Gram-negative aerobic and microaerophilic microorganisms isolated from pathological processes and lesions of horses

https://doi.org/10.17221/117/2017-VETMEDCitation:Bzdil J, Holy O, Toporcak J (2018): Gram-negative aerobic and microaerophilic microorganisms isolated from pathological processes and lesions of horses. Veterinarni Medicina, 63: 55-62.
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The pathogenicity of bacterial strains isolated from pathological processes and lesions of horses, strategies for their treatment and the choice of appropriate antimicrobials are frequently a challenging problem for private veterinarians who seek help in our laboratory. Therefore, the aim of this study was to map genera and species of Gram-negative aerobic and microaerophilic microorganisms isolated from pathological processes in horses and to identify the most effective antimicrobial agents for therapy based on antibiotic susceptibility. Between 2009 and 2014 a total of 449 clinical samples (n = 449) were examined; 229 (51%) of them were obtained from the respiratory tract, 121 (27%) from the skin, 40 (8.9%) from the digestive tract, 40 (8.9%) from the eyes, eight (1.8%) from the urinary system, six (1.3%) from the musculoskeletal system, four (0.9%) from the lymphatic system and one (0.2%) from milk. The examination was performed using conventional microbiological culture methods. The identification of isolates was confirmed using MALDI-TOF molecular phenotyping (Bruker Daltonics GmbH, Bremen, Germany). From the 276 Gram-negative isolates (prevalence of 61.5%), the most frequently detected strains were Enterobacter spp., Escherichia spp., Acinetobacter spp., Pseudomonas spp. and Actinobacillus spp. with prevalence rates of 7.6%, 6.7%, 6.7%, 6.0% and 5.8%. In addition, another 20 genera of microorganisms were detected. Susceptibility to antimicrobial agents was determined using the disc diffusion method. The most effective agents were gentamicin (94.1%), enrofloxacin (91.7%), colistin (87.0%), florfenicol (86.2%), neomycin (85.5%), streptomycin (82.4%) and tetracycline (78.5%). A good knowledge of the spectrum of bacterial species participating in pathological processes and lesions in horses and their antimicrobial susceptibility may be of great importance not only in treatment but also in deciding which prophylactic antibiotics to administer after surgical interventions.
References:
Berger S, Fröhlich W (2011): Stenotrophomonas maltophilia as a causative agent of bacterial infections in the horse: two clinical cases. Pferdeheilkunde Equine Medicine, 27, 381-385 https://doi.org/10.21836/PEM20110403
 
BERNARDET J.-F., SEGERS P., VANCANNEYT M., BERTHE F., KERSTERS K., VANDAMME P. (1996): Cutting a Gordian Knot: Emended Classification and Description of the Genus Flavobacterium, Emended Description of the Family Flavobacteriaceae, and Proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). International Journal of Systematic Bacteriology, 46, 128-148 https://doi.org/10.1099/00207713-46-1-128
 
Boguta L., Gradzki Z., Borges E., Maurin F., Kodjo A., Winiarczyk S. (2002): Bacterial Flora in Foals with Upper Respiratory Tract Infections in Poland. Journal of Veterinary Medicine Series B, 49, 294-297 https://doi.org/10.1046/j.1439-0450.2002.00570.x
 
Bzdil J (2003): Determination of Susceptibility of Microorganisms to Antimicrobials by Disc Diffusion Method. Standard Operating Procedure SOP BAK 10/03, SVI Olomouc. p 4.
 
Bzdil J., Holy O., Chmelar D. (2017): Gram-positive aerobic and microaerophilic microorganisms isolated from pathological processes and lesions of horses. Veterinární Medicína, 62, 1-9 https://doi.org/10.17221/107/2016-VETMED
 
CLSI – Clinical and Laboratory Standards Institute (2016): Performance Standards for Antimicrobial Susceptibility Testing– M100-S26. Twenty-six informational supplement. Wayne, Pensylvania. 296 pp.
 
Dominguez M., Münstermann S., de Guindos I., Timoney P. (2016): Equine disease events resulting from international horse movements: Systematic review and lessons learned. Equine Veterinary Journal, 48, 641-653 https://doi.org/10.1111/evj.12523
 
Furr M, Mogg TD (2003): Antimicrobial treatment of neonatal foals. Compendium 25, 302–309.
 
Hsueh Pei-Tan, Liu Chiu-Lin, Wang Hsuan-Han, Ni Wei-Fen, Chen Ya-Lei, Liu Jong-Kang (2016): A comparison of the immunological potency of Burkholderia lipopolysaccharides in endotoxemic BALB/c mice. Microbiology and Immunology, 60, 725-739 https://doi.org/10.1111/1348-0421.12450
 
Kinoshita Yuta, Niwa Hidekazu, Katayama Yoshinari (2015): Use of loop-mediated isothermal amplification to detect six groups of pathogens causing secondary lower respiratory bacterial infections in horses. Microbiology and Immunology, 59, 365-370 https://doi.org/10.1111/1348-0421.12257
 
Liu Huimin, Yan Jing, Wang Yutian, Yan Qi, Zhao Linping, Yan Ruoqian, He Hongxuan (2014): Isolation of Moraxella bovoculi from racehorses with keratoconjunctivitis. Journal of Veterinary Diagnostic Investigation, 26, 585-587 https://doi.org/10.1177/1040638714535601
 
Murphy Timothy F., Faden Howard, Bakaletz Lauren O., Kyd Jennelle M., Forsgren Arne, Campos Jose, Virji Mumtaz, Pelton Stephen I. (2009): Nontypeable Haemophilus influenzae as a Pathogen in Children. The Pediatric Infectious Disease Journal, 28, 43-48 https://doi.org/10.1097/INF.0b013e318184dba2
 
Onishi Janet C., Park Joong-Wook, Häggblom Max M., Fennell Michael J., Fugaro Michael N. (2012): Chronic laminitis is associated with potential bacterial pathogens in the laminae. Veterinary Microbiology, 158, 329-336 https://doi.org/10.1016/j.vetmic.2012.02.024
 
Quinn PJ, Markey BK, Leonard FC, FitzPatrick ES, Fanning S, Hartigan PJ (2011): Veterinary Microbiology and Microbial Disease. 2nd edn. Blackwell Publishing, Oxford. 912 pp.
 
Ruggiero Nicole A, Chang Mei, Brown Sheldon (2016): Pseudomonas mendocina Bacteremia, A Case Study and Review of Literature. Infectious Diseases in Clinical Practice, 24, 314-317 https://doi.org/10.1097/IPC.0000000000000394
 
Waldridge Bryan M., Stewart Allison J., Taylor Debra C., Saville William J. (2011): The Incidence of Aeromonas Species in the Feces of Nondiarrheic Horses. Journal of Equine Veterinary Science, 31, 700-702 https://doi.org/10.1016/j.jevs.2011.05.004
 
Wintzer HJ (1999): Horse diseases, study and practice guide. Slovak/Czech edition. Hajko and Hajkova, Bratislava. 538 pp.
 
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