Post-antibiotic effect of marbofloxacin, enrofloxacin and amoxicillin against selected respiratory pathogens of pigs

https://doi.org/10.17221/123/2018-VETMEDCitation:Nedbalcova K., Zouharova M., Sperling D. (2019): Post-antibiotic effect of marbofloxacin, enrofloxacin and amoxicillin against selected respiratory pathogens of pigs. Veterinarni Medicina, 64: 67-77.
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The post-antibiotic effect is defined as the period of bacterial growth suppression that persists after a limited exposure of organisms to antimicrobials and knowledge of its duration is important in establishing and optimising current dosing schedules for the treatment of bacterial infections. The post-antibiotic effect of marbofloxacin, enrofloxacin and amoxicillin were evaluated in vitro for Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida strains which originated from clinical samples of diseased pigs and were confirmed as susceptible to all tested antimicrobials based on determination of minimal inhibitory concentrations. The post-antibiotic effect for individual antimicrobials was monitored at five and ten times minimum inhibitory concentrations for one and two hours. The duration of the post-antibiotic effect for tested antimicrobials was found to exhibit the following order for all tested pathogens: marbofloxacin > enrofloxacin > amoxicillin. The longest duration of post-antibiotic effect of all tested antimicrobials was found in A. pleuropneumoniae and the shortest post-antibiotic effect duration was detected in P. multocida. No statistical differences in post-antibiotic effect duration were found within marbofloxacin and enrofloxacin in A. pleuropneumoniae and H. parasuis strains. In P. multocida strains there was a statistically significant difference (P = 0.0189). On the other hand, the differences between amoxicillin and marbofloxacin or enrofloxacin were statistically significant in all cases (P-values ranged between 0.0058 and 0.008). The prolonged post-antibiotic effect of fluoroquinolones and amoxicillin on important Gram-negative swine pathogens was confirmed. The results can be used to clarify the effect and mechanism of action of antimicrobial drugs in veterinary medicine.

References:
Aarestrup Frank M, Seyfarth Anne Mette, Angen Øystein (2004): Antimicrobial susceptibility of Haemophilus parasuis and Histophilus somni from pigs and cattle in Denmark. Veterinary Microbiology, 101, 143-146 https://doi.org/10.1016/j.vetmic.2004.02.012
 
Anadon A, Martinez-Larranaga MR, Diaz MJ, Fernandez-Cruz MI, Martinez MA, Frejo MT, Martinez M, Iturbe J, Tafur M (1999): Pharmacokinetic variables and tissue residues of enrofloxacin and ciprofloxacin in healthy pigs. American Journal of Veterinary Research 60, 1377–1382.
 
Biberstein EL (1990): Our understanding of the Pasteurellaceae. Canadian Journal of Veterinary Research 54, 78–82.
 
Burch DGS (2010): Antimicrobial concentrations in plasma and lung and their relationships to bacterial respiratory infections. The Pig Journal 63, 34–49.
 
CAI X, CHEN H, BLACKALL P, YIN Z, WANG L, LIU Z, JIN M (2005): Serological characterization of isolates from China. Veterinary Microbiology, 111, 231-236 https://doi.org/10.1016/j.vetmic.2005.07.007
 
Carbone Maria, Pennisi Maria Grazia, Masucci Marisa, De Sarro Angela, Giannone Michela, Fera Maria Teresa (2001): Activity and postantibiotic effect of marbofloxacin, enrofloxacin, difloxacin and ciprofloxacin against feline Bordetella bronchiseptica isolates. Veterinary Microbiology, 81, 79-84 https://doi.org/10.1016/S0378-1135(01)00349-2
 
Cester C.C., Toutain P.L. (1997): A Comprehensive Model for Enrofloxacin to Ciprofloxacin Transformation and Disposition in Dog. Journal of Pharmaceutical Sciences, 86, 1148-1155 https://doi.org/10.1021/js9603461
 
CLSI – Clinical and Laboratory Standards Institute (2013a): Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard – Fourth Edition. CLSI document Vet 01-A4. Clinical and Laboratory Standards Institute, Wayne. 73 pp.
 
CLSI – Clinical and Laboratory Standards Institute (2013b): Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Second Informational Supplement. CLSI document Vet 01-S2. Clinical and Laboratory Standards Institute, Wayne. 63 pp.
 
Craig W. A. (1993): Post-antibiotic effects in experimental infection models: relationship to in-vitro phenomena and to treatment of infections in man. Journal of Antimicrobial Chemotherapy, 31, 149-158 https://doi.org/10.1093/jac/31.suppl_D.149
 
Craig WA, Gudmundsson S (1996): The postantibiotic effect. In: Lorian V (ed.): Antibiotics in Laboratory Medicine. 4th edn. The Williams & Wilkins Co. 296–329.
 
Craig WA, Vogelman B (1986): The postantibiotic effect. Annals of Internal Medicine 106, 900–902.
 
de Jong Anno, Thomas Valérie, Simjee Shabbir, Moyaert Hilde, El Garch Farid, Maher Kirsty, Morrissey Ian, Butty Pascal, Klein Ulrich, Marion Hervé, Rigaut Delphine, Vallé Michel (2014): Antimicrobial susceptibility monitoring of respiratory tract pathogens isolated from diseased cattle and pigs across Europe: The VetPath study. Veterinary Microbiology, 172, 202-215 https://doi.org/10.1016/j.vetmic.2014.04.008
 
de la Fuente A.J. Martín, Tucker A.W., Navas J., Blanco M., Morris S.J., Gutiérrez-Martín C.B. (2007): Antimicrobial susceptibility patterns of Haemophilus parasuis from pigs in the United Kingdom and Spain. Veterinary Microbiology, 120, 184-191 https://doi.org/10.1016/j.vetmic.2006.10.014
 
El Garch Farid, de Jong Anno, Simjee Shabbir, Moyaert Hilde, Klein Ulrich, Ludwig Carolin, Marion Hervé, Haag-Diergarten Silke, Richard-Mazet Alexandra, Thomas Valérie, Siegwart Ed (2016): Monitoring of antimicrobial susceptibility of respiratory tract pathogens isolated from diseased cattle and pigs across Europe, 2009–2012: VetPath results. Veterinary Microbiology, 194, 11-22 https://doi.org/10.1016/j.vetmic.2016.04.009
 
El Garch F., Kroemer S., Galland D., Morrissey I., Woehrle F. (2017): Survey of susceptibility to marbofloxacin in bacteria isolated from diseased pigs in Europe. Veterinary Record, 180, 591-591 https://doi.org/10.1136/vr.103954
 
Garcia Ovando H., Gorla N., Luders C., Poloni G., Errecalde C., Prieto G., Puelles I. (1999): Comparative pharmacokinetics of enrofloxacin and ciprofloxacin in chickens. Journal of Veterinary Pharmacology and Therapeutics, 22, 209-212 https://doi.org/10.1046/j.1365-2885.1999.00211.x
 
Gerber A. U., Craig W. A. (1981): Growth kinetics of respiratory pathogens after short exposures to ampicillin and erythromycin in vitro. Journal of Antimicrobial Chemotherapy, 8, 81-91 https://doi.org/10.1093/jac/8.suppl_C.81
 
Gould IM, Milne K, Jason C (1991): Post antibiotic effect of co-amoxyclav. In: 5th European Congress on Clinical Microbiology and Infectious Disease. European Society of Clinical Microbiology and Infection Diseases. September 9.−11. 1991. Abstract 1360.
 
Hao H., Pan H., Ahmad I., Cheng G., Wang Y., Dai M., Tao Y., Chen D., Peng D., Liu Z., Huang L., Yuan Z. (2013): Susceptibility Breakpoint for Enrofloxacin against Swine Salmonella spp.. Journal of Clinical Microbiology, 51, 3070-3072 https://doi.org/10.1128/JCM.01096-13
 
Harada Kazuki, Shimizu Takae, Kataoka Yasushi, Takahashi Toshio (2012): Post-antibiotic effect of orbifloxacin against Escherichia coli and Pseudomonas aeruginosa isolates from dogs. Acta Veterinaria Scandinavica, 54, - https://doi.org/10.1186/1751-0147-54-16
 
Hossain Md. Akil, Park Hae-chul, Jeong Kyunghun, Jang Yang ho, Kim Dae Gyun, Kang JeongWoo, Lee Kwang-jick (2017): Pharmacokinetic and Pharmacodynamic Evaluation of Marbofloxacin in Pig against Korean Local Isolates of Actinobacillus pleuropneumoniae. BioMed Research International, 2017, 1-11 https://doi.org/10.1155/2017/2469826
 
Idowu O.R., Peggins J.O., Cullison R., Bredow J. von (2010): Comparative pharmacokinetics of enrofloxacin and ciprofloxacin in lactating dairy cows and beef steers following intravenous administration of enrofloxacin. Research in Veterinary Science, 89, 230-235 https://doi.org/10.1016/j.rvsc.2009.12.019
 
Licata L, Smith C E, Goldschmidt R M, Barrett J F, Frosco M (1997): Comparison of the postantibiotic and postantibiotic sub-MIC effects of levofloxacin and ciprofloxacin on Staphylococcus aureus and Streptococcus pneumoniae.. Antimicrobial Agents and Chemotherapy, 41, 950-955 https://doi.org/10.1128/AAC.41.5.950
 
MESSENGER K. M., PAPICH M. G., BLIKSLAGER A. T. (2012): Distribution of enrofloxacin and its active metabolite, using an in vivo ultrafiltration sampling technique after the injection of enrofloxacin to pigs. Journal of Veterinary Pharmacology and Therapeutics, 35, 452-459 https://doi.org/10.1111/j.1365-2885.2011.01338.x
 
Nedbalcová Kateřina, Kučerová Zdeňka (2013): Antimicrobial susceptibility of Pasteurella multocida and Haemophilus parasuis isolates associated with porcine pneumonia. Acta Veterinaria Brno, 82, 3-7 https://doi.org/10.2754/avb201382010003
 
Nedbalcova K., Satran P., Jaglic Z., Ondriasova R., Kucerova Z. (2012): Haemophilus parasuis and Glässer’s disease in pigs: a review. Veterinární Medicína, 51, 168-179 https://doi.org/10.17221/5537-VETMED
 
Nedbalcova K., Nechvatalova K., Pokludova L., Bures J., Kucerova Z., Koutecka L., Hera A. (2014): Resistance to selected beta-lactam antibiotics. Veterinary Microbiology, 171, 328-336 https://doi.org/10.1016/j.vetmic.2014.02.004
 
Nedbalcová Kateřina, Zouharová Monika, Šperling Daniel (2017): The determination of minimum inhibitory concentrations of selected antimicrobials for porcine Haemophilus parasuis isolates from the Czech Republic. Acta Veterinaria Brno, 86, 175-181 https://doi.org/10.2754/avb201786020175
 
Nouws J. F. M., Mevius D. J., Vree T. B., Baars A. M., Laurensen J. (1988): Pharmacokinetics, renal clearance and metabolism of ciprofloxacin following intravenous and oral administration to calves and pigs. Veterinary Quarterly, 10, 156-163 https://doi.org/10.1080/01652176.1988.9694165
 
Rao G.S., Ramesh S., Ahmad A.H., Tripathi H.C., Sharma L.D., Malik J.K. (2002): Pharmacokinetics of Enrofloxacin and its Metabolite Ciprofloxacin in Goats given Enrofloxacin Alone and in Combination with Probenecid. The Veterinary Journal, 163, 85-93 https://doi.org/10.1053/tvjl.2001.0594
 
Rescott D L, Nix D E, Holden P, Schentag J J (1988): Comparison of two methods for determining in vitro postantibiotic effects of three antibiotics on Escherichia coli.. Antimicrobial Agents and Chemotherapy, 32, 450-453 https://doi.org/10.1128/AAC.32.4.450
 
Sweeney MT, Lindeman C, Johansen L, Mullins L, Murray R, Senn MK, Bade D, Maqchin C, Kotarski SF, Tiwari R (2017): Antimicrobial susceptibility of Actinobacillus pleuropneumoniae, Pasteurella multocida, Streptococcus suis, and Bordetella bronchiseptica isolated from pigs in the United States and Canada, 2011 to 2015. Journal of Swine Health and Production 25, 106–120.
 
Tanigawa M., Sawada T. (2002): Effects of Subminimal Inhibitory Concentrations of Amoxicillin against Actinobacillus pleuropneumoniae. Journal of Veterinary Medicine Series B, 49, 513-518 https://doi.org/10.1046/j.1439-0450.2002.00604.x
 
Thorburn CE, Moleswoth SJ (1992): The post β-lactamase inhibitor effect (PLIE): a novel aspect of the activity of clavulanic acid in antibacterial tests. In: 32nd Interscience Conference on Antimicrobial Agents and Chemotherapy. American Society for Microbiology. October 11.−14. 1992. Abstract 540.
 
Thorburn C E, Molesworth S J, Sutherland R, Rittenhouse S (1996): Postantibiotic and post-beta-lactamase inhibitor effects of amoxicillin plus clavulanate.. Antimicrobial Agents and Chemotherapy, 40, 2796-2801 https://doi.org/10.1128/AAC.40.12.2796
 
Trouchon Tessa, Lefebvre Sébastien (2016): A Review of Enrofloxacin for Veterinary Use. Open Journal of Veterinary Medicine, 06, 40-58 https://doi.org/10.4236/ojvm.2016.62006
 
Wang Jianyi, Hao Haihong, Huang Lingli, Liu Zhenli, Chen Dongmei, Yuan Zonghui (2016): Pharmacokinetic and Pharmacodynamic Integration and Modeling of Enrofloxacin in Swine for Escherichia coli. Frontiers in Microbiology, 7, - https://doi.org/10.3389/fmicb.2016.00036
 
Wetzstein HG (1994): The in vitro postantibiotic effect of enrofloxacin. Proceedings of the 18th World Buiatrics Congress, 615–618.
 
Zhao D.H., Yu Y., Zhou Y.F., Shi W., Deng H., Liu Y.H. (2014): Postantibiotic effect and postantibiotic sub-minimum inhibitory concentration effect of valnemulin against Staphylococcus aureus isolates from swine and chickens. Letters in Applied Microbiology, 58, 150-155 https://doi.org/10.1111/lam.12168
 
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