Effect of melatonin on the gastric antioxidant defence in experimental burn trauma

https://doi.org/10.17221/109/2021-VETMEDCitation:

Hristova M, Tasinov O, Tzaneva M, Chivchibashi D, Kiselova-Kaneva Y, Bekyarova G (2022): Effect of melatonin on the gastric antioxidant defence in experimental burn trauma. Vet Med-Czech 67, 379–386.

download PDF

Severe burn trauma triggers oxidative gastric mucosal injury. The purpose of this study was to investigate the antioxidant defence mechanisms and protective effect of melatonin in the gastric mucosa after burn injury. In order to investigate the mechanisms involved in the gastric antioxidant defence in a rat burn model, quantitative real-time PCR and immunohistochemistry techniques were applied. An analysis of glutathione peroxidase 4 (GPx4), glutathione reductase (GR), and catalase (Cat) gene expression was performed along with the evaluation of the gastric Cu/Zn superoxide dismutase (Cu/Zn SOD) activity. Melatonin was applied immediately and 12 h after 30% of total body surface area burns. The burn injury significantly increased the Gpx4 mRNA (< 0.000 1) and Gsr mRNA (< 0.000 1) expression. It also had a slight positive effect on the Cat mRNA expression and Cu/Zn SOD activity. Melatonin, in turn, markedly augmented the burn-induced Cu/Zn SOD (< 0.000 1) activity, reversed the Gpx4 mRNA (< 0.000 1) and Gsr mRNA (< 0.000 1) expression, and inhibited the Cat mRNA level. In conclusion, the present study suggests that a burn injury adaptively increases the Cu/Zn SOD activity and enhances the Gpx4 and Gsr gene expression in the gastric mucosa. Melatonin effectively modulates the expression of the cellular antioxidant enzymes, and improves the antioxidant defence by augmenting the Cu/Zn SOD activity.

References:
AbuBakr HO, Aljuaydi SH, Abou-Zeid SM, El-Bahrawy A. Burn-induced multiple organ injury and protective effect of lutein in rats. Inflammation. 2018 Jun;41(3):760-72. https://doi.org/10.1007/s10753-018-0730-x
 
Bakkannavar SM, Nayak VC, Taravadi PV, Pradeep KG. Fatal curling’s ulcer after burns in a child – A case report. Med Leg Update. 2013;13(1):10-2.
 
Bandyopadhyay D, Biswas K, Bandyopadhyay U, Reiter RJ, Banerjee RK. Melatonin protects against stress-induced gastric lesions by scavenging the hydroxyl radical. J Pineal Res. 2000 Oct;29(3):143-51. Erratum in: J Pineal Res. 2000 Nov;29(4):248. https://doi.org/10.1034/j.1600-0633.2002.290408.x
 
Basak PY, Agalar F, Gultekin F, Eroglu E, Altuntas I, Agalar C. The effect of thermal injury and melatonin on incisional wound healing. Ulus Travma Acil Cerrahi Derg. 2003 Apr;9(2):96-101.
 
Bekyarova G, Galunska B, Ivanova D, Yankova T. Effect of melatonin on burn-induced gastric mucosal injury in rats. Burns. 2009 Sep;35(6):863-8. https://doi.org/10.1016/j.burns.2008.09.009
 
Cabeza J, Motilva V, Martin MJ, de la Lastra CA. Mechanisms involved in gastric protection of melatonin against oxidant stress by ischemia-reperfusion in rats. Life Sci. 2001 Feb 9;68(12):1405-15. https://doi.org/10.1016/S0024-3205(01)00935-3
 
Cevik O, Oba R, Macit C, Cetinel S, Kaya OT, Sener E, Sener G. Lycopene inhibits caspase-3 activity and reduces oxidative organ damage in a rat model of thermal injury. Burns. 2012 Sep;38(6):861-71. https://doi.org/10.1016/j.burns.2012.01.006
 
Costa EJ, Lopes RH, Lamy-Freund MT. Permeability of pure lipid bilayers to melatonin. J Pineal Res. 1995 Oct;19(3):123-6. https://doi.org/10.1111/j.1600-079X.1995.tb00180.x
 
Fadaak HA. Gastrointestinal hemorrhage in Burns patients – The experience of a burns unit in Saudi Arabia. Ann Burns Fire. 2000 Jun;13(2):81-3.
 
Fei ZW, Young VR, Lu XM, Rhodes AB, Tompkins RG, Fischman AJ, Yu YM. Burn injury differentially alters whole-blood and organ glutathione synthesis rates: An experimental model. Burns Trauma. 2013 Sep 18;1(2):87-94. https://doi.org/10.4103/2321-3868.118934
 
Feng JY, Chien JY, Kao KC, Tsai CL, Hung FM, Lin FM, Hu HC, Huang KL, Yu CJ, Yang KY. Predictors of early onset multiple organ dysfunction in major burn patients with ventilator support: Experience from a mass casualty explosion. Sci Rep. 2018 Jul 19;8(1):10939. https://doi.org/10.1038/s41598-018-29158-3
 
Hristova M, Bekyarova G, Tzaneva M. Heme oxygenase-1 and oxidative stress – Related markers in gastric mucosa in burns and protection with melatonin. Trakia J Sci. 2016;14(4):307-13. https://doi.org/10.15547/tjs.2016.04.001
 
Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria J Med. 2018 Dec;54(4):287-93. https://doi.org/10.1016/j.ajme.2017.09.001
 
Jacob S, Herndon DN, Hawkins HK, Enkhbaatar P, Cox RA. Xanthine oxidase contributes to sustained airway epithelial oxidative stress after scald burn. Int J Burns Trauma. 2017 Oct 25;7(6):98-106.
 
Kabasakal L, Sener G, Cetinel S, Contuk G, Gedik N, Yegen BC. Burn-induced oxidative injury of the gut is ameliorated by the leukotriene receptor blocker montelukast. Prostaglandins Leukot Essent Fatty Acids. 2005 Jun;72(6):431-40. https://doi.org/10.1016/j.plefa.2005.02.008
 
Kamada T, Sato N, Kawano S, Fusamoto H, Abe H. Gastric mucosal hemodynamics after thermal or head injury. A clinical application of reflectance spectrophotometry. Gastroenterology. 1982 Sep;83(3):535-40. https://doi.org/10.1016/S0016-5085(82)80187-X
 
Konturek PC, Duda A, Brzozowski T, Konturek SJ, Kwiecien S, Drozdowicz D, Pajdo R, Meixner H, Hahn EG. Activation of genes for superoxide dismutase, interleukin-1beta, tumor necrosis factor-alpha, and intercellular adhesion molecule-1 during healing of ischemia-reperfusion-induced gastric injury. Scand J Gastroenterol. 2000 May;35(5):452-63. https://doi.org/10.1080/003655200750023697
 
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods. 2001 Dec;25(4):402-8. https://doi.org/10.1006/meth.2001.1262
 
Lushchak VI. Glutathione homeostasis and functions: Potential targets for medical interventions. J Amino Acids. 2012 Feb;2012: [26]. https://doi.org/10.1155/2012/736837
 
Saitoh D, Okada Y, Takahara T, Yamashita H, Ohno H, Inoue M. The effect of an SOD derivative (SM-SOD) administration in a burned rat model. Tohoku J Exp Med. 1994 Sep;174(1):31-40. https://doi.org/10.1620/tjem.174.31
 
Saitoh D, Shirani KZ, Cioffi WG, Kizaki T, Ohno H, Okada Y, Mason AD Jr, Pruitt BA Jr. Changes in the tissue and plasma superoxide dismutase (SOD) levels in a burned rat model. Tohoku J Exp Med. 2001 Jan;193(1):27-36. https://doi.org/10.1620/tjem.193.27
 
Sehirli O, Sener E, Sener G, Cetinel S, Erzik C, Yegen BC. Ghrelin improves burn-induced multiple organ injury by depressing neutrophil infiltration and the release of pro-inflammatory cytokines. Peptides. 2008 Jul;29(7):1231-40. https://doi.org/10.1016/j.peptides.2008.02.012
 
Sener G, Sehirli AO, Satiroglu H, Keyer-Uysal M, Yegen BC. Melatonin improves oxidative organ damage in a rat model of thermal injury. Burns. 2002 Aug;28(5):419-25. https://doi.org/10.1016/S0305-4179(02)00053-0
 
Szczesny B, Brunyanszki A, Ahmad A, Olah G, Porter C, Toliver-Kinsky T, Sidossis L, Herndon DN, Szabo C. Time-dependent and organ-specific changes in mitochondrial function, mitochondrial DNA integrity, oxidative stress and mononuclear cell infiltration in a mouse model of burn injury. PLoS One. 2015 Dec 2;10(12):e0143730. https://doi.org/10.1371/journal.pone.0143730
 
Tan DX, Manchester LC, Esteban-Zubero E, Zhou Z, Reiter RJ. Melatonin as a potent and inducible endogenous antioxidant: Synthesis and metabolism. Molecules. 2015 Oct 16;20(10):18886-906. https://doi.org/10.3390/molecules201018886
 
Toklu HZ, Tunali-Akbay T, Erkanli G, Yuksel M, Ercan F, Sener G. Silymarin, the antioxidant component of Silybum marianum, protects against burn-induced oxidative skin injury. Burns. 2007 Nov;33(7):908-16. https://doi.org/10.1016/j.burns.2006.10.407
 
Zhu L, Yang ZC, Li A, Cheng DC. Reduced gastric acid production in burn shock period and its significance in the prevention and treatment of acute gastric mucosal lesions. World J Gastroenterol. 2000 Feb;6(1):84-8. https://doi.org/10.3748/wjg.v6.i1.84
 
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti