Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca)

https://doi.org/10.17221/125/2016-VETMEDCitation:Stancova V., Plhalova L., Blahova J., Zivna D., Bartoskova M., Siroka Z., Marsalek P., Svobodova Z. (2017): Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca). Veterinarni Medicina, 62: 90-97.
download PDF
In the present study, the effects of sub-lethal sub-chronic doses of ibuprofen, diclofenac, and carbamazepine alone, and in combination (concentration range 0.02–60 µg/l), on the early life stages of tench (Tinca tinca) were investigated. The lower concentrations of pharmaceuticals tested (0.02, 0.2, 2 µg/l) represent the concentration values of these substances commonly present in surface waters or effluents from wastewater treatment plants. Multiple biomarkers of biotransformation, antioxidant defence systems, and lipid peroxidation were determined in fish after 35 days of exposure. The evaluated pharmaceuticals induced oxidative stress in fish both alone and in combination with each other. Generally, 60 µg/l of each single pharmaceutical influenced the activity of antioxidant enzymes significantly (P < 0.05), whereas the same concentration of these pharmaceuticals in combination (1 : 1 : 1) did not have any impact on the activity of these enzymes. However, changes in biotransformation and antioxidant enzymes were apparent if lower concentrations of these pharmaceuticals were administered in the mixture. Significant changes (P < 0.05) in the activities of glutathione reductase, glutathione peroxidase, and glutathione-S-transferase were observed even at environmental concentration ranges. A significant effect (P < 0.05) on lipid peroxidation levels was found only in the experimental group exposed to carbamazepine.
Aebi H (1984): Catalase in vitro. Methods in Enzymology 105, 121–126.
Almeida Ângela, Calisto Vânia, Esteves Valdemar I., Schneider Rudolf J., M</b> <i>Soares Amadeu M.V., Figueira Etelvina, Freitas Rosa (2014): Presence of the pharmaceutical drug carbamazepine in coastal systems: effects on bivalves. Aquatic Toxicology, , -  https://doi.org/10.1016/j.aquatox.2014.08.002
Bartoskova M, Dobsikova R, Stancova V, Zivna D, Blahova J, Marsalek P, Zelnickova L, Bartos M, di Tocco FC, Faggio C (2013): Evaluation of ibuprofen toxicity for zebrafish (Danio rerio) targeting on selected biomarkers of oxidative stress. Neuroendocrinology Letters 34, 102–108.
Besse JP, Garric J (2008): Human pharmaceuticals in surface waters. Implementation of a prioriization methodology and application to the French situation. Toxicology Letters 176, 104–123.
Bhandari K, Venables B (2011): Ibuprofen bioconcentration and prostaglandin E2 levels in the bluntnose minnow Pimephales notatus COX. Comparative Biochemistry and Physiology, Part C 153, 251–257.
Brandão F.P., Rodrigues S., Castro B.B., Gonçalves F., Antunes S.C., Nunes B. (2013): Short-term effects of neuroactive pharmaceutical drugs on a fish species: Biochemical and behavioural effects. Aquatic Toxicology, 144-145, 218-229  https://doi.org/10.1016/j.aquatox.2013.10.005
Brozinski Jenny-Maria, Lahti Marja, Meierjohann Axel, Oikari Aimo, Kronberg Leif (2013): The Anti-Inflammatory Drugs Diclofenac, Naproxen and Ibuprofen are found in the Bile of Wild Fish Caught Downstream of a Wastewater Treatment Plant. Environmental Science & Technology, 47, 342-348  https://doi.org/10.1021/es303013j
Carlberg I, Mannervik B (1975): Purification and characterization of the flavoenzyme glutathione reductase from rat liver. The Journal of Biological Chemistry 250, 5475–5480.
David Anuradha, Pancharatna Katti (2009): Developmental anomalies induced by a non-selective COX inhibitor (ibuprofen) in zebrafish (Danio rerio). Environmental Toxicology and Pharmacology, 27, 390-395  https://doi.org/10.1016/j.etap.2009.01.002
Deeti Sudhakar, O'Farrell Sean, Kennedy Breandán N. (2014): Early safety assessment of human oculotoxic drugs using the zebrafish visualmotor response. Journal of Pharmacological and Toxicological Methods, 69, 1-8  https://doi.org/10.1016/j.vascn.2013.09.002
Ercegovac Marko, Jović Nebojša, Simić Tatjana, Beslać-Bumbaširević Ljiljana, Sokić Dragoslav, Savić-Radojević Ana, Matić Marija, Jovanović Dejana, Ristić Aleksandar, Đukić Tatjana, Šuvakov Sonja, Ćorić Vesna, MimiĆ-Oka Jasmina, Plješa-Ercegovac Marija (2013): Antiepileptic Drugs Affect Protein, Lipid and DNA Oxidative Damage and Antioxidant Defense in Patients with Epilepsy. Journal of Medical Biochemistry, 32, -  https://doi.org/10.2478/jomb-2013-0009
Flohe L, Gunzler WA (1984): Assays of glutathione peroxidase. Methods in Enzymology 105, 114–121.
Gómez-Oliván Leobardo Manuel, Neri-Cruz Nadia, Galar-Martínez Marcela, Islas-Flores Hariz, García-Medina Sandra (): Binary mixtures of diclofenac with paracetamol, ibuprofen, naproxen, and acetylsalicylic acid and these pharmaceuticals in isolated form induce oxidative stress on Hyalella azteca. Environmental Monitoring and Assessment, , -  https://doi.org/10.1007/s10661-014-3925-0
Habig WH, Pabst MJ, Jacoby WB (1974): Glutathione S-transferases. First enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry 249, 7130–7139.
Hong Han Na, Kim Han Na, Park Kyeong Seo, Lee Sung-Kyu, Gu Man Bock (2007): Analysis of the effects diclofenac has on Japanese medaka (Oryzias latipes) using real-time PCR. Chemosphere, 67, 2115-2121  https://doi.org/10.1016/j.chemosphere.2006.12.090
Khan Safdar A., McLean Mary Kay (2012): Toxicology of Frequently Encountered Nonsteroidal Anti-Inflammatory Drugs in Dogs and Cats. Veterinary Clinics of North America: Small Animal Practice, 42, 289-306  https://doi.org/10.1016/j.cvsm.2012.01.003
Láng Júlia, Kőhidai László (2012): Effects of the aquatic contaminant human pharmaceuticals and their mixtures on the proliferation and migratory responses of the bioindicator freshwater ciliate Tetrahymena. Chemosphere, 89, 592-601  https://doi.org/10.1016/j.chemosphere.2012.05.058
Lascelles B Duncan X, Court Michael H, Hardie Elizabeth M, Robertson Sheilah A (2007): Nonsteroidal anti-inflammatory drugs in cats: a review. Veterinary Anaesthesia and Analgesia, 34, 228-250  https://doi.org/10.1111/j.1467-2995.2006.00322.x
Li Zhi-Hua, Zlabek Vladimir, Velisek Josef, Grabic Roman, Machova Jana, Kolarova Jitka, Li Ping, Randak Tomas (2011): Acute toxicity of carbamazepine to juvenile rainbow trout (Oncorhynchus mykiss): Effects on antioxidant responses, hematological parameters and hepatic EROD. Ecotoxicology and Environmental Safety, 74, 319-327  https://doi.org/10.1016/j.ecoenv.2010.09.008
Li Zhihua, Lu Guanghua, Yang Xiaofan, Wang Chao (2012): Single and combined effects of selected pharmaceuticals at sublethal concentrations on multiple biomarkers in Carassius auratus. Ecotoxicology, 21, 353-361  https://doi.org/10.1007/s10646-011-0796-9
Lushchak Volodymyr I., Bagnyukova Tetyana V., Lushchak Oleh V., Storey Janet M., Storey Kenneth B. (2005): Hypoxia and recovery perturb free radical processes and antioxidant potential in common carp (Cyprinus carpio) tissues. The International Journal of Biochemistry & Cell Biology, 37, 1319-1330  https://doi.org/10.1016/j.biocel.2005.01.006
Nava-Álvarez R., Razo-Estrada A. C., García-Medina S., Gómez-Olivan L. M., Galar-Martínez M. (2014): Oxidative Stress Induced by Mixture of Diclofenac and Acetaminophen on Common Carp (Cyprinus carpio). Water, Air, & Soil Pollution, 225, -  https://doi.org/10.1007/s11270-014-1873-5
OECD – Organization for Economic Cooperation and Development (1992): Test No. 210: Fish, early-life stage toxicity test. OECD Guidelines for the testing of chemicals, Section 2. OECD Publishing, Paris.
Pomati F., Orlandi C., Clerici M., Luciani F., Zuccato E. (2007): Effects and Interactions in an Environmentally Relevant Mixture of Pharmaceuticals. Toxicological Sciences, 102, 129-137  https://doi.org/10.1093/toxsci/kfm291
Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. (1985): Measurement of protein using bicinchoninic acid. Analytical Biochemistry, 150, 76-85  https://doi.org/10.1016/0003-2697(85)90442-7
Stancova Vlasta, Plhalova Lucie, Bartoskova Marta, Zivna Dana, Prokes Miroslav, Marsalek Petr, Blahova Jana, Skoric Misa, Svobodova Zdenka (2014): Effects of Mixture of Pharmaceuticals on Early Life Stages of Tench (Tinca tinca). BioMed Research International, 2014, 1-10  https://doi.org/10.1155/2014/253468
Tran Ngoc Han, Urase Taro, Ta Thi Thao (2014): A Preliminary Study on the Occurrence of Pharmaceutically Active Compounds in Hospital Wastewater and Surface Water in Hanoi, Vietnam. CLEAN - Soil, Air, Water, 42, 267-275  https://doi.org/10.1002/clen.201300021
Tsiaka Pinelopi, Tsarpali Vasiliki, Ntaikou Ioanna, Kostopoulou Maria N., Lyberatos Gerasimos, Dailianis Stefanos (2013): Carbamazepine-mediated pro-oxidant effects on the unicellular marine algal species Dunaliella tertiolecta and the hemocytes of mussel Mytilus galloprovincialis. Ecotoxicology, 22, 1208-1220  https://doi.org/10.1007/s10646-013-1108-3
Zivna D, Plhalova L, Praskova E, Stepanova S, Siroka Z, Sevcikova M, Blahova J, Bartoskova M, Marsalek P, Skoric M, Svobodova Z (2013): Oxidative stress parameters in fish after subchronic exposure to acetylsalicylic acid. Neuroendocrinology Letters 34, 116–122.
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti