001-  https://doi.org/10.1371/journal.pone.0125001
 
Masoud Wafa, Jakobsen Mogens (2003): Surface ripened cheeses: the effects of Debaryomyces hansenii, NaCl and pH on the intensity of pigmentation produced by Brevibacterium linens and Corynebacterium flavescens. International Dairy Journal, 13, 231-237  https://doi.org/10.1016/S0958-6946(02)00147-4
 
Minkin Ruth, Shapiro Janet M. (2004): Corynebacterium afermentans Lung Abscess and Empyema in a Patient with Human Immunodeficiency Virus Infection. Southern Medical Journal, 97, 395-397  https://doi.org/10.1097/01.SMJ.0000100114.54554.C1
 
Papadimitriou Konstantinos, Anastasiou Rania, Mavrogonatou Eleni, Blom Jochen, Papandreou Nikos C, Hamodrakas Stavros J, Ferreira Stéphanie, Renault Pierre, Supply Philip, Pot Bruno, Tsakalidou Effie (2014): Comparative genomics of the dairy isolate Streptococcus macedonicus ACA-DC 198 against related members of the Streptococcus bovis/Streptococcus equinus complex. BMC Genomics, 15, 272-  https://doi.org/10.1186/1471-2164-15-272
 
Paściak Mariola, Pawlik Krzysztof, Gamian Andrzej, Szponar Bogumiła, Skóra Justyna, Gutarowska Beata (): An airborne actinobacteria Nocardiopsis alba isolated from bioaerosol of a mushroom compost facility. Aerobiologia, , -  https://doi.org/10.1007/s10453-014-9336-4
 
Peyrou M, Higgins R, Lavoie JP (2003): Evolution of bacterial resistance to certain antibacterial agents in horses in a veterinary hospital. Canadian Veterinary Journal 44, 978–981.
 
Popescu GA, Benea E, Mitache E, Piper C, Horstkotte D (2005): An unusual bacterium, Aerococcus viridans, and four cases of infective endocarditis. The Journal of Heart Valve Disease 14, 317–319.
 
Quinn PJ, Carter ME, Markey B, Carter GR (eds) (1994): Clinical Veterinary Microbiology. 1st edn. Wolfe, London. 648 pp.
 
Rubin JE, Ball KR, Chirino-Trejo M (2011): Antimicrobial susceptibility of Staphylococcus aureus and Staphylococcus pseudintermedius isolated from various animals. Canadian Veterinary Journal 52, 153–157.
 
Silva N. (2001): Identification and antimicrobial susceptibility patterns of Staphylococcus spp. isolated from canine chronic otitis externa. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 53, 1-5  https://doi.org/10.1590/S0102-09352001000200001
 
Songer JG, Post KW (eds) (2005): Veterinary Microbiology, Bacterial and Fungal Agents of Animal Disease. Elsevier Saunders, St Louis. 434 pp.
 
Stull Jason W., Slavić Durda, Rousseau Joyce, Weese J. Scott (2014): Staphylococcus delphini and Methicillin-Resistant S. pseudintermedius in Horses, Canada. Emerging Infectious Diseases, 20, 485-487  https://doi.org/10.3201/eid2003.130139
 
Uwaezuoke JC, Aririatu LE (2004): A survey of antibiotic resistant Staphylococcus aureus strains from clinical sources in Owerri. Journal of Applied Sciences and Environmental Management 8, 67–69.
 
Wenzler Eric, Kamboj Kamal, Balada-Llasat Joan-Miquel (2015): Severe Sepsis Secondary to Persistent Lysinibacillus sphaericus, Lysinibacillus fusiformis and Paenibacillus amylolyticus Bacteremia. International Journal of Infectious Diseases, 35, 93-95  https://doi.org/10.1016/j.ijid.2015.04.016
 
Wintzer HJ (ed.) (1999): Horse Diseases, Study and Practice Guide (in Slovak). Slovak/Czech edition, Hajko and Hajkova, Bratislava. 538 pp.
 
Yildirim A.Ö, Lämmler Ch, Weiß R (2002): Identification and characterization of Streptococcus agalactiae isolated from horses. Veterinary Microbiology, 85, 31-35  https://doi.org/10.1016/S0378-1135(01)00481-3
 
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